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A6U5H7 (TRMB_SINMW) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:Smed_0047
OrganismSinorhizobium medicae (strain WSM419) (Ensifer medicae) [Complete proteome] [HAMAP]
Taxonomic identifier366394 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium

Protein attributes

Sequence length232 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 232232tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_1000064411

Regions

Region211 – 2144Substrate binding By similarity

Sites

Active site1371 By similarity
Binding site631S-adenosyl-L-methionine By similarity
Binding site881S-adenosyl-L-methionine By similarity
Binding site1151S-adenosyl-L-methionine By similarity
Binding site1371S-adenosyl-L-methionine By similarity
Binding site1411Substrate By similarity
Binding site1731Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
A6U5H7 [UniParc].

Last modified August 21, 2007. Version 1.
Checksum: C77D52F4C7A47D47

FASTA23226,344
        10         20         30         40         50         60 
MTETRGARAT EAFFGRRKGK PLRERQAAHL ENLLPELKID LEHAAPADLK ELFPVSVERV 

        70         80         90        100        110        120 
RLEIGFGGGE HLIHRAAEDP ATGFIGVEPF VNSMAKLLGQ IEAKAIRNIR LHDDDATQVL 

       130        140        150        160        170        180 
DWLPAASVDQ IDLLYPDPWP KRKHWKRRFV SEVNLDRFAR ILRPGGVFCF ASDIDSYINW 

       190        200        210        220        230 
TLIHCRDHAA FEWTAAGAAD WLAPFAGWPS TRYEAKARRE GRSSAYLSFR RS 

« Hide

References

[1]"Complete sequence of Sinorhizobium medicae WSM419 chromosome."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Reeve W.G., Richardson P.
Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: WSM419.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000738 Genomic DNA. Translation: ABR58907.1.
RefSeqYP_001325742.1. NC_009636.1.

3D structure databases

ProteinModelPortalA6U5H7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING366394.Smed_0047.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABR58907; ABR58907; Smed_0047.
GeneID5320874.
KEGGsmd:Smed_0047.
PATRIC23618861. VBISinMed134228_3102.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073969.
KOK03439.
OMAIGCEPFV.
OrthoDBEOG6K6VBC.
ProtClustDBPRK00121.

Enzyme and pathway databases

BioCycSMED366394:GJAL-47-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_SINMW
AccessionPrimary (citable) accession number: A6U5H7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: August 21, 2007
Last modified: April 16, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways