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A6U8P9

- TRMFO_SINMW

UniProt

A6U8P9 - TRMFO_SINMW

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Protein
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO
Gene
trmFO, gid, Smed_1178
Organism
Sinorhizobium medicae (strain WSM419) (Ensifer medicae)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs By similarity.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotation

Cofactori

FAD By similarity.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi15 – 206FAD By similarity

GO - Molecular functioni

  1. 5,10-methylenetetrahydrofolate-dependent tRNA (m5U54) methyltransferase activity Source: UniProtKB-HAMAP
  2. flavin adenine dinucleotide binding Source: UniProtKB-HAMAP
  3. methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    BioCyciSMED366394:GJAL-1198-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO (EC:2.1.1.74)
    Alternative name(s):
    Folate-dependent tRNA (uracil-5-)-methyltransferase
    Folate-dependent tRNA(M-5-U54)-methyltransferase
    Gene namesi
    Name:trmFO
    Synonyms:gid
    Ordered Locus Names:Smed_1178
    OrganismiSinorhizobium medicae (strain WSM419) (Ensifer medicae)
    Taxonomic identifieri366394 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
    ProteomesiUP000001108: Chromosome

    Subcellular locationi

    Cytoplasm By similarity UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 480480Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotation
    PRO_1000063928Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi366394.Smed_1178.

    Structurei

    3D structure databases

    ProteinModelPortaliA6U8P9.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the MnmG family. TrmFO subfamily.

    Phylogenomic databases

    eggNOGiCOG1206.
    HOGENOMiHOG000252054.
    KOiK04094.
    OMAiWEMRAAG.
    OrthoDBiEOG6J74VT.

    Family and domain databases

    HAMAPiMF_01037. TrmFO.
    InterProiIPR004417. Folate-dep_Ribothymidyl_synth.
    IPR002218. GIDA-rel.
    IPR020595. GIDA-rel_CS.
    [Graphical view]
    PfamiPF01134. GIDA. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
    PROSITEiPS01281. GIDA_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A6U8P9-1 [UniParc]FASTAAdd to Basket

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    MNKLTSSYSP VHVIGGGLAG SEAAWQIAEA GVPVILHEMR GVRGTDAHKT    50
    DSLAELVCSN SFRSDDATSN AVGVLHAEMR LAGSIIMRCA DLNQVPAGGA 100
    LAVDREGFAE AVSAAIAAHP LITVLREEIR GLPPKEWDLA IIATGPLTAP 150
    DLADAIRAET GADALAFFDA IAPIVHADTI DMDICWHQSR YDKVGPGGTG 200
    KDYINCPLTQ EEYNAFIDAL IAGDKTGFKE WEGTPYFDGC LPIEVMAERG 250
    RETLRHGPMK PMGLTNAHNP SVKPYAVVQL RQDNALGTLY NMVGFQTKLK 300
    YGAQGEVFRM IPGLEKAEFA RLGGLHRNTY INSPTLLDQS LTLKSRAGLR 350
    FAGQITGCEG YVESASIGLL AGRFAAAERK GASPAIPPVT TAFGALLNHI 400
    TGGHIVSDDE PGKRSFQPMN VNFGLFPPLD PGALTRPEGA KRFRGKEKAS 450
    AKKQAMASRA LSDCATWLDK NLLIGGAATR 480
    Length:480
    Mass (Da):51,129
    Last modified:August 21, 2007 - v1
    Checksum:iAA6A7587022C1B28
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000738 Genomic DNA. Translation: ABR60029.1.
    RefSeqiYP_001326864.1. NC_009636.1.

    Genome annotation databases

    EnsemblBacteriaiABR60029; ABR60029; Smed_1178.
    GeneIDi5322024.
    KEGGismd:Smed_1178.
    PATRICi23621306. VBISinMed134228_4305.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000738 Genomic DNA. Translation: ABR60029.1 .
    RefSeqi YP_001326864.1. NC_009636.1.

    3D structure databases

    ProteinModelPortali A6U8P9.
    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 366394.Smed_1178.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABR60029 ; ABR60029 ; Smed_1178 .
    GeneIDi 5322024.
    KEGGi smd:Smed_1178.
    PATRICi 23621306. VBISinMed134228_4305.

    Phylogenomic databases

    eggNOGi COG1206.
    HOGENOMi HOG000252054.
    KOi K04094.
    OMAi WEMRAAG.
    OrthoDBi EOG6J74VT.

    Enzyme and pathway databases

    BioCyci SMED366394:GJAL-1198-MONOMER.

    Family and domain databases

    HAMAPi MF_01037. TrmFO.
    InterProi IPR004417. Folate-dep_Ribothymidyl_synth.
    IPR002218. GIDA-rel.
    IPR020595. GIDA-rel_CS.
    [Graphical view ]
    Pfami PF01134. GIDA. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00137. gid_trmFO. 1 hit.
    PROSITEi PS01281. GIDA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of Sinorhizobium medicae WSM419 chromosome."
      US DOE Joint Genome Institute
      Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Reeve W.G., Richardson P.
      Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: WSM419.

    Entry informationi

    Entry nameiTRMFO_SINMW
    AccessioniPrimary (citable) accession number: A6U8P9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: August 21, 2007
    Last modified: May 14, 2014
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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