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Protein

Formate--tetrahydrofolate ligase

Gene

fhs

Organism
Sinorhizobium medicae (strain WSM419) (Ensifer medicae)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.UniRule annotation

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi76 – 838ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. formate-tetrahydrofolate ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. folic acid-containing compound biosynthetic process Source: InterPro
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate--tetrahydrofolate ligaseUniRule annotation (EC:6.3.4.3UniRule annotation)
Alternative name(s):
Formyltetrahydrofolate synthetaseUniRule annotation
Short name:
FHSUniRule annotation
Short name:
FTHFSUniRule annotation
Gene namesi
Name:fhsUniRule annotation
Ordered Locus Names:Smed_2298
OrganismiSinorhizobium medicae (strain WSM419) (Ensifer medicae)
Taxonomic identifieri366394 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
ProteomesiUP000001108 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 567567Formate--tetrahydrofolate ligasePRO_0000318569Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi366394.Smed_2298.

Structurei

3D structure databases

ProteinModelPortaliA6UBV0.
SMRiA6UBV0. Positions 15-565.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the formate--tetrahydrofolate ligase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
OMAiCMVKTHL.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6UBV0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKQLGEAVG EVKTDIEIAR AARKQPIMEV GARLGIPPEH LLPYGHDKAK
60 70 80 90 100
VSAEFIAAQR EKKNGRLILV TAINPTPAGE GKTTTTVGLV DGLNRIGKKA
110 120 130 140 150
IVCIREASLG PCFGIKGGAA GGGYAQVVPM EDINLHFTGD FHAITSAHNL
160 170 180 190 200
LSALIDNHIY WGNEQAIDIR RIAWRRVMDM NDRALRHIVG SLGGVANGYP
210 220 230 240 250
RETGFDITVA SEVMAILCLA MDIRDLERRL GNIIIGYRRD KSPVYARDIK
260 270 280 290 300
ADGAMAVLLK DAMQPNLVQT LENNPAFVHG GPFANIAHGC NSVVATTTAL
310 320 330 340 350
KLADYVVTEA GFGADLGAEK FFDIKCRKAG LMPDAAVIVA TVRAIKMNGG
360 370 380 390 400
VKKEDLAKEN VEALRKGCPN LGRHIQNVKK FGVPVLVAIN HFTSDTEAEI
410 420 430 440 450
QAIKDYVRTL GSEAVLCKHW AEGSAGIEEL ADKVADLADA GHSQFSPLYP
460 470 480 490 500
DEMPLFQKIE AIAKDIYHAS EVIADKLVRD QLRIWEDQGY GHLPICMAKT
510 520 530 540 550
QYSFSTDPNL RGAPTGHTVP IREVRLAAGA GFIVVITGEI MTMPGLPKVP
560
SSERIRLDEE GYIEGLF
Length:567
Mass (Da):61,461
Last modified:August 21, 2007 - v1
Checksum:i112175D55AA5B11A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000738 Genomic DNA. Translation: ABR61130.1.

Genome annotation databases

EnsemblBacteriaiABR61130; ABR61130; Smed_2298.
PATRICi23623677. VBISinMed134228_5475.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000738 Genomic DNA. Translation: ABR61130.1.

3D structure databases

ProteinModelPortaliA6UBV0.
SMRiA6UBV0. Positions 15-565.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi366394.Smed_2298.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABR61130; ABR61130; Smed_2298.
PATRICi23623677. VBISinMed134228_5475.

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
OMAiCMVKTHL.

Enzyme and pathway databases

UniPathwayiUPA00193.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of Sinorhizobium medicae WSM419 chromosome."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Reeve W.G., Richardson P.
    Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: WSM419.

Entry informationi

Entry nameiFTHS_SINMW
AccessioniPrimary (citable) accession number: A6UBV0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: August 21, 2007
Last modified: January 7, 2015
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.