A6UBM6 (TDH_SINMW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L-threonine 3-dehydrogenase
EC=1.1.1.103

Gene names

Name:	tdh
Ordered Locus Names:	Smed_2224

Organism

Sinorhizobium medicae (strain WSM419) (Ensifer medicae) [Complete proteome] [HAMAP]

Taxonomic identifier

366394 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-threonine + NAD+ = L-2-amino-3-oxobutanoate + NADH. HAMAP MF_00627
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_00627
Pathway	Amino-acid degradation; L-threonine degradation via oxydo-reductase pathway; glycine from L-threonine: step 1/2. HAMAP MF_00627
Subunit structure	Homotetramer By similarity. HAMAP MF_00627
Subcellular location	Cytoplasm By similarity HAMAP MF_00627.
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonine catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-threonine 3-dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	L-threonine 3-dehydrogenase HAMAP MF_00627		PRO_1000051665
Sites
	Metal binding	42	1	Zinc 1; catalytic By similarity
	Metal binding	67	1	Zinc 1; catalytic By similarity
	Metal binding	97	1	Zinc 2 By similarity
	Metal binding	100	1	Zinc 2 By similarity
	Metal binding	103	1	Zinc 2 By similarity
	Metal binding	111	1	Zinc 2 By similarity
	Metal binding	152	1	Zinc 1; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6UBM6 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 06F161F75CE1608C	FASTA	344	37,564
	10 20 30 40 50 60 MTNMMRALVK TRPEVGLWME RVPVPEVGPN DVLIRVRKSA ICGTDVHIWN WDQWAQKTIP 70 80 90 100 110 120 VPMVVGHEFM GEIVEVGPAV TKHHVGERVS GEGHIVCGKC RNCRAGRGHL CRNTLGVGVN 130 140 150 160 170 180 RPGSFAEFVC LPEYNVVSIP DDVPDEIAAI FDPFGNAVHT ALSFDLVGED VLVTGAGPIG 190 200 210 220 230 240 IMGALVAKRC GARKVVITDI NPVRLDLARK VGIDYVVDAS KENLADVMRV IGMTEGFDVG 250 260 270 280 290 300 LEMSGAAPAF RDMIDKMNNG GKIAILGIAP AGFEIDWNKV IFKMLNLKGI YGREMFETWY 310 320 330 340 KMIAFVQGGL DLSQIITHRI GIDEFGDGFE AMRSGNSGKV VMDW

References

[1]

"Complete sequence of Sinorhizobium medicae WSM419 chromosome." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Reeve W.G., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: WSM419.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000738 Genomic DNA. Translation: ABR61056.1.
RefSeq	YP_001327891.1. NC_009636.1.
3D structure databases
ProteinModelPortal	A6UBM6.
ModBase	Search...
Protein-protein interaction databases
STRING	A6UBM6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5323085.
GenomeReviews	Gene locus Smed_2224 in contig CP000738_GR.
KEGG	smd:Smed_2224.
PATRIC	23623511. VBISinMed134228_5392.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1063.
HOGENOM	HBG753318.
OMA	MMRSGMS.
ProtClustDB	PRK05396.
Enzyme and pathway databases
BioCyc	SMED366394:SMED_2224-MONOMER.
Family and domain databases
HAMAP	MF_00627. Thr_dehydrog. [Tree]
InterPro	IPR013149. ADH_C. IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn-type. IPR002328. ADH_Zn_CS. IPR011032. GroES-like. IPR004627. L-Threonine_3-DHase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00060.
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
SUPFAM	SSF50129. GroES_like. 1 hit.
TIGRFAMs	TIGR00692. Tdh. 1 hit.
PROSITE	PS00059. ADH_ZINC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TDH_SINMW

Accession

Primary (citable) accession number: A6UBM6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 21, 2007
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program