A6U5G1 (GLND_SINMW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
[Protein-PII] uridylyltransferase
Short name=PII uridylyl-transferase
EC=2.7.7.59

Alternative name(s):
UTase
Uridylyl-removing enzyme

Gene names

Name:	glnD
Ordered Locus Names:	Smed_0031

Organism

Sinorhizobium medicae (strain WSM419) (Ensifer medicae) [Complete proteome] [HAMAP]

Taxonomic identifier

366394 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium › Sinorhizobium medicae

Protein attributes

Sequence length	949 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Modifies, by uridylylation or deuridylylation the PII (GlnB) regulatory protein By similarity. HAMAP-Rule MF_00277
Catalytic activity	UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII]. HAMAP-Rule MF_00277
Sequence similarities	Belongs to the GlnD family.

Ontologies

Keywords
Biological process	Nitrogen fixation
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	nitrogen fixation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	[protein-PII] uridylyltransferase activity Inferred from electronic annotation. Source: EC amino acid binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: InterPro phosphoric diester hydrolase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 949	949	[Protein-PII] uridylyltransferase HAMAP-Rule MF_00277		PRO_1000022354

Sequences

	Sequence		Length	Mass (Da)
	A6U5G1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: E18E0283457CC20C	FASTA	949	106,228
	10 20 30 40 50 60 MARHETSFPE ILDVAALRAR CDFIASAHAE QREPMRRALL AAFKEANIAG RAKARELLAA 70 80 90 100 110 120 DGAGIKCAER ISWLQDQLIT LLHDFVLNQV FDAAKAPETS RIAVTAVGGY GRGTLAPGSD 130 140 150 160 170 180 IDLLFLLPAK KAVWAEPAIE FMLYILWDLG FKVGHATRTI EDCIRLSRAD MTIRTAILEC 190 200 210 220 230 240 RYVCGSVALA SELETRFDHE IVRNTGPEFI AAKLAERDER HRKAGDTRYL VEPNVKEGKG 250 260 270 280 290 300 GLRDLHTLFW ISKYFYRVKD SADLVKLGVL SRQEYKLFQK AEDFLWAVRC HMHFLTGKAE 310 320 330 340 350 360 ERLSFDIQRE IAEALGYHDH PGLSAVERFM KHYFLVAKDV GDLTRIFCSA LEDQQAKDAP 370 380 390 400 410 420 GISGVISRFR NRVRKIPGTL DFVDDGGRIA LASPDVFKRD PVNLLRMFHI ADINGLEFHP 430 440 450 460 470 480 AALKQVTRSL SLITPHLREN EEANRLFLSI LTSRRNPELI LRRMNEAAVL GRFIPEFGKI 490 500 510 520 530 540 VSMMQFNMYH HYTVDEHLLR AVDVLSRIER GLEEEAHPLT AMLMPAIEDR EALYVAVLLH 550 560 570 580 590 600 DIAKGRPEDH SVAGAKVARK LCPRFRLSPK QTETVVWLVE EHLTMSMVAQ TRDLNDRKTI 610 620 630 640 650 660 VDFAERVQSL ERLKMLLILT VCDIRAVGPG VWNGWKGQLL RTLYYETELL LSGGFSELSR 670 680 690 700 710 720 KERAKHAADM LEEALADWPK EERQTYVRLH YQPYLLTVAL DEQVRHAAFI READAAGRTL 730 740 750 760 770 780 ATMVRTHDFH AITEITVLSP DHPRLLTVIA GACAAAGANI VGAQIHTTSD GRALDTILVN 790 800 810 820 830 840 REFSVAEDET RRAASIGKLI EDVLSGRKKL PDVIASRTRS KKRSRAFTVT PEVTISNALS 850 860 870 880 890 900 NKFTVIEVEG LDRTGLLSEV TAVLSDLSLD IASAHITTFG EKVIDTFYVT DLVGSKITSE 910 920 930 940 NRQMNIAARL KAVLAGEVDE ARERMPSGII APTPVPRASH GSKATKAET

References

[1]

"Complete sequence of Sinorhizobium medicae WSM419 chromosome." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Reeve W.G., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: WSM419.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000738 Genomic DNA. Translation: ABR58891.1.
RefSeq	YP_001325726.1. NC_009636.1.
3D structure databases
ProteinModelPortal	A6U5G1.
ModBase	Search...
Protein-protein interaction databases
STRING	366394.Smed_0031.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABR58891; ABR58891; Smed_0031.
GeneID	5320858.
KEGG	smd:Smed_0031.
PATRIC	23618827. VBISinMed134228_3085.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2844.
HOGENOM	HOG000261779.
KO	K00990.
OMA	SPKVWNA.
ProtClustDB	PRK05092.
Enzyme and pathway databases
BioCyc	SMED366394:GJAL-31-MONOMER.
Family and domain databases
Gene3D	1.10.3210.10. 1 hit.
HAMAP	MF_00277. PII_uridylyl-transf.
InterPro	IPR002912. ACT_dom. IPR010043. GlnD_Uridyltrans. IPR003607. HD/PDEase_dom. IPR006674. HD_domain. IPR002934. Nucleotidyltransferase. IPR013546. PII_UdlTrfase/GS_AdlTrfase. [Graphical view]
PANTHER	PTHR13734:SF1. PTHR13734:SF1. 1 hit.
Pfam	PF01842. ACT. 2 hits. PF08335. GlnD_UR_UTase. 1 hit. PF01966. HD. 1 hit. PF01909. NTP_transf_2. 1 hit. [Graphical view]
PIRSF	PIRSF006288. PII_uridyltransf. 1 hit.
SMART	SM00471. HDc. 1 hit. [Graphical view]
TIGRFAMs	TIGR01693. UTase_glnD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLND_SINMW

Accession

Primary (citable) accession number: A6U5G1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 21, 2007
Last modified:	May 29, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program