Reviewed, UniProtKB/Swiss-Prot A6TVS9 (SYN_ALKMQ)

Names and origin

Protein names

Recommended name:
    Asparaginyl-tRNA synthetase
    EC=6.1.1.22
Alternative name(s):
    Asparagine--tRNA ligase
      Short name=AsnRS

Gene names

Name:	asnS
Ordered Locus Names:	Amet_4217

Organism

Alkaliphilus metalliredigens (strain QYMF) [Complete proteome] [HAMAP]

Taxonomic identifier

293826 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00534
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP asparagine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 463	463	Asparaginyl-tRNA synthetase HAMAP MF_00534		PRO_1000061016

Sequences

	Sequence		Length	Mass (Da)
	A6TVS9-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 25941D2D2606CA5F	FASTA	463	53,532
	10 20 30 40 50 60 MKTVSVKEIY RQTEQYAGQS IRVEGWIRTL RASKAFGFIE LNDGTFFKNI QIVFDEDLHN 70 80 90 100 110 120 FSDVSKFTIS SAIIIEGTLE ITPGAKQPFE IKATNVELEA HSHKDYPLQK KRHTFEYLRQ 130 140 150 160 170 180 IAHLRPRSNA FSAVFRVRSI AAFAIHQFFQ EKGFVYTHTP IVTGSDAEGA GEMFRVSTLD 190 200 210 220 230 240 FKNIPLDEEQ QVDFKKDFFG KETSLTVSGQ LAAEAYALAF KKVYTFGPTF RAENSNTARH 250 260 270 280 290 300 ASEFWMIEPE IAFADLQDNM ELAEDMLKYM IRYVMEQAPE ELEFFNQFVD KGLIDRLNHV 310 320 330 340 350 360 ANSEFGKVTY TEAVKMLQES GEEFEYPVEW GHDLQTEHER YLTEKVFKKP VFVMDYPKDI 370 380 390 400 410 420 KAFYMRMNDD NKTVAAMDLL VPGIGEIIGG SQREERLDVL TDRMKQMGLD EEEYWWYLEL 430 440 450 460 RKYGGVKHAG YGLGFERAIM YLTGMANIRD VISYPRTPKS AEF

References

[1]

"Complete sequence of Alkaliphilus metalliredigens QYMF." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Ye Q., Zhou J., Fields M., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000724 Genomic DNA. Translation: ABR50297.1.
RefSeq	YP_001321956.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6TVS9.
Genome annotation databases
GeneID	5314298.
GenomeReviews	Gene locus Amet_4217 in contig CP000724_GR.
KEGG	amt:Amet_4217.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	LQKKRHS.
Family and domain databases
HAMAP	MF_00534. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp-tRNA-synth_IIb. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00457. asnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN_ALKMQ

Accession

Primary (citable) accession number: A6TVS9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	August 21, 2007
Last modified:	November 3, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)