Reviewed, UniProtKB/Swiss-Prot A6TWK5 (SYC_ALKMQ)

Names and origin

Protein names

Recommended name:
    Cysteinyl-tRNA synthetase
    EC=6.1.1.16
Alternative name(s):
    Cysteine--tRNA ligase
      Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	Amet_4501

Organism

Alkaliphilus metalliredigens (strain QYMF) [Complete proteome] [HAMAP]

Taxonomic identifier

293826 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00041
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cysteine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	Cysteinyl-tRNA synthetase HAMAP MF_00041		PRO_0000332784
Regions
	Motif	29 – 39	11	"HIGH" region HAMAP MF_00041
	Motif	264 – 268	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	27	1	Zinc By similarity
	Metal binding	207	1	Zinc By similarity
	Metal binding	232	1	Zinc By similarity
	Metal binding	236	1	Zinc By similarity
	Binding site	267	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6TWK5-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 71264342F7859A54	FASTA	464	53,550
	10 20 30 40 50 60 MRLYNTLTRK KEVFVPIEQG KVRMYACGPT VYNYFHIGNG RTFIVFDALR KYLVYRGYDV 70 80 90 100 110 120 TFVQNFTDID DKIIKKAQEE AVSAKEISER FIVEYFKDAA TLGVDQADIH PKVTENVPEI 130 140 150 160 170 180 IAFIQELLEK NVAYEVNGDV YYDVSRFEDY GKLSKQNLEE LQSGARIEVN EAKRNSLDFV 190 200 210 220 230 240 LWKTAKPGEP SWESPWGSGR PGWHIECSAM AKKYLGETID IHGGGGDLVF PHHENEIAQS 250 260 270 280 290 300 EACTGKTFAN YWIHVGYLNI DNKKMSKSLD NFFTPREIAE EYDLESLRFF MLSAHYRTPV 310 320 330 340 350 360 NFSRELLESA KSGLERLYNA KNHLEHILEN TKVEEMKETE KEWIDSLTHY SEEFNRVMDD 370 380 390 400 410 420 DFNTADGIAV IFDLVRDMNT YITGEASKEA VKAAYGMLVE LTGVLGLLRK EEESLDKEIG 430 440 450 460 RLIEARQQAR IEKNFTKSDE IRDYLKEKGI VLEDTSQGVK WRKE

References

[1]

"Complete sequence of Alkaliphilus metalliredigens QYMF." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Ye Q., Zhou J., Fields M., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000724 Genomic DNA. Translation: ABR50573.1.
RefSeq	YP_001322232.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6TWK5.
Genome annotation databases
GeneID	5314661.
GenomeReviews	Gene locus Amet_4501 in contig CP000724_GR.
KEGG	amt:Amet_4501.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VLWKAAK.
Family and domain databases
HAMAP	MF_00041. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR015804. Cys-tRNA-synt_Ia_C. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR015803. Cys-tRNA-synt_Ia_N. IPR002308. Cys-tRNA-synth_1a. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
TIGRFAMs	TIGR00435. cysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_ALKMQ

Accession

Primary (citable) accession number: A6TWK5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	August 21, 2007
Last modified:	November 3, 2009
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)