A6UB89 (A6UB89_SINMW) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase RuleBase RU004136
EC=6.3.2.10 RuleBase RU004136

Gene names

Ordered Locus Names:

Smed_2086 EMBL ABR60919.1

Organism

Sinorhizobium medicae (strain WSM419) (Ensifer medicae) [Complete proteome] [HAMAP] EMBL ABR60919.1

Taxonomic identifier

366394 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium › Sinorhizobium medicae

Protein attributes

Sequence length	477 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the MurCDEF family. RuleBase RU003630

Ontologies

Keywords
Biological process	Cell cycle Cell division RuleBase RU004136 Cell shape Cell wall biogenesis/degradation RuleBase RU004136 Peptidoglycan synthesis RuleBase RU004136
Ligand	ATP-binding RuleBase RU003630 Nucleotide-binding
Molecular function	Ligase RuleBase RU003630 EMBL ABR60919.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity Inferred from electronic annotation. Source: EC UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	A6UB89 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 516C40CEA7B6A4BB	FASTA	477	49,808
	10 20 30 40 50 60 MNWLWTSSDL LGAMNGRPVG NLPEGITGIS IDSRTIGNGE AFFAIRGDRV DGHDYAGIAL 70 80 90 100 110 120 ANGAALLVVS EAKLPALGRL IAPMIVVDDV LEAMIRLGCA ARDRSAAKII AVTGSVGKTT 130 140 150 160 170 180 TKEMLRHMLS PLGRVHASVA SFNNHWGVPL TLARMPETTD YGVFEIGMNH AGEIRPLTRM 190 200 210 220 230 240 VRPHVAVVTS IAAAHLGNFA SLDDIAAAKA EIFEGVVNGG HAVINRDSAQ YELLEKAAAT 250 260 270 280 290 300 AGVSHVHFFG ANPKSEFRLA EFAGGAEGSV LWAGVGGRTL EIAIGAPGRH IAENALAAIA 310 320 330 340 350 360 AATLAGADTD RVAASLATMQ PEKGRGLRHR LRMGAGYMTL IDESYNANPA SMRAAITLLR 370 380 390 400 410 420 DAEPSPGGRR IAILGDMLEM GEHSAAVHAA LARPIVDAGI ADVWLTGTEM AHLRDALPSE 430 440 450 460 470 VSVVHRDLVS DLTAYALGAV AAGDVVMVKS SKSTGCAKIV QALLDAHPHD EAGKREA

References

[1]

"Complete sequence of Sinorhizobium medicae WSM419 chromosome." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Reeve W.G., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: WSM419.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000738 Genomic DNA. Translation: ABR60919.1.
RefSeq	YP_001327754.1. NC_009636.1.
3D structure databases
ProteinModelPortal	A6UB89.
ModBase	Search...
Protein-protein interaction databases
STRING	366394.Smed_2086.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABR60919; ABR60919; Smed_2086.
GeneID	5322945.
KEGG	smd:Smed_2086.
PATRIC	23623213. VBISinMed134228_5244.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0770.
HOGENOM	HOG000268120.
KO	K01929.
OMA	HCVGPRM.
ProtClustDB	CLSK863318.
Enzyme and pathway databases
BioCyc	SMED366394:GJAL-2118-MONOMER.
UniPathway	UPA00219.
Family and domain databases
Gene3D	3.40.1190.10. 1 hit. 3.90.190.20. 1 hit.
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR005863. UDP-N-AcMur-pentapeptide_synth. [Graphical view]
PANTHER	PTHR23135:SF3. PTHR23135:SF3. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01143. murF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A6UB89_SINMW

Accession

Primary (citable) accession number: A6UB89

Entry history

Integrated into UniProtKB/TrEMBL:	August 21, 2007
Last sequence update:	August 21, 2007
Last modified:	May 1, 2013
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)