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A6TWK7

- SYE_ALKMQ

UniProt

A6TWK7 - SYE_ALKMQ

Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Alkaliphilus metalliredigens (strain QYMF)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 54 (01 Oct 2014)
      Sequence version 1 (21 Aug 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

    Catalytic activityi

    ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi114 – 1141ZincUniRule annotation
    Metal bindingi116 – 1161ZincUniRule annotation
    Metal bindingi141 – 1411ZincUniRule annotation
    Metal bindingi143 – 1431ZincUniRule annotation
    Binding sitei261 – 2611ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW
    4. tRNA binding Source: InterPro

    GO - Biological processi

    1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciAMET293826:GI5P-4637-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
    Alternative name(s):
    Glutamyl-tRNA synthetaseUniRule annotation
    Short name:
    GluRSUniRule annotation
    Gene namesi
    Name:gltXUniRule annotation
    Ordered Locus Names:Amet_4503
    OrganismiAlkaliphilus metalliredigens (strain QYMF)
    Taxonomic identifieri293826 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeAlkaliphilus
    ProteomesiUP000001572: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 493493Glutamate--tRNA ligasePRO_0000330950Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi293826.Amet_4503.

    Structurei

    3D structure databases

    ProteinModelPortaliA6TWK7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi10 – 2011"HIGH" regionAdd
    BLAST
    Motifi258 – 2625"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0008.
    HOGENOMiHOG000252720.
    KOiK09698.
    OMAiKHYDGDF.
    OrthoDBiEOG6DRPF7.

    Family and domain databases

    Gene3Di1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPiMF_00022_B. Glu_tRNA_synth_B.
    InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR10119. PTHR10119. 1 hit.
    PfamiPF00749. tRNA-synt_1c. 1 hit.
    [Graphical view]
    PRINTSiPR00987. TRNASYNTHGLU.
    SUPFAMiSSF48163. SSF48163. 1 hit.
    TIGRFAMsiTIGR00464. gltX_bact. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A6TWK7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MESVRVRFAP SPTGFVHIGS LRTALYNYLF ARKNQGKYIL RVEDTDQSRF    50
    VEGAIEGMLK SMDWAGVSHN EGVVLEEETL KQQGQYGPYI QSERLPIYQE 100
    HITKLLEEGH AYHCFCSKER LDEVREKQKA AGETAKYDGH CRENPKEMVR 150
    EKIEAGESYV IRLKLPENRD IEFNDIVRGK VTMNTGDLDD QVLIKSDGFP 200
    TYHFAVVVDD HLMEITHVIR GEEWLPSTPK HVYLYEVMGW QAPQYVHLPN 250
    ILNTERKKLS KRQGDVAVGD FMRKGYLPEA LVNYIALVGW SPEDNQEIFS 300
    MTELEENFSL ERVSKSGGVF DVNKLNWINN HYIKESTADR IVDLAIPYLI 350
    EAGYVTEAEV GKKYDWLKDL VGVLKERLDY VADIVNHVDI FFKTAIEPKS 400
    DEAREILKEA HLPELLEAFL EKVEAAEVID DAFGKKALKE IQKEKGFKGP 450
    KLFKPIRVAL TGEEHGPDLP LIIKVLGKEN LKSRIQYVKE HLI 493
    Length:493
    Mass (Da):56,565
    Last modified:August 21, 2007 - v1
    Checksum:i057E0729AAE0BCB5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000724 Genomic DNA. Translation: ABR50575.1.
    RefSeqiWP_012065466.1. NC_009633.1.
    YP_001322234.1. NC_009633.1.

    Genome annotation databases

    EnsemblBacteriaiABR50575; ABR50575; Amet_4503.
    GeneIDi5314663.
    KEGGiamt:Amet_4503.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000724 Genomic DNA. Translation: ABR50575.1 .
    RefSeqi WP_012065466.1. NC_009633.1.
    YP_001322234.1. NC_009633.1.

    3D structure databases

    ProteinModelPortali A6TWK7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 293826.Amet_4503.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABR50575 ; ABR50575 ; Amet_4503 .
    GeneIDi 5314663.
    KEGGi amt:Amet_4503.

    Phylogenomic databases

    eggNOGi COG0008.
    HOGENOMi HOG000252720.
    KOi K09698.
    OMAi KHYDGDF.
    OrthoDBi EOG6DRPF7.

    Enzyme and pathway databases

    BioCyci AMET293826:GI5P-4637-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPi MF_00022_B. Glu_tRNA_synth_B.
    InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR10119. PTHR10119. 1 hit.
    Pfami PF00749. tRNA-synt_1c. 1 hit.
    [Graphical view ]
    PRINTSi PR00987. TRNASYNTHGLU.
    SUPFAMi SSF48163. SSF48163. 1 hit.
    TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of Alkaliphilus metalliredigens QYMF."
      Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M.
      , Hauser L., Kyrpides N., Mikhailova N., Ye Q., Zhou J., Fields M., Richardson P.
      Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: QYMF.

    Entry informationi

    Entry nameiSYE_ALKMQ
    AccessioniPrimary (citable) accession number: A6TWK7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: August 21, 2007
    Last modified: October 1, 2014
    This is version 54 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3