A6UEF5 (AROE_SINMW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Shikimate dehydrogenase
EC=1.1.1.25

Gene names

Name:	aroE
Ordered Locus Names:	Smed_3211

Organism

Sinorhizobium medicae (strain WSM419) (Ensifer medicae) [Complete proteome] [HAMAP]

Taxonomic identifier

366394 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Shikimate + NADP+ = 3-dehydroshikimate + NADPH. HAMAP MF_00222
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7. HAMAP MF_00222
Sequence similarities	Belongs to the shikimate dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro shikimate 3-dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 286	286	Shikimate dehydrogenase HAMAP MF_00222		PRO_1000100142
Regions
	Nucleotide binding	136 – 140	5	NADP By similarity
Sites
	Active site	75	1	Proton acceptor Potential

Sequences

	Sequence		Length	Mass (Da)
	A6UEF5 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: E03B599013814523	FASTA	286	31,179
	10 20 30 40 50 60 MRDSRETFVN HAFVAGYPVK HSRSPLIHGY WLKRFGIQGS YRAHEVTSDA FPDFMRQIKE 70 80 90 100 110 120 GTAGFCGGNV TIPHKEAAFR LSDRPDELSA ELGAANTLWL ENGTIRATNT DGRGFVANLD 130 140 150 160 170 180 ERAKGWDRIS AAVILGAGGA SRAVIQAIRD RGVKAIHVVN RTPERARELA DRFGSAVHAH 190 200 210 220 230 240 SMAALPEVLS GAGLFVNTTS LGMDGEPAPA IDFSKLARDA VVTDIVYVPL KTPLLRQAEE 250 260 270 280 QGFRVVDGLG MLLHQAAPGF EKWFGLRPVV DEALREIIIS DMDKHA

References

[1]

"Complete sequence of Sinorhizobium medicae WSM419 chromosome." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Reeve W.G., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: WSM419.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000738 Genomic DNA. Translation: ABR62035.1.
RefSeq	YP_001328870.1. NC_009636.1.
3D structure databases
ProteinModelPortal	A6UEF5.
ModBase	Search...
Protein-protein interaction databases
STRING	A6UEF5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5324090.
GenomeReviews	Gene locus Smed_3211 in contig CP000738_GR.
KEGG	smd:Smed_3211.
PATRIC	23625663. VBISinMed134228_6449.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0169.
HOGENOM	HBG553408.
OMA	FERWFGI.
ProtClustDB	PRK00258.
Enzyme and pathway databases
BioCyc	SMED366394:SMED_3211-MONOMER.
Family and domain databases
HAMAP	MF_00222. Shikimate_DH_AroE. [Tree]
InterPro	IPR016040. NAD(P)-bd_dom. IPR011342. Shikimate_DH. IPR013708. Shikimate_DH-bd_N. IPR022893. Shikimate_quinate_DH. IPR006151. Shikm_DH/Glu-tRNA_Rdtase. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00014.
Pfam	PF01488. Shikimate_DH. 1 hit. PF08501. Shikimate_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00507. AroE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROE_SINMW

Accession

Primary (citable) accession number: A6UEF5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	August 21, 2007
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program