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A6U4F5 (BIOB_STAA2) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:SaurJH1_2499
OrganismStaphylococcus aureus (strain JH1) [Complete proteome] [HAMAP]
Taxonomic identifier359787 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length335 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 335335Biotin synthase HAMAP-Rule MF_01694
PRO_0000381643

Sites

Metal binding611Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding651Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding681Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1041Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1371Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1971Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2671Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A6U4F5 [UniParc].

Last modified August 21, 2007. Version 1.
Checksum: 0C7DAB4ECCB73A5B

FASTA33537,545
        10         20         30         40         50         60 
MNLAKRILQG EQLTKETVLK IYEDTNIDTL DLLNEAYILR KHYFGKKVKL NMILNAKSGI 

        70         80         90        100        110        120 
CPENCGYCGQ SRDIKQKQRY ALIPEEQIID GAKVAHDNHI GTYCIVMSGR GPSDKEVDHI 

       130        140        150        160        170        180 
SNTVRTIKSQ HPQLKICACL GLTNDEQAKK LKSAGVDRYN HNINTSENYH DNVVTTHSYK 

       190        200        210        220        230        240 
DRTDTIELMK ANNISPCSGV ICGMGESNQD IVDMAFALKE MDADSIPINF LHPIKGTKFG 

       250        260        270        280        290        300 
SMDDLTPMKC LRIVALFRLI NPTKEIRIAG GREVNLRSLQ PLALKAANSI FVGDYLITGG 

       310        320        330 
QPNQLDYDMI NDLGFEIDYD TCENKENKND VSRAN 

« Hide

References

[1]"Complete sequence of chromosome of Staphylococcus aureus subsp. aureus JH1."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Ivanova N., Tomasz A., Richardson P.
Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JH1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000736 Genomic DNA. Translation: ABR53323.1.
RefSeqYP_001317610.1. NC_009632.1.

3D structure databases

ProteinModelPortalA6U4F5.
SMRA6U4F5. Positions 10-319.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING359787.SaurJH1_2499.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABR53323; ABR53323; SaurJH1_2499.
GeneID5315714.
KEGGsah:SaurJH1_2499.
PATRIC19536990. VBIStaAur98826_2553.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OMAADRFCMG.
OrthoDBEOG622PMP.
ProtClustDBPRK06256.

Enzyme and pathway databases

BioCycSAUR359787:GCG4-2577-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_STAA2
AccessionPrimary (citable) accession number: A6U4F5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: August 21, 2007
Last modified: February 19, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways