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A6U5H7

- TRMB_SINMW

UniProt

A6U5H7 - TRMB_SINMW

Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Sinorhizobium medicae (strain WSM419) (Ensifer medicae)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 45 (01 Oct 2014)
      Sequence version 1 (21 Aug 2007)
      Previous versions | rss
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    • Comment

    Functioni

    Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei63 – 631S-adenosyl-L-methionineUniRule annotation
    Binding sitei88 – 881S-adenosyl-L-methionineUniRule annotation
    Binding sitei115 – 1151S-adenosyl-L-methionineUniRule annotation
    Active sitei137 – 1371By similarity
    Binding sitei137 – 1371S-adenosyl-L-methionineUniRule annotation
    Binding sitei141 – 1411SubstrateUniRule annotation
    Binding sitei173 – 1731SubstrateUniRule annotation

    GO - Molecular functioni

    1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciSMED366394:GJAL-47-MONOMER.
    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
    tRNA(m7G46)-methyltransferaseUniRule annotation
    Gene namesi
    Name:trmBUniRule annotation
    Ordered Locus Names:Smed_0047
    OrganismiSinorhizobium medicae (strain WSM419) (Ensifer medicae)
    Taxonomic identifieri366394 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
    ProteomesiUP000001108: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 232232tRNA (guanine-N(7)-)-methyltransferasePRO_1000064411Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi366394.Smed_0047.

    Structurei

    3D structure databases

    ProteinModelPortaliA6U5H7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni211 – 2144Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0220.
    HOGENOMiHOG000073969.
    KOiK03439.
    OMAiRAFYGRR.
    OrthoDBiEOG6K6VBC.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_01057. tRNA_methyltr_TrmB.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A6U5H7-1 [UniParc]FASTAAdd to Basket

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    MTETRGARAT EAFFGRRKGK PLRERQAAHL ENLLPELKID LEHAAPADLK    50
    ELFPVSVERV RLEIGFGGGE HLIHRAAEDP ATGFIGVEPF VNSMAKLLGQ 100
    IEAKAIRNIR LHDDDATQVL DWLPAASVDQ IDLLYPDPWP KRKHWKRRFV 150
    SEVNLDRFAR ILRPGGVFCF ASDIDSYINW TLIHCRDHAA FEWTAAGAAD 200
    WLAPFAGWPS TRYEAKARRE GRSSAYLSFR RS 232
    Length:232
    Mass (Da):26,344
    Last modified:August 21, 2007 - v1
    Checksum:iC77D52F4C7A47D47
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000738 Genomic DNA. Translation: ABR58907.1.
    RefSeqiYP_001325742.1. NC_009636.1.

    Genome annotation databases

    EnsemblBacteriaiABR58907; ABR58907; Smed_0047.
    GeneIDi5320874.
    KEGGismd:Smed_0047.
    PATRICi23618861. VBISinMed134228_3102.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000738 Genomic DNA. Translation: ABR58907.1 .
    RefSeqi YP_001325742.1. NC_009636.1.

    3D structure databases

    ProteinModelPortali A6U5H7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 366394.Smed_0047.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABR58907 ; ABR58907 ; Smed_0047 .
    GeneIDi 5320874.
    KEGGi smd:Smed_0047.
    PATRICi 23618861. VBISinMed134228_3102.

    Phylogenomic databases

    eggNOGi COG0220.
    HOGENOMi HOG000073969.
    KOi K03439.
    OMAi RAFYGRR.
    OrthoDBi EOG6K6VBC.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .
    BioCyci SMED366394:GJAL-47-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_01057. tRNA_methyltr_TrmB.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of Sinorhizobium medicae WSM419 chromosome."
      US DOE Joint Genome Institute
      Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Reeve W.G., Richardson P.
      Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: WSM419.

    Entry informationi

    Entry nameiTRMB_SINMW
    AccessioniPrimary (citable) accession number: A6U5H7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: August 21, 2007
    Last modified: October 1, 2014
    This is version 45 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3