Reviewed, UniProtKB/Swiss-Prot A6U6Y8 (BETA_SINMW)

Names and origin

Protein names

Recommended name:
    Choline dehydrogenase
      Short name=CHD
      Short name=CDH
    EC=1.1.99.1

Gene names

Name:	betA
Ordered Locus Names:	Smed_0562

Organism

Sinorhizobium medicae (strain WSM419) (Ensifer medicae) [Complete proteome] [HAMAP]

Taxonomic identifier

366394 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	549 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Can catalyze the oxidation of choline to betaine aldehyde and betaine aldehyde to glycine betaine By similarity.
Catalytic activity	Choline + acceptor = betaine aldehyde + reduced acceptor. HAMAP MF_00750
Cofactor	FAD By similarity.
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (cytochrome c reductase route): step 1/1. HAMAP MF_00750
Sequence similarities	Belongs to the GMC oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine betaine biosynthetic process from choline Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro choline dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 549	549	Choline dehydrogenase HAMAP MF_00750		PRO_1000046572
Regions
	Nucleotide binding	4 – 33	30	FAD Probable
Sites
	Active site	465	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6U6Y8-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 97AF35082E32BEB7	FASTA	549	61,179
	10 20 30 40 50 60 MQADFVIIGS GSAGSALAYR LSEDGANSVV VLEFGGSDVG PFIQMPAALA WPMSMNRYNW 70 80 90 100 110 120 GYLSEPEPNL NNRRITAPRG KVIGGSSSIN GMVYVRGHSE DFDRWEELGA KGWAYADVLP 130 140 150 160 170 180 YYKRMEHSHG GEEGWRGTDG PLHVQRGPVK NPLFHAFIEA GKQAGFEVTE DYNGSKQEGF 190 200 210 220 230 240 GLMEQTTWRG RRWSAASAYL RPALKRPNVE LVRCFARKIV IENGRATGVE IERGGRTEVV 250 260 270 280 290 300 RANREVIVSA SSFNSPKLLM LSGIGPAAHL QEMGIDVKAD RPGVGQNLQD HMEFYFQQVS 310 320 330 340 350 360 TKPVSLYSWL PWFWQGVAGA QWLFFKRGLG ISNQFESCAF LRSAPGVKQP DIQYHFLPVA 370 380 390 400 410 420 ISYDGKAAAK SHGFQVHVGY NLSKSRGDVT LRSSDPKADP VIRFNYMSHP EDWEKFRHCV 430 440 450 460 470 480 RLTREIFGQK AFDLYRGPEI QPGEKVRTDE EIDAFLREHL ESAYHPCGTC KMGAKDDPMA 490 500 510 520 530 540 VVDPETRVIG VDGLRVADSS IFPHITYGNL NAPSIMTGEK AADHILGKQP LARSNQEPWI NPRWAVSDR

References

[1]

"Complete sequence of Sinorhizobium medicae WSM419 chromosome." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Reeve W.G., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000738 Genomic DNA. Translation: ABR59418.1.
RefSeq	YP_001326253.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6U6Y8.
Genome annotation databases
GeneID	5321398.
GenomeReviews	Gene locus Smed_0562 in contig CP000738_GR.
KEGG	smd:Smed_0562.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	HMTVKNG.
Family and domain databases
HAMAP	MF_00750. [Tree]
InterPro	IPR011533. Choline_dehydrogenase. IPR012132. GMC_OxRdtase. IPR000172. GMC_OxRdtase_N. IPR007867. GMC_OxRtase_C. [Graphical view]
Pfam	PF05199. GMC_oxred_C. 1 hit. PF00732. GMC_oxred_N. 1 hit. [Graphical view]
PIRSF	PIRSF000137. Alcohol_oxidase. 1 hit.
TIGRFAMs	TIGR01810. betA. 1 hit.
PROSITE	PS00623. GMC_OXRED_1. 1 hit. PS00624. GMC_OXRED_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BETA_SINMW

Accession

Primary (citable) accession number: A6U6Y8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 21, 2007
Last modified:	November 3, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)