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Protein

DNA polymerase IV

Gene

dinB

Organism
Staphylococcus aureus (strain JH1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII.UniRule annotation

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi10MagnesiumUniRule annotation1
Sitei15Substrate discriminationUniRule annotation1
Metal bindingi105MagnesiumUniRule annotation1
Active sitei106UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Mutator protein, Nucleotidyltransferase, Transferase
Biological processDNA damage, DNA repair, DNA replication
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase IVUniRule annotation (EC:2.7.7.7UniRule annotation)
Short name:
Pol IVUniRule annotation
Gene namesi
Name:dinBUniRule annotation
Ordered Locus Names:SaurJH1_1983
OrganismiStaphylococcus aureus (strain JH1)
Taxonomic identifieri359787 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000849441 – 356DNA polymerase IVAdd BLAST356

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA6U2Z9
SMRiA6U2Z9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 187UmuCUniRule annotationAdd BLAST182

Sequence similaritiesi

Belongs to the DNA polymerase type-Y family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000082707
KOiK02346
OMAiGMQIREI

Family and domain databases

CDDicd03586 PolY_Pol_IV_kappa, 1 hit
Gene3Di3.30.1490.100, 1 hit
HAMAPiMF_01113 DNApol_IV, 1 hit
InterProiView protein in InterPro
IPR036775 DNA_pol_Y-fam_lit_finger_sf
IPR017961 DNA_pol_Y-fam_little_finger
IPR022880 DNApol_IV
IPR001126 UmuC
PfamiView protein in Pfam
PF00817 IMS, 1 hit
PF11799 IMS_C, 1 hit
SUPFAMiSSF100879 SSF100879, 1 hit
PROSITEiView protein in PROSITE
PS50173 UMUC, 1 hit

Sequencei

Sequence statusi: Complete.

A6U2Z9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTERRIIHID MDYFFAQVEM RDNPKLKGKP VIVGGKASSR GVVSTASYEA
60 70 80 90 100
RKYGVHSAMP MSQAHKLCPN GYFVTSNFGA YRETSAQIMS IFRSYTDKVE
110 120 130 140 150
PMSLDEAYLD ITELVRPDLP ASKIAQYIRK DILEQTHLTA SAGVSYNKFL
160 170 180 190 200
AKLASGMNKP DGLTVIDYQN VHDILMTLDI GDFPGVGKAS KKVMHDNGIF
210 220 230 240 250
NGRDLYEKTE FELIRLFGKR GRGLYNKARG IDHSEVKSSR VRKSVGTERT
260 270 280 290 300
FATDVNDDEE ILRKVWELSG KTAERLNKLQ KSAKTVTVKI KTYQFETLSK
310 320 330 340 350
QMSLRDSVSS EEDIYNIAYL LYNDLKDPDV PIRLIGVTVG NLEQSTNKNM

TIYDFI
Length:356
Mass (Da):40,248
Last modified:August 21, 2007 - v1
Checksum:iAFF7B14384825272
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000736 Genomic DNA Translation: ABR52817.1
RefSeqiWP_000140171.1, NC_009632.1

Genome annotation databases

EnsemblBacteriaiABR52817; ABR52817; SaurJH1_1983
KEGGisah:SaurJH1_1983

Similar proteinsi

Entry informationi

Entry nameiDPO4_STAA2
AccessioniPrimary (citable) accession number: A6U2Z9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: August 21, 2007
Last modified: March 28, 2018
This is version 69 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

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