A6U0V5 (SYFA_STAA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase alpha chain
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase alpha chain
Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	SaurJH1_1219

Organism

Staphylococcus aureus (strain JH1) [Complete proteome] [HAMAP]

Taxonomic identifier

359787 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	352 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00281
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_00281.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 352	352	Phenylalanine--tRNA ligase alpha chain HAMAP MF_00281		PRO_1000078851
Sites
	Metal binding	258	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6U0V5 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 01FD437729B8FDF7	FASTA	352	40,121
	10 20 30 40 50 60 MSEQQTMSEL KQQALVDINE ANDERALQEV KVKYLGKKGS VSGLMKLMKD LPNEEKPAFG 70 80 90 100 110 120 QKVNELRQTI QNELDERQQM LVKEKLNKQL AEETIDVSLP GRHIEIGSKH PLTRTIEEIE 130 140 150 160 170 180 DLFLGLGYEI VNGYEVEQDH YNFEMLNLPK SHPARDMQDS FYITDEILLR THTSPVQART 190 200 210 220 230 240 MESRHGQGPV KIICPGKVYR RDSDDATHSH QFTQIEGLVV DKNVKMSDLK GTLELLAKKL 250 260 270 280 290 300 FGADREIRLR PSYFPFTEPS VEVDVSCFKC KGKGCNVCKH TGWIEILGAG MVHPNVLEMA 310 320 330 340 350 GFDSSEYSGF AFGMGPDRIA MLKYGIEDIR HFYTNDVRFL DQFKAVEDRG DM

References

[1]

"Complete sequence of chromosome of Staphylococcus aureus subsp. aureus JH1." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Ivanova N., Tomasz A., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JH1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000736 Genomic DNA. Translation: ABR52073.1.
RefSeq	YP_001316360.1. NC_009632.1.
3D structure databases
ProteinModelPortal	A6U0V5.
SMR	A6U0V5. Positions 84-351.
ModBase	Search...
Protein-protein interaction databases
STRING	A6U0V5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000014036; EBSTAP00000013539; EBSTAG00000014035.
GeneID	5316636.
GenomeReviews	Gene locus SaurJH1_1219 in contig CP000736_GR.
KEGG	sah:SaurJH1_1219.
PATRIC	19534285. VBIStaAur98826_1253.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0016.
GeneTree	EBGT00050000024011.
HOGENOM	HBG284353.
OMA	FRASYFP.
ProtClustDB	PRK00488.
Enzyme and pathway databases
BioCyc	SAUR359787:SAURJH1_1219-MONOMER.
Family and domain databases
HAMAP	MF_00281. Phe_tRNA_synth_alpha1. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR022911. Phe_tRNA_synth_alpha1_bac. IPR002319. Phenylalanyl-tRNA_Synthase. IPR010978. tRNA-bd_arm. [Graphical view]
KO	K01889.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
SUPFAM	SSF46589. tRNA_binding_arm. 1 hit.
TIGRFAMs	TIGR00468. PheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	A6U0V5.

Entry information

Entry name

SYFA_STAA2

Accession

Primary (citable) accession number: A6U0V5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	August 21, 2007
Last modified:	January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program