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Protein

tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase

Gene

miaB

Organism
Staphylococcus aureus (strain JH1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine.UniRule annotation

Catalytic activityi

N6-dimethylallyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + reduced electron acceptor = 2-methylthio-N6-dimethylallyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + electron acceptor.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi77Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi113Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi147Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi223Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi227Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi230Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processtRNA processing
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseUniRule annotation (EC:2.8.4.3UniRule annotation)
Alternative name(s):
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaBUniRule annotation
tRNA-i(6)A37 methylthiotransferaseUniRule annotation
Gene namesi
Name:miaBUniRule annotation
Ordered Locus Names:SaurJH1_1378
OrganismiStaphylococcus aureus (strain JH1)
Taxonomic identifieri359787 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003745661 – 514tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseAdd BLAST514

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA6U1B1
SMRiA6U1B1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini68 – 186MTTase N-terminalUniRule annotationAdd BLAST119
Domaini442 – 505TRAMUniRule annotationAdd BLAST64

Sequence similaritiesi

Belongs to the methylthiotransferase family. MiaB subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000224767
KOiK06168
OMAiKVCEHFH

Family and domain databases

Gene3Di3.40.50.12160, 1 hit
3.80.30.20, 1 hit
HAMAPiMF_01864 tRNA_metthiotr_MiaB, 1 hit
InterProiView protein in InterPro
IPR006638 Elp3/MiaB/NifB
IPR005839 Methylthiotransferase
IPR020612 Methylthiotransferase_CS
IPR013848 Methylthiotransferase_N
IPR038135 Methylthiotransferase_N_sf
IPR006463 MiaB_methiolase
IPR007197 rSAM
IPR023404 rSAM_horseshoe
IPR002792 TRAM_dom
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PF01938 TRAM, 1 hit
PF00919 UPF0004, 1 hit
SFLDiSFLDF00273 (dimethylallyl)adenosine_tRNA_, 1 hit
SFLDG01061 methylthiotransferase, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00089 TIGR00089, 1 hit
PROSITEiView protein in PROSITE
PS51449 MTTASE_N, 1 hit
PS01278 MTTASE_RADICAL, 1 hit
PS50926 TRAM, 1 hit

Sequencei

Sequence statusi: Complete.

A6U1B1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNEEQRKASS VDVLAERDKK AEKDYSKYFE HVYQPPNLKE AKKRGKQEVR
60 70 80 90 100
YNRDFQIDEK YRGMGNERTF LIKTYGCQMN AHDTEVIAGI LEALGYQATT
110 120 130 140 150
DINTADVILI NTCAIRENAE NKVFSEIGNL KHLKKERPDI LIGVCGCMSQ
160 170 180 190 200
EESVVNKILK SYQNVDMIFG THNIHHLPEI LEEAYLSKAM VVEVWSKEGD
210 220 230 240 250
VIENLPKVRE GNIKAWVNIM YGCDKFCTYC IVPFTRGKER SRRPEDIIDE
260 270 280 290 300
VRELAREGYK EITLLGQNVN SYGKDLQDIE YDLGDLLQAI SKIAIPRVRF
310 320 330 340 350
TTSHPWDFTD HMIDVISEGG NIVPHIHLPV QSGNNAVLKI MGRKYTRESY
360 370 380 390 400
LDLVKRIKDR IPNVALTTDI IVGYPNESEE QFEETLTLYD EVGFEHAYTY
410 420 430 440 450
LYSQRDGTPA AKMKDNVPLN VKKERLQRLN KKVGHYSQIA MSKYEGQTVT
460 470 480 490 500
VLCEGSSKKD DQVLAGYTDK NKLVNFKAPK EMIGKLVEVR IDEAKQYSLN
510
GSFVKEVEPE MVIQ
Length:514
Mass (Da):58,917
Last modified:August 21, 2007 - v1
Checksum:i9E864C7A17B4EAD8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000736 Genomic DNA Translation: ABR52229.1
RefSeqiWP_001001524.1, NC_009632.1

Genome annotation databases

EnsemblBacteriaiABR52229; ABR52229; SaurJH1_1378
KEGGisah:SaurJH1_1378

Similar proteinsi

Entry informationi

Entry nameiMIAB_STAA2
AccessioniPrimary (citable) accession number: A6U1B1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: August 21, 2007
Last modified: March 28, 2018
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health