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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Sinorhizobium medicae (strain WSM419) (Ensifer medicae)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei63S-adenosyl-L-methionineUniRule annotation1
Binding sitei88S-adenosyl-L-methionineUniRule annotation1
Binding sitei115S-adenosyl-L-methionineUniRule annotation1
Active sitei137By similarity1
Binding sitei137S-adenosyl-L-methionineUniRule annotation1
Binding sitei141SubstrateUniRule annotation1
Binding sitei173SubstrateUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:Smed_0047
OrganismiSinorhizobium medicae (strain WSM419) (Ensifer medicae)
Taxonomic identifieri366394 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
Proteomesi
  • UP000001108 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000644111 – 232tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST232

Proteomic databases

PRIDEiA6U5H7

Interactioni

Protein-protein interaction databases

STRINGi366394.Smed_0047

Structurei

3D structure databases

ProteinModelPortaliA6U5H7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni211 – 214Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ1 Bacteria
COG0220 LUCA
HOGENOMiHOG000073969
KOiK03439
OMAiPDPWHKS
OrthoDBiPOG091H015P

Family and domain databases

HAMAPiMF_01057 tRNA_methyltr_TrmB, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

A6U5H7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTETRGARAT EAFFGRRKGK PLRERQAAHL ENLLPELKID LEHAAPADLK
60 70 80 90 100
ELFPVSVERV RLEIGFGGGE HLIHRAAEDP ATGFIGVEPF VNSMAKLLGQ
110 120 130 140 150
IEAKAIRNIR LHDDDATQVL DWLPAASVDQ IDLLYPDPWP KRKHWKRRFV
160 170 180 190 200
SEVNLDRFAR ILRPGGVFCF ASDIDSYINW TLIHCRDHAA FEWTAAGAAD
210 220 230
WLAPFAGWPS TRYEAKARRE GRSSAYLSFR RS
Length:232
Mass (Da):26,344
Last modified:August 21, 2007 - v1
Checksum:iC77D52F4C7A47D47
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000738 Genomic DNA Translation: ABR58907.1
RefSeqiWP_011974261.1, NC_009636.1
YP_001325742.1, NC_009636.1

Genome annotation databases

EnsemblBacteriaiABR58907; ABR58907; Smed_0047
GeneIDi5320874
KEGGismd:Smed_0047
PATRICifig|366394.8.peg.3102

Similar proteinsi

Entry informationi

Entry nameiTRMB_SINMW
AccessioniPrimary (citable) accession number: A6U5H7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: August 21, 2007
Last modified: May 23, 2018
This is version 56 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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