Reviewed, UniProtKB/Swiss-Prot A6TWW9 (AMPA_ALKMQ)

Names and origin

Protein names

Recommended name:
    Probable cytosol aminopeptidase
    EC=3.4.11.1
Alternative name(s):
    Leucine aminopeptidase
      Short name=LAP
    Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	Amet_4616

Organism

Alkaliphilus metalliredigens (strain QYMF) [Complete proteome] [HAMAP]

Taxonomic identifier

293826 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity.
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181
Cofactor	Binds 2 manganese ions per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP metalloexopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 500	500	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_1000058383
Sites
	Active site	280	1	Potential
	Active site	354	1	Potential
	Metal binding	268	1	Manganese 2 By similarity
	Metal binding	273	1	Manganese 1 By similarity
	Metal binding	273	1	Manganese 2 By similarity
	Metal binding	291	1	Manganese 2 By similarity
	Metal binding	350	1	Manganese 1 By similarity
	Metal binding	352	1	Manganese 1 By similarity
	Metal binding	352	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6TWW9-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: B8FF0ADAD4FDABA6	FASTA	500	53,606
	10 20 30 40 50 60 MKFKVINEKI SNLMVDILII GIYEEVKSLG DVHHTMDQQL GGLIQEMINA EEFKGEEGET 70 80 90 100 110 120 LLVHTLGRVP AKKCILLGLG KAEAFKENNL RNVVAKVMRE ARKSRAETIA ITPFGICNHI 130 140 150 160 170 180 SAEKVGQAIA EGTKLGLYQF NHYKTTAKEQ EERVLQEVYI LNEETGITAQ LQTGIDTGEK 190 200 210 220 230 240 LAHATMIARD LVNEPGNVLT PTEMAKRAQS ISQKHGLELE ILEKEDMERL GMGCFLGVTA 250 260 270 280 290 300 GSEEPPKLMV LKYNGGEEGG EILGLVGKGL TFDSGGISIK PGEGMDAMKG DMGGAAAVLG 310 320 330 340 350 360 AMEAIGALKP KTNVIAVVGA CENMPSGKAY KPGDILTSKG GKTVEILNTD AEGRLVLVDC 370 380 390 400 410 420 VSYALQLGAT RLVDLATLTG ACLIALGTTT TALISNDELW VKQIEDASKN AGEQVWQLPS 430 440 450 460 470 480 FPEYKEMIKS EIADLKNIGG KYAGAITAGL FVGEFAEGKP WVHMDIAGTS MSDKEKGYIT 490 500 KGGTGVAVRT LYELAKSMEK

References

[1]

"Complete sequence of Alkaliphilus metalliredigens QYMF." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Ye Q., Zhou J., Fields M., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000724 Genomic DNA. Translation: ABR50687.1.
RefSeq	YP_001322346.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6TWW9.
Protein family/group databases
MEROPS	M17.010.
Genome annotation databases
GeneID	5314779.
GenomeReviews	Gene locus Amet_4616 in contig CP000724_GR.
KEGG	amt:Amet_4616.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	CVVGLMH.
Family and domain databases
HAMAP	MF_00181. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR008283. Peptidase_M17_N. [Graphical view]
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_ALKMQ

Accession

Primary (citable) accession number: A6TWW9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	August 21, 2007
Last modified:	November 3, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)