A6UCK2 (A6UCK2_SINMW) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Hydroxyacylglutathione hydrolase HAMAP MF_01374
EC=3.1.2.6 HAMAP MF_01374

Alternative name(s):
Glyoxalase II HAMAP MF_01374

Gene names

Name:	gloB HAMAP MF_01374
Ordered Locus Names:	Smed_2552

Organism

Sinorhizobium medicae (strain WSM419) (Ensifer medicae) [Complete proteome] [HAMAP]

Taxonomic identifier

366394 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374 SAAS SAAS017782
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374 SAAS SAAS017782
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374 SAAS SAAS017782
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374
Subunit structure	Monomer By similarity. HAMAP MF_01374 SAAS SAAS017782
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family. HAMAP MF_01374

Ontologies

Keywords
Ligand	Metal-binding HAMAP MF_01374 SAAS SAAS017782 Zinc HAMAP MF_01374 SAAS SAAS017782
Molecular function	Hydrolase HAMAP MF_01374 SAAS SAAS017782 EMBL ABR61382.1
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	57	1	Zinc 1 By similarity HAMAP MF_01374
	Metal binding	59	1	Zinc 1 By similarity HAMAP MF_01374
	Metal binding	61	1	Zinc 2 By similarity HAMAP MF_01374
	Metal binding	62	1	Zinc 2 By similarity HAMAP MF_01374
	Metal binding	115	1	Zinc 1 By similarity HAMAP MF_01374
	Metal binding	134	1	Zinc 1 By similarity HAMAP MF_01374
	Metal binding	134	1	Zinc 2 By similarity HAMAP MF_01374
	Metal binding	172	1	Zinc 2 By similarity HAMAP MF_01374

Sequences

	Sequence		Length	Mass (Da)
	A6UCK2 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: C3E2A72E0FFAD148	FASTA	256	28,312
	10 20 30 40 50 60 MAALELDLFL CRTDNYGVLL HDRLSGATAS IDAPEERPIL DALERRSWQL THILTTHHHG 70 80 90 100 110 120 DHVAGNIGLK ERFGVTIIGP KNESPKIPGI DRSVGHGDRF DFAGHPVEVI ETPGHTAGHV 130 140 150 160 170 180 CYHFPEDKLL FAADTLFALG CGRLFEGTPE TMWQSLSRLM ALPDDTAIYF GHEYTLANAR 190 200 210 220 230 240 FAVTVDPENT ALKERAAEIE ETRSDGGFTA PTTMGLEKRT NPFLRADDPK IRAFLGMDKA 250 SDAAVFAEIR KRKDSF

References

[1]

"Complete sequence of Sinorhizobium medicae WSM419 chromosome." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Reeve W.G., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: WSM419.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000738 Genomic DNA. Translation: ABR61382.1.
RefSeq	YP_001328217.1. NC_009636.1.
3D structure databases
ProteinModelPortal	A6UCK2.
ModBase	Search...
Protein-protein interaction databases
STRING	A6UCK2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5323420.
GenomeReviews	Gene locus Smed_2552 in contig CP000738_GR.
KEGG	smd:Smed_2552.
PATRIC	23624229. VBISinMed134228_5744.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0491.
HOGENOM	HBG753931.
OMA	FLCRTDN.
ProtClustDB	CLSK800959.
Enzyme and pathway databases
BioCyc	SMED366394:SMED_2552-MONOMER.
Family and domain databases
HAMAP	MF_01374. Glyoxalase_2. [Tree]
InterPro	IPR001279. Beta-lactamas-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
KO	K01069.
PANTHER	PTHR11935:SF7. PTHR11935:SF7. 1 hit.
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03413. GSH_gloB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A6UCK2_SINMW

Accession

Primary (citable) accession number: A6UCK2

Entry history

Integrated into UniProtKB/TrEMBL:	August 21, 2007
Last sequence update:	August 21, 2007
Last modified:	December 14, 2011
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)