A6UBV0 (FTHS_SINMW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate--tetrahydrofolate ligase
EC=6.3.4.3

Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	Smed_2298

Organism

Sinorhizobium medicae (strain WSM419) (Ensifer medicae) [Complete proteome] [HAMAP]

Taxonomic identifier

366394 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	567 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 567	567	Formate--tetrahydrofolate ligase HAMAP MF_01543		PRO_0000318569
Regions
	Nucleotide binding	76 – 83	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6UBV0 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 112175D55AA5B11A	FASTA	567	61,461
	10 20 30 40 50 60 MRKQLGEAVG EVKTDIEIAR AARKQPIMEV GARLGIPPEH LLPYGHDKAK VSAEFIAAQR 70 80 90 100 110 120 EKKNGRLILV TAINPTPAGE GKTTTTVGLV DGLNRIGKKA IVCIREASLG PCFGIKGGAA 130 140 150 160 170 180 GGGYAQVVPM EDINLHFTGD FHAITSAHNL LSALIDNHIY WGNEQAIDIR RIAWRRVMDM 190 200 210 220 230 240 NDRALRHIVG SLGGVANGYP RETGFDITVA SEVMAILCLA MDIRDLERRL GNIIIGYRRD 250 260 270 280 290 300 KSPVYARDIK ADGAMAVLLK DAMQPNLVQT LENNPAFVHG GPFANIAHGC NSVVATTTAL 310 320 330 340 350 360 KLADYVVTEA GFGADLGAEK FFDIKCRKAG LMPDAAVIVA TVRAIKMNGG VKKEDLAKEN 370 380 390 400 410 420 VEALRKGCPN LGRHIQNVKK FGVPVLVAIN HFTSDTEAEI QAIKDYVRTL GSEAVLCKHW 430 440 450 460 470 480 AEGSAGIEEL ADKVADLADA GHSQFSPLYP DEMPLFQKIE AIAKDIYHAS EVIADKLVRD 490 500 510 520 530 540 QLRIWEDQGY GHLPICMAKT QYSFSTDPNL RGAPTGHTVP IREVRLAAGA GFIVVITGEI 550 560 MTMPGLPKVP SSERIRLDEE GYIEGLF

References

[1]

"Complete sequence of Sinorhizobium medicae WSM419 chromosome." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Reeve W.G., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: WSM419.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000738 Genomic DNA. Translation: ABR61130.1.
RefSeq	YP_001327965.2. NC_009636.1.
3D structure databases
ProteinModelPortal	A6UBV0.
SMR	A6UBV0. Positions 15-565.
ModBase	Search...
Protein-protein interaction databases
STRING	A6UBV0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5323159.
GenomeReviews	Gene locus Smed_2298 in contig CP000738_GR.
KEGG	smd:Smed_2298.
PATRIC	23623677. VBISinMed134228_5475.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2759.
HOGENOM	HBG677721.
OMA	TDFTVTI.
ProtClustDB	PRK13505.
Enzyme and pathway databases
BioCyc	SMED366394:SMED_2298-MONOMER.
Family and domain databases
HAMAP	MF_01543. FTHFS. [Tree]
InterPro	IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. [Graphical view]
KO	K01938.
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_SINMW

Accession

Primary (citable) accession number: A6UBV0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	August 21, 2007
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program