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Protein

tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase

Gene

miaB

Organism
Staphylococcus aureus (strain JH1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine.UniRule annotation

Catalytic activityi

N(6)-dimethylallyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + reduced electron acceptor = 2-methylthio-N(6)-dimethylallyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + electron acceptor.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi77 – 771Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi113 – 1131Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi147 – 1471Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi223 – 2231Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi227 – 2271Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi230 – 2301Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSAUR359787:GCG4-1404-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseUniRule annotation (EC:2.8.4.3UniRule annotation)
Alternative name(s):
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaBUniRule annotation
tRNA-i(6)A37 methylthiotransferaseUniRule annotation
Gene namesi
Name:miaBUniRule annotation
Ordered Locus Names:SaurJH1_1378
OrganismiStaphylococcus aureus (strain JH1)
Taxonomic identifieri359787 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 514514tRNA-2-methylthio-N(6)-dimethylallyladenosine synthasePRO_0000374566Add
BLAST

Proteomic databases

PRIDEiA6U1B1.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA6U1B1.
SMRiA6U1B1. Positions 229-504.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini68 – 186119MTTase N-terminalUniRule annotationAdd
BLAST
Domaini442 – 50564TRAMUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the methylthiotransferase family. MiaB subfamily.UniRule annotation
Contains 1 MTTase N-terminal domain.UniRule annotation
Contains 1 TRAM domain.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000224767.
KOiK06168.
OMAiCQMNFSD.
OrthoDBiEOG6P5ZD8.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
HAMAPiMF_01864. tRNA_metthiotr_MiaB.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6U1B1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNEEQRKASS VDVLAERDKK AEKDYSKYFE HVYQPPNLKE AKKRGKQEVR
60 70 80 90 100
YNRDFQIDEK YRGMGNERTF LIKTYGCQMN AHDTEVIAGI LEALGYQATT
110 120 130 140 150
DINTADVILI NTCAIRENAE NKVFSEIGNL KHLKKERPDI LIGVCGCMSQ
160 170 180 190 200
EESVVNKILK SYQNVDMIFG THNIHHLPEI LEEAYLSKAM VVEVWSKEGD
210 220 230 240 250
VIENLPKVRE GNIKAWVNIM YGCDKFCTYC IVPFTRGKER SRRPEDIIDE
260 270 280 290 300
VRELAREGYK EITLLGQNVN SYGKDLQDIE YDLGDLLQAI SKIAIPRVRF
310 320 330 340 350
TTSHPWDFTD HMIDVISEGG NIVPHIHLPV QSGNNAVLKI MGRKYTRESY
360 370 380 390 400
LDLVKRIKDR IPNVALTTDI IVGYPNESEE QFEETLTLYD EVGFEHAYTY
410 420 430 440 450
LYSQRDGTPA AKMKDNVPLN VKKERLQRLN KKVGHYSQIA MSKYEGQTVT
460 470 480 490 500
VLCEGSSKKD DQVLAGYTDK NKLVNFKAPK EMIGKLVEVR IDEAKQYSLN
510
GSFVKEVEPE MVIQ
Length:514
Mass (Da):58,917
Last modified:August 21, 2007 - v1
Checksum:i9E864C7A17B4EAD8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000736 Genomic DNA. Translation: ABR52229.1.
RefSeqiWP_001001524.1. NC_009632.1.

Genome annotation databases

EnsemblBacteriaiABR52229; ABR52229; SaurJH1_1378.
KEGGisah:SaurJH1_1378.
PATRICi19534606. VBIStaAur98826_1412.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000736 Genomic DNA. Translation: ABR52229.1.
RefSeqiWP_001001524.1. NC_009632.1.

3D structure databases

ProteinModelPortaliA6U1B1.
SMRiA6U1B1. Positions 229-504.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiA6U1B1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABR52229; ABR52229; SaurJH1_1378.
KEGGisah:SaurJH1_1378.
PATRICi19534606. VBIStaAur98826_1412.

Phylogenomic databases

HOGENOMiHOG000224767.
KOiK06168.
OMAiCQMNFSD.
OrthoDBiEOG6P5ZD8.

Enzyme and pathway databases

BioCyciSAUR359787:GCG4-1404-MONOMER.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
HAMAPiMF_01864. tRNA_metthiotr_MiaB.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome of Staphylococcus aureus subsp. aureus JH1."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.
    , Hauser L., Kyrpides N., Ivanova N., Tomasz A., Richardson P.
    Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JH1.

Entry informationi

Entry nameiMIAB_STAA2
AccessioniPrimary (citable) accession number: A6U1B1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: August 21, 2007
Last modified: March 16, 2016
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.