A6TWI5 (EFG_ALKMQ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor G
Short name=EF-G

Gene names

Name:	fusA
Ordered Locus Names:	Amet_4481

Organism

Alkaliphilus metalliredigens (strain QYMF) [Complete proteome] [HAMAP]

Taxonomic identifier

293826 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus

Protein attributes

Sequence length	689 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome By similarity. HAMAP MF_00054_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00054_B.
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Elongation factor
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 689	689	Elongation factor G HAMAP MF_00054_B		PRO_1000057384
Regions
	Nucleotide binding	17 – 24	8	GTP By similarity
	Nucleotide binding	81 – 85	5	GTP By similarity
	Nucleotide binding	135 – 138	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6TWI5 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 7527CDA2C134FD65	FASTA	689	76,079
	10 20 30 40 50 60 MPREFPLERT RNIGIMAHID AGKTTTTERI LFYSGTTRKL GETHEGASQM DWMDQEKERG 70 80 90 100 110 120 ITITSAATTC QWLNHRVNII DTPGHVDFTV EVERSLRVLD GAVAVFCAKG GVEPQSETVW 130 140 150 160 170 180 RQGDKYKVPR MAFVNKMDRQ GADFFNVMQM MIDRLAANPV AVQLPIGSEE EFTGIIDLVK 190 200 210 220 230 240 MEATIYLDDV GQETKIVEIP EELKELAVEY REKLVEAVSE TSEELMMKYL EGEALTEEEL 250 260 270 280 290 300 VKGIRQGTIN VQITPVYCGS AYKNKGVQLL LDGVVAYMPS PLDIPAISGI DADTQEEIER 310 320 330 340 350 360 HADDSEPFSA LAFKIMADPY VGKLAFFRVY SGTLDAGSYV LNSTKGKKER IGRILQMHAN 370 380 390 400 410 420 TRAEIPTVYA GDIAAAVGLK DTTTGDTLCD PTDAVILESM VFPEPVISVA IEPKTKAGQE 430 440 450 460 470 480 KMGVALQKLA EEDPTFKTYT DEETSQTIIA GMGELHLEII VDRMMREFKV EATVGKPQVA 490 500 510 520 530 540 YKETITKAVE VEAKYAKQSG GRGQYGHVKI RMIPQEPGIG YEFTNSTVGG SIPREYVPAV 550 560 570 580 590 600 DQGIQGAMVN GILAGYEVVD FKVELYDGSY HDVDSSEMAF KIAGSMAFKE GMRKASPALL 610 620 630 640 650 660 EPYMKVEVVT PEDYMGDVIG DLNSRRGQIE GMESRSGAQV IKAFVPLSEM FGYSTDLRSK 670 680 TQGRATYSMH FNHYAQVPAS ISEKIMTGK

References

[1]

"Complete sequence of Alkaliphilus metalliredigens QYMF." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Ye Q., Zhou J., Fields M., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: QYMF.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000724 Genomic DNA. Translation: ABR50553.1.
RefSeq	YP_001322212.1. NC_009633.1.
3D structure databases
ProteinModelPortal	A6TWI5.
SMR	A6TWI5. Positions 4-686.
ModBase	Search...
Protein-protein interaction databases
STRING	A6TWI5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5314641.
GenomeReviews	Gene locus Amet_4481 in contig CP000724_GR.
KEGG	amt:Amet_4481.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0480.
HOGENOM	HBG737692.
OMA	CNEWREK.
ProtClustDB	PRK00007.
Enzyme and pathway databases
BioCyc	AMET293826:AMET_4481-MONOMER.
Family and domain databases
HAMAP	MF_00054_B. EF-G_EF-2_B. [Tree]
InterPro	IPR009022. Elongation_fac_G/III/V. IPR000795. ProtSyn_GTP-bd. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR005225. Small_GTP-bd_dom. IPR004540. Transl_elong_EFG/EF2. IPR000640. Transl_elong_EFG/EF2_C. IPR005517. Transl_elong_EFG/EF2_IV. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit. G3DSA:3.30.70.240. Transl_elong_EFG/EF2_C. 1 hit.
KO	K02355.
Pfam	PF00679. EFG_C. 1 hit. PF03764. EFG_IV. 1 hit. PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SMART	SM00838. EFG_C. 1 hit. SM00889. EFG_IV. 1 hit. [Graphical view]
SUPFAM	SSF54980. EFG_III_V. 2 hits. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00484. EF-G. 1 hit. TIGR00231. Small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EFG_ALKMQ

Accession

Primary (citable) accession number: A6TWI5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	August 21, 2007
Last modified:	November 16, 2011
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program