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Protein

Fumarate hydratase class II

Gene

fumC

Organism
Sinorhizobium medicae (strain WSM419) (Ensifer medicae)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.UniRule annotation

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.UniRule annotation

Catalytic activityi

(S)-malate = fumarate + H2O.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Fumarate hydratase class II (fumC)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei206SubstrateUniRule annotation1
Active sitei207Proton donor/acceptorUniRule annotation1
Active sitei337UniRule annotation1
Binding sitei338SubstrateUniRule annotation1
Sitei350Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotationSAAS annotation
Biological processTricarboxylic acid cycleUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00223; UER01007.

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class IIUniRule annotation (EC:4.2.1.2UniRule annotation)
Short name:
Fumarase CUniRule annotation
Alternative name(s):
Aerobic fumaraseUniRule annotation
Iron-independent fumaraseUniRule annotation
Gene namesi
Name:fumCUniRule annotation
Ordered Locus Names:Smed_1576Imported
OrganismiSinorhizobium medicae (strain WSM419) (Ensifer medicae)Imported
Taxonomic identifieri366394 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
Proteomesi
  • UP000001108 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi366394.Smed_1576.

Structurei

3D structure databases

ProteinModelPortaliA6U9T7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 361Lyase_1InterPro annotationAdd BLAST331
Domaini427 – 480FumaraseC_CInterPro annotationAdd BLAST54

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni117 – 119Substrate bindingUniRule annotation3
Regioni148 – 151Substrate binding (B site)UniRule annotation4
Regioni158 – 160Substrate bindingUniRule annotation3
Regioni343 – 345Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9Q. Bacteria.
COG0114. LUCA.
HOGENOMiHOG000061736.
KOiK01679.
OMAiVSFTDNC.
OrthoDBiPOG091H01XG.

Family and domain databases

CDDicd01362. Fumarase_classII. 1 hit.
Gene3Di1.10.275.10. 1 hit.
HAMAPiMF_00743. FumaraseC. 1 hit.
InterProiView protein in InterPro
IPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiView protein in Pfam
PF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
PRINTSiPR00149. FUMRATELYASE.
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR00979. fumC_II. 1 hit.
PROSITEiView protein in PROSITE
PS00163. FUMARATE_LYASES. 1 hit.

Sequencei

Sequence statusi: Complete.

A6U9T7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPAPRLGFM NRMMKAGITM TSTRTETDTF GPIEVASDRY WGAQAQRSLG
60 70 80 90 100
NFKIGWEKQP LAVVRALGIV KQAAARANMA LGRLDPTIGD AIVKAAQEVI
110 120 130 140 150
DGKLDDHFPL VVWQTGSGTQ SNMNANEVIS NRAIELLGGV MGSKKPVHPN
160 170 180 190 200
DHVNMSQSSN DTYPTAMHIA CAERVIHDLL PALKHLHKAL EEKVKAFQSI
210 220 230 240 250
IKIGRTHTQD ATPLTLGQEF SGYAAQVASS IKRIEMTLPG LCELAQGGTA
260 270 280 290 300
VGTGLNAPVG FAEKVAEQIA SITGIGFTSA PNKFEALAAH DSMVFSHGAI
310 320 330 340 350
NATAAALFKI ANDIRFLGSG PRSGLGELSL PENEPGSSIM PGKVNPTQCE
360 370 380 390 400
ALTQVCVQVF GNHASLTFAG SQGHFELNVY NPLMAYNYLQ SVQLLADAAV
410 420 430 440 450
SFTDNCVVGI EAREDNIKAA LDRSLMLVTA LAPKIGYDNA AKIAKTAHKN
460 470 480
GTTLRQEAVG GGYVTDEEFD AVVRPETMIG PA
Length:482
Mass (Da):51,277
Last modified:August 21, 2007 - v1
Checksum:i068F0496CCE62D1C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000738 Genomic DNA. Translation: ABR60417.1.
RefSeqiWP_011975725.1. NC_009636.1.
YP_001327252.1. NC_009636.1.

Genome annotation databases

EnsemblBacteriaiABR60417; ABR60417; Smed_1576.
GeneIDi5322434.
KEGGismd:Smed_1576.
PATRICifig|366394.8.peg.4714.

Similar proteinsi

Entry informationi

Entry nameiA6U9T7_SINMW
AccessioniPrimary (citable) accession number: A6U9T7
Entry historyiIntegrated into UniProtKB/TrEMBL: August 21, 2007
Last sequence update: August 21, 2007
Last modified: September 27, 2017
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported