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A6TWC9

- LIPA_ALKMQ

UniProt

A6TWC9 - LIPA_ALKMQ

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Protein
Lipoyl synthase
Gene
lipA, Amet_4425
Organism
Alkaliphilus metalliredigens (strain QYMF)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi36 – 361Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi41 – 411Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi47 – 471Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi62 – 621Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi66 – 661Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi69 – 691Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciAMET293826:GI5P-4559-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:Amet_4425
OrganismiAlkaliphilus metalliredigens (strain QYMF)
Taxonomic identifieri293826 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeAlkaliphilus
ProteomesiUP000001572: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 290290Lipoyl synthaseUniRule annotation
PRO_1000058572Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi293826.Amet_4425.

Structurei

3D structure databases

ProteinModelPortaliA6TWC9.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235998.
KOiK03644.
OMAiHTICQEA.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

A6TWC9-1 [UniParc]FASTAAdd to Basket

« Hide

MNIKRKPEWL RIKLGEGRNL NYVKGLLKKF SLNTVCEEAN CPNQIECFSK    50
KTATFMILGS DCSRSCGFCN VSHGALQPID PNEPENVANA VAELGLKHVV 100
ITSVTRDDLA DGGAQHFADV VNQIKSKNKE TMIEVLIPDF QGNKEALQKV 150
VQSKPDIINH NMETIPRLYP EIRPKAEYVQ SLELLKNVKE MDPEILTKSG 200
VMVGLGEGEE ELIEVFKDLR GSGCDFLTVG QYLPPSTKHY PLKAYISPEV 250
FERYKEEALK IGFSFVASSP LVRSSYNAAE ALEKHEEMKS 290
Length:290
Mass (Da):32,420
Last modified:August 21, 2007 - v1
Checksum:iBF23B910109A2A01
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000724 Genomic DNA. Translation: ABR50497.1.
RefSeqiYP_001322156.1. NC_009633.1.

Genome annotation databases

EnsemblBacteriaiABR50497; ABR50497; Amet_4425.
GeneIDi5314585.
KEGGiamt:Amet_4425.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000724 Genomic DNA. Translation: ABR50497.1 .
RefSeqi YP_001322156.1. NC_009633.1.

3D structure databases

ProteinModelPortali A6TWC9.
ModBasei Search...

Protein-protein interaction databases

STRINGi 293826.Amet_4425.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABR50497 ; ABR50497 ; Amet_4425 .
GeneIDi 5314585.
KEGGi amt:Amet_4425.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235998.
KOi K03644.
OMAi HTICQEA.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci AMET293826:GI5P-4559-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of Alkaliphilus metalliredigens QYMF."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M.
    , Hauser L., Kyrpides N., Mikhailova N., Ye Q., Zhou J., Fields M., Richardson P.
    Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: QYMF.

Entry informationi

Entry nameiLIPA_ALKMQ
AccessioniPrimary (citable) accession number: A6TWC9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: August 21, 2007
Last modified: May 14, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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