Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Sinorhizobium medicae (strain WSM419) (Ensifer medicae)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei256ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:Smed_2799
OrganismiSinorhizobium medicae (strain WSM419) (Ensifer medicae)
Taxonomic identifieri366394 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
Proteomesi
  • UP000001108 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003309981 – 485Glutamate--tRNA ligaseAdd BLAST485

Proteomic databases

PRIDEiA6UD99

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi366394.Smed_2799

Structurei

3D structure databases

ProteinModelPortaliA6UD99
SMRiA6UD99
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi12 – 22"HIGH" regionUniRule annotationAdd BLAST11
Motifi253 – 257"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252720
KOiK01885
OMAiAYYAFDT
OrthoDBiPOG091H021W

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A6UD99-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADSAVRVRI APSPTGEPHV GTAYIALFNY LFAKKHGGEF ILRIEDTDAT
60 70 80 90 100
RSTPEFEKKV LDALKWCGLE WSEGPDIGGP CGPYRQSDRK DIYKPYVEQI
110 120 130 140 150
VANGHGFRCF CTPERLEQMR EAQRAAGKPP KYDGLCLSLS AEEVTSRVAA
160 170 180 190 200
GEPHVVRMKI PTEGSCKFRD GVYGDVEIPW EAVDMQVLLK ADGMPTYHMA
210 220 230 240 250
NVVDDHLMKI THVARGEEWL ASVPKHILIY QYLGLEPPVF MHLSLMRNAD
260 270 280 290 300
KSKLSKRKNP TSISYYTALG YLPEALMNFL GLFFIQIAEG EELLTMAELA
310 320 330 340 350
EKFDPENLSK AGAIFDIQKL DWLNARWIRE KLSEEQFAAK VLSWAMDNDR
360 370 380 390 400
LREGLKLSQT RISKLGELPD LAAFLFKSDL GLQPAAFAGV KASPEEMLEI
410 420 430 440 450
LNTVQPDLEK ILEWNKESIE TELRACAERM GKKLKAVVAP LFVAVSGSQR
460 470 480
SLPLFDSMEL LGRSVVRQRL KVAAQVVASM AGSGK
Length:485
Mass (Da):54,167
Last modified:August 21, 2007 - v1
Checksum:i1900F4586891D4E5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000738 Genomic DNA Translation: ABR61629.1
RefSeqiWP_012067014.1, NC_009636.1
YP_001328464.1, NC_009636.1

Genome annotation databases

EnsemblBacteriaiABR61629; ABR61629; Smed_2799
GeneIDi5323669
KEGGismd:Smed_2799
PATRICifig|366394.8.peg.6006

Similar proteinsi

Entry informationi

Entry nameiSYE_SINMW
AccessioniPrimary (citable) accession number: A6UD99
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: August 21, 2007
Last modified: May 23, 2018
This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health