A6U2J5 (SYY_STAA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine--tRNA ligase
EC=6.1.1.1

Alternative name(s):
Tyrosyl-tRNA synthetase
Short name=TyrRS

Gene names

Name:	tyrS
Ordered Locus Names:	SaurJH1_1819

Organism

Staphylococcus aureus (strain JH1) [Complete proteome] [HAMAP]

Taxonomic identifier

359787 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus aureus › Staphylococcus aureus subsp. aureus

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity. HAMAP-Rule MF_02006
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). HAMAP-Rule MF_02006
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP RNA binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 420	420	Tyrosine--tRNA ligase HAMAP-Rule MF_02006		PRO_1000088627
Regions
	Domain	353 – 420	68	S4 RNA-binding
	Motif	41 – 50	10	"HIGH" region HAMAP-Rule MF_02006
	Motif	231 – 235	5	"KMSKS" region HAMAP-Rule MF_02006
Sites
	Binding site	36	1	Tyrosine By similarity
	Binding site	170	1	Tyrosine By similarity
	Binding site	174	1	Tyrosine By similarity
	Binding site	234	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6U2J5 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: B6916EA4BE4B21B2	FASTA	420	47,598
	10 20 30 40 50 60 MTNVLIEDLK WRGLIYQQTD EQGIEDLLNK EQVTLYCGAD PTADSLHIGH LLPFLTLRRF 70 80 90 100 110 120 QEHGHRPIVL IGGGTGMIGD PSGKSEERVL QTEEQVDKNI EGISKQMHNI FEFGTDHGAV 130 140 150 160 170 180 LVNNRDWLGQ ISLISFLRDY GKHVGVNYML GKDSIQSRLE HGISYTEFTY TILQAIDFGH 190 200 210 220 230 240 LNRELNCKIQ VGGSDQWGNI TSGIELMRRM YGQTDAYGLT IPLVTKSDGK KFGKSESGAV 250 260 270 280 290 300 WLDAEKTSPY EFYQFWINQS DEDVIKFLKY FTFLGKEEID RLEQSKNEAP HLREAQKTLA 310 320 330 340 350 360 EEVTKFIHGE DALNDAIRIS QALFSGDLKS LSAKELKDGF KDVPQVTLSN DTTNIVEVLI 370 380 390 400 410 420 ETGISPSKRQ AREDVNNGAI YINGERQQDV NYALAPEDKI DGEFTIIRRG KKKYFMVNYQ

References

[1]

"Complete sequence of chromosome of Staphylococcus aureus subsp. aureus JH1." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Ivanova N., Tomasz A., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JH1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000736 Genomic DNA. Translation: ABR52663.1.
RefSeq	YP_001316950.1. NC_009632.1.
3D structure databases
ProteinModelPortal	A6U2J5.
SMR	A6U2J5. Positions 2-419.
ModBase	Search...
Protein-protein interaction databases
STRING	359787.SaurJH1_1819.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABR52663; ABR52663; SaurJH1_1819.
GeneID	5316809.
KEGG	sah:SaurJH1_1819.
PATRIC	19535504. VBIStaAur98826_1862.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0162.
HOGENOM	HOG000242790.
KO	K01866.
OMA	GKKKHVL.
ProtClustDB	PRK05912.
Family and domain databases
Gene3D	3.10.290.10. 1 hit. 3.40.50.620. 1 hit.
HAMAP	MF_02006. Tyr_tRNA_synth_type1.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. IPR002942. S4_RNA-bd. IPR002307. Tyr-tRNA-ligase. IPR024088. Tyr-tRNA-ligase_bac-type. IPR024107. Tyr-tRNA-ligase_bac_1. [Graphical view]
PANTHER	PTHR11766. PTHR11766. 1 hit.
Pfam	PF01479. S4. 1 hit. PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01040. TRNASYNTHTYR.
SMART	SM00363. S4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00234. tyrS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	A6U2J5.

Entry information

Entry name

SYY_STAA2

Accession

Primary (citable) accession number: A6U2J5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	August 21, 2007
Last modified:	May 1, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program