Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Bifunctional protein FolD

Gene

folD

Organism
Staphylococcus aureus (strain JH1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH.UniRule annotation
5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate.UniRule annotation

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei190 – 1901NADPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi165 – 1673NADPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Histidine biosynthesis, Methionine biosynthesis, One-carbon metabolism, Purine biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciSAUR359787:GCG4-1171-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein FolDUniRule annotation
Including the following 2 domains:
Methylenetetrahydrofolate dehydrogenaseUniRule annotation (EC:1.5.1.5UniRule annotation)
Methenyltetrahydrofolate cyclohydrolaseUniRule annotation (EC:3.5.4.9UniRule annotation)
Gene namesi
Name:folDUniRule annotation
Ordered Locus Names:SaurJH1_1145
OrganismiStaphylococcus aureus (strain JH1)
Taxonomic identifieri359787 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 286286Bifunctional protein FolDPRO_1000087922Add
BLAST

Proteomic databases

PRIDEiA6U0N1.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA6U0N1.
SMRiA6U0N1. Positions 5-275.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000218242.
KOiK01491.
OMAiGQPMALM.
OrthoDBiEOG6K6VBB.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01576. THF_DHG_CYH.
InterProiIPR016040. NAD(P)-bd_dom.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view]
PfamiPF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

A6U0N1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVAKILDGKQ IAKDYRQGLQ DQVEALKEKG FTPKLSVILV GNDGASQSYV
60 70 80 90 100
RSKKKAAEKI GMISEIVHLE ETATEEEVLN ELNRLNNDDS VSGILVQVPL
110 120 130 140 150
PKQVSEQKIL EAINPEKDVD GFHPINIGKL YIDEQTFVPC TPLGIMEILK
160 170 180 190 200
HADIDLEAKN AVVIGRSHIV GQPVSKLLLQ KNASVTILHS RSKDMASYLK
210 220 230 240 250
DADVIVSAVG KPSLVTKDVV KEGAVIIDVG NTPDENGKLK GDVDYDAVKE
260 270 280
IAGAITPVPG GVGPLTITMV LNNTLLAEKM RRGIDS
Length:286
Mass (Da):30,888
Last modified:August 21, 2007 - v1
Checksum:i90A8356B96C4B741
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000736 Genomic DNA. Translation: ABR51999.1.
RefSeqiWP_000225836.1. NC_009632.1.

Genome annotation databases

EnsemblBacteriaiABR51999; ABR51999; SaurJH1_1145.
KEGGisah:SaurJH1_1145.
PATRICi19534131. VBIStaAur98826_1176.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000736 Genomic DNA. Translation: ABR51999.1.
RefSeqiWP_000225836.1. NC_009632.1.

3D structure databases

ProteinModelPortaliA6U0N1.
SMRiA6U0N1. Positions 5-275.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiA6U0N1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABR51999; ABR51999; SaurJH1_1145.
KEGGisah:SaurJH1_1145.
PATRICi19534131. VBIStaAur98826_1176.

Phylogenomic databases

HOGENOMiHOG000218242.
KOiK01491.
OMAiGQPMALM.
OrthoDBiEOG6K6VBB.

Enzyme and pathway databases

UniPathwayiUPA00193.
BioCyciSAUR359787:GCG4-1171-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01576. THF_DHG_CYH.
InterProiIPR016040. NAD(P)-bd_dom.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view]
PfamiPF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome of Staphylococcus aureus subsp. aureus JH1."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.
    , Hauser L., Kyrpides N., Ivanova N., Tomasz A., Richardson P.
    Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JH1.

Entry informationi

Entry nameiFOLD_STAA2
AccessioniPrimary (citable) accession number: A6U0N1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: August 21, 2007
Last modified: March 16, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.