A6UDW8 (DNAE2_SINMW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Error-prone DNA polymerase
EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	Smed_3021

Organism

Sinorhizobium medicae (strain WSM419) (Ensifer medicae) [Complete proteome] [HAMAP]

Taxonomic identifier

366394 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	1116 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity. HAMAP MF_01902
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm By similarity HAMAP MF_01902.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1116	1116	Error-prone DNA polymerase HAMAP MF_01902		PRO_1000070598

Sequences

	Sequence		Length	Mass (Da)
	A6UDW8 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: EFEAF91A4053FDD2	FASTA	1,116	124,942
	10 20 30 40 50 60 MSGDTAFYEL GACTNFSFLE GAAPAEEMVV FAKKARLAGL GIADRNSVAG VVRAHAKAKM 70 80 90 100 110 120 ENYPFQPGAR LVFADGTPDV LAYPRNRRGW GHLCRLLSAG NLRSKKGDCT LHLADLLEWQ 130 140 150 160 170 180 EELLLIVMPD RVRPEPESLK PLLGKLQEHA GNRLYLGLAP RYDGFDRHDF AVLATVARKA 190 200 210 220 230 240 GIGLLATNDA LYHDPDYRPL ADVVTAIREH VPVAGAGFLL QKNAERHLKS PREMARLFSD 250 260 270 280 290 300 YPEAIANTQK FFRHLAFSLD ELRHQYPDEN AGGETPAESL RRLVSEGAAE RYPEGVPEKV 310 320 330 340 350 360 QRQIEYELEL INDKKYEPYF LTVHKLVKFA RSEKILCQGR GSAANSSVCF CLGITDVDPQ 370 380 390 400 410 420 KFTLLFDRFL SKDRDEPPDI DVDFEHERRE EVIQYIYRTY GKEHAGLAAA VISYRSRSAG 430 440 450 460 470 480 REVAKAFGFS EDVQSALVSS IWGWGNSPFT EEQARGAGLD AADPSTRRVL AYASLLMNYP 490 500 510 520 530 540 RHLSQHVGGF VITRDRLDEV VPIMNTAMPD RYMIEWDKDD LDELKILKVD VLALGMLTCL 550 560 570 580 590 600 AKGFKLLEAH YGEPITLAEI YQDHQPAVYD MICRADTVGV FQIESRAQMS MLPRLQPREM 610 620 630 640 650 660 YDLVIEVAIV RPGPIQGNMV HPYLKRREGQ RKGEKVKYPS PELKAVLERT LGVPLFQEQA 670 680 690 700 710 720 MQIAITAAGF SPSEADRLRR AMATFKRTGT IHTFERKMVE GMVANGYERE FAERCFNQIK 730 740 750 760 770 780 GFGEYGFPES HAASFASLVY ASAWLKTYYP DIFCAALLNA QPMGFYAPAQ LVRDAREHGV 790 800 810 820 830 840 KVLPVDINHS DWDALLEGEG QFRKESVDPR HADMREVIKT RKAVRLGFRL VKGLKQADMG 850 860 870 880 890 900 ALVACRGEGY RSVHDLWFRS GLSRSVLERL ADADAFRSLG LDRRAALWAV KALDEQSAVE 910 920 930 940 950 960 RLPLFEGAGS LDLRAEPKVA LPEMPAGEQV IHDYRTLTLS LKAHPVSFMR EDFSRTGILR 970 980 990 1000 1010 1020 SRDLAATATG KWVTVAGLVL VRQRPGSANG VIFMTIEDET GIANIIVWEK TFRKYRPQVM 1030 1040 1050 1060 1070 1080 GSRLVKIRGR LQNQSGVIHV VADHLEDITP MLGLLRREAR RFGANDRADG ALRPSGDARE 1090 1100 1110 KRKLRQLRLG LPGGAEPEGE AAAQVAEAMP KGRNFH

References

[1]

"Complete sequence of Sinorhizobium medicae WSM419 chromosome." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Reeve W.G., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: WSM419.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000738 Genomic DNA. Translation: ABR61848.1.
RefSeq	YP_001328683.1. NC_009636.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6UDW8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5323899.
GenomeReviews	Gene locus Smed_3021 in contig CP000738_GR.
KEGG	smd:Smed_3021.
PATRIC	23625254. VBISinMed134228_6246.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0587.
HOGENOM	HBG734490.
OMA	LATNRNG.
ProtClustDB	CLSK784100.
Enzyme and pathway databases
BioCyc	SMED366394:SMED_3021-MONOMER.
Family and domain databases
HAMAP	MF_01902. DNApol_error_prone. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR023073. DNA_pol_error_prone. IPR004365. NA-bd_OB_tRNA-helicase. IPR004805. PolC_alpha. [Graphical view]
KO	K14162.
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. Polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_SINMW

Accession

Primary (citable) accession number: A6UDW8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	August 21, 2007
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program