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A6U8P9 (TRMFO_SINMW) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO
EC=2.1.1.74
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferase
Folate-dependent tRNA(M-5-U54)-methyltransferase
Gene names
Name:trmFO
Synonyms:gid
Ordered Locus Names:Smed_1178
OrganismSinorhizobium medicae (strain WSM419) (Ensifer medicae) [Complete proteome] [HAMAP]
Taxonomic identifier366394 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium ›

Protein attributes

Sequence length480 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs By similarity. HAMAP-Rule MF_01037

Catalytic activity

5,10-methylenetetrahydrofolate + uridine54 in tRNA + FADH2 = tetrahydrofolate + 5-methyluridine54 in tRNA + FAD. HAMAP-Rule MF_01037

Cofactor

FAD By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the MnmG family. TrmFO subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 480480Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO HAMAP-Rule MF_01037
PRO_1000063928

Regions

Nucleotide binding15 – 206FAD By similarity

Sequences

Sequence LengthMass (Da)Tools
A6U8P9 [UniParc].

Last modified August 21, 2007. Version 1.
Checksum: AA6A7587022C1B28

FASTA48051,129
        10         20         30         40         50         60 
MNKLTSSYSP VHVIGGGLAG SEAAWQIAEA GVPVILHEMR GVRGTDAHKT DSLAELVCSN 

        70         80         90        100        110        120 
SFRSDDATSN AVGVLHAEMR LAGSIIMRCA DLNQVPAGGA LAVDREGFAE AVSAAIAAHP 

       130        140        150        160        170        180 
LITVLREEIR GLPPKEWDLA IIATGPLTAP DLADAIRAET GADALAFFDA IAPIVHADTI 

       190        200        210        220        230        240 
DMDICWHQSR YDKVGPGGTG KDYINCPLTQ EEYNAFIDAL IAGDKTGFKE WEGTPYFDGC 

       250        260        270        280        290        300 
LPIEVMAERG RETLRHGPMK PMGLTNAHNP SVKPYAVVQL RQDNALGTLY NMVGFQTKLK 

       310        320        330        340        350        360 
YGAQGEVFRM IPGLEKAEFA RLGGLHRNTY INSPTLLDQS LTLKSRAGLR FAGQITGCEG 

       370        380        390        400        410        420 
YVESASIGLL AGRFAAAERK GASPAIPPVT TAFGALLNHI TGGHIVSDDE PGKRSFQPMN 

       430        440        450        460        470        480 
VNFGLFPPLD PGALTRPEGA KRFRGKEKAS AKKQAMASRA LSDCATWLDK NLLIGGAATR

« Hide

References

[1]"Complete sequence of Sinorhizobium medicae WSM419 chromosome."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Reeve W.G., Richardson P.
Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: WSM419.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000738 Genomic DNA. Translation: ABR60029.1.
RefSeqYP_001326864.1. NC_009636.1.

3D structure databases

ProteinModelPortalA6U8P9.
ModBaseSearch...

Protein-protein interaction databases

STRING366394.Smed_1178.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABR60029; ABR60029; Smed_1178.
GeneID5322024.
KEGGsmd:Smed_1178.
PATRIC23621306. VBISinMed134228_4305.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1206.
HOGENOMHOG000252054.
KOK04094.
OMARFAGQIT.
ProtClustDBPRK05335.

Enzyme and pathway databases

BioCycSMED366394:GJAL-1198-MONOMER.

Family and domain databases

HAMAPMF_01037. TrmFO.
InterProIPR004417. Folate-dep_Ribothymidyl_synth.
IPR002218. GIDA-rel.
IPR020595. GIDA-rel_CS.
[Graphical view]
PfamPF01134. GIDA. 1 hit.
[Graphical view]
TIGRFAMsTIGR00137. gid_trmFO. 1 hit.
PROSITEPS01280. GIDA_1. False negative.
PS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMFO_SINMW
AccessionPrimary (citable) accession number: A6U8P9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: August 21, 2007
Last modified: May 1, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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