A6TXX3 (PTG3C_STAA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
PTS system glucose-specific EIICBA component

Alternative name(s):
EIICBA-Glc
Short name=EII-Glc

Including the following 3 domains:

1. Glucose permease IIC component
   Alternative name(s):
   PTS system glucose-specific EIIC component
2. Glucose-specific phosphotransferase enzyme IIB component
   EC=2.7.1.69
   Alternative name(s):
   PTS system glucose-specific EIIB component
3. Glucose-specific phosphotransferase enzyme IIA component
   EC=2.7.1.-
   Alternative name(s):
   PTS system glucose-specific EIIA component

Gene names

Name:	ptsG
Synonyms:	glcA
Ordered Locus Names:	SaurJH1_0179

Organism

Staphylococcus aureus (strain JH1) [Complete proteome] [HAMAP]

Taxonomic identifier

359787 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	681 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in glucose transport By similarity.
Catalytic activity	Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate. Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Domain	The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site. The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain. The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.
Sequence similarities	Contains 1 PTS EIIA type-1 domain. Contains 1 PTS EIIB type-1 domain. Contains 1 PTS EIIC type-1 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glucose transmembrane transporter activity Inferred from electronic annotation. Source: InterPro kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 681	681	PTS system glucose-specific EIICBA component		PRO_5000256864
Regions
	Transmembrane	16 – 36	21	Helical; Potential
	Transmembrane	73 – 93	21	Helical; Potential
	Transmembrane	126 – 146	21	Helical; Potential
	Transmembrane	170 – 190	21	Helical; Potential
	Transmembrane	199 – 219	21	Helical; Potential
	Transmembrane	273 – 293	21	Helical; Potential
	Transmembrane	303 – 323	21	Helical; Potential
	Transmembrane	328 – 348	21	Helical; Potential
	Transmembrane	355 – 375	21	Helical; Potential
	Transmembrane	383 – 403	21	Helical; Potential
	Domain	3 – 414	412	PTS EIIC type-1
	Domain	425 – 506	82	PTS EIIB type-1
	Domain	551 – 655	105	PTS EIIA type-1
Sites
	Active site	447	1	Phosphocysteine intermediate; for EIIB activity By similarity
	Active site	603	1	Tele-phosphohistidine intermediate; for EIIA activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6TXX3 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 040ADA543CF31520	FASTA	681	73,958
	10 20 30 40 50 60 MRKKLFGQLQ RIGKALMLPV AILPAAGLLL AIGTAIQGEA LQHYLPFIQN GGVQNVAKLM 70 80 90 100 110 120 TAAGSIIFEN LPMIFALGVA IGLAGGDGVA AIAAFVGYII MNKTMGDFLQ VTPKNVTDPA 130 140 150 160 170 180 SGYASILGIP TLQTGVFGGI IIGALAAWCY NKFYNINLPS YLGFFAGKRF VPIMMATTSF 190 200 210 220 230 240 ILAFPMALIW PTIQSGLNAF STGLLDSNTG VAVFLFGFIK RLLIPFGLHH IFHAPFWFEF 250 260 270 280 290 300 GSWKNAAGEI IHGDQRIFIE QIREGAHLTA GKFMQGEFPV MMFGLPAAAL AIYHTAKPEN 310 320 330 340 350 360 KKVVAGLMGS AALTSFLTGI TEPLEFSFLF VAPLLFFIHA VLDGLSFLTL YLLDVHLGYT 370 380 390 400 410 420 FSGGFIDYVL LGVLPNKTQW WLVIPVGLVY AVIYYFVFRF LIVKLKYKTP GREDKQSQAV 430 440 450 460 470 480 TASATELPYA VLEAMGGKAN IKHLDACITR LRVEVNDKSK VDVPGLKDLG ASGVLEVGNN 490 500 510 520 530 540 MQAIFGPKSD QIKHEMQQIM NGQVVENPTT MEDDKDETVV VAEDKSATSE LSHIVHAPLT 550 560 570 580 590 600 GEVTPLSEVP DQVFSEKMMG DGIAIKPSQG EVRAPFNGKI QMIFPTKHAI GLVSDSGLEL 610 620 630 640 650 660 LIHIGLDTVK LNGEGFTLHV EEGQEVKQGD LLINFDLDYI RNHAKSDITP IIVTQGNITN 670 680 LDFKQGEHGN ISFGDQLFEA K

References

[1]

"Complete sequence of chromosome of Staphylococcus aureus subsp. aureus JH1." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Ivanova N., Tomasz A., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JH1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000736 Genomic DNA. Translation: ABR51041.1.
RefSeq	YP_001315328.1. NC_009632.1.
3D structure databases
ProteinModelPortal	A6TXX3.
SMR	A6TXX3. Positions 426-498.
ModBase	Search...
Protein-protein interaction databases
STRING	A6TXX3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000015802; EBSTAP00000015305; EBSTAG00000015801.
GeneID	5316627.
GenomeReviews	Gene locus SaurJH1_0179 in contig CP000736_GR.
KEGG	sah:SaurJH1_0179.
PATRIC	19532167. VBIStaAur98826_0214.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1263.
GeneTree	EBGT00050000023800.
HOGENOM	HBG571563.
OMA	CIPAIAL.
ProtClustDB	CLSK872840.
Enzyme and pathway databases
BioCyc	SAUR359787:SAURJH1_0179-MONOMER.
Family and domain databases
InterPro	IPR011055. Dup_hybrid_motif. IPR018113. PTrfase_EIIB/Cys_phosph_CS. IPR001127. PTS_EIIA_1_perm. IPR001996. PTS_EIIB_1. IPR003352. PTS_EIIC. IPR013013. PTS_EIIC_1. IPR011535. PTS_Glc-like_IIB_component. IPR011299. PTS_IIBC_glc. [Graphical view]
Gene3D	G3DSA:3.30.1360.60. PTS_EIIB. 1 hit.
KO	K02763. K02764. K02765.
Pfam	PF00358. PTS_EIIA_1. 1 hit. PF00367. PTS_EIIB. 1 hit. PF02378. PTS_EIIC. 1 hit. [Graphical view]
SUPFAM	SSF51261. Dup_hybrid_motif. 1 hit. SSF55604. PTS_EIIB. 1 hit.
TIGRFAMs	TIGR00826. EIIB_glc. 1 hit. TIGR00830. PTBA. 1 hit. TIGR02002. PTS-II-BC-glcB. 1 hit.
PROSITE	PS51093. PTS_EIIA_TYPE_1. 1 hit. PS00371. PTS_EIIA_TYPE_1_HIS. 1 hit. PS51098. PTS_EIIB_TYPE_1. 1 hit. PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit. PS51103. PTS_EIIC_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTG3C_STAA2

Accession

Primary (citable) accession number: A6TXX3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	August 21, 2007
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program