Reviewed, UniProtKB/Swiss-Prot A6TUA0 (PROA_ALKMQ)

Names and origin

Protein names

Recommended name:
    Gamma-glutamyl phosphate reductase
      Short name=GPR
    EC=1.2.1.41
Alternative name(s):
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
      Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	Amet_3646

Organism

Alkaliphilus metalliredigens (strain QYMF) [Complete proteome] [HAMAP]

Taxonomic identifier

293826 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus

Protein attributes

Sequence length	414 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate By similarity.
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP(+) = L-glutamyl 5-phosphate + NADPH.
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 414	414	Gamma-glutamyl phosphate reductase		PRO_1000080475

Sequences

	Sequence		Length	Mass (Da)
	A6TUA0-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: D380FB3534B97064	FASTA	414	45,563
	10 20 30 40 50 60 MMTNLMEQCK VLQNASKQLS SMNTKDKNEA LRQVAISLQK NESFIIQENQ KDVENAEAKG 70 80 90 100 110 120 MKESLVDRLR LTKDRVEGMI KGIQTIIELK DPVWKSNEVW TLENGLTVSK MTVPIGVIGI 130 140 150 160 170 180 IYESRPNVTV DAFCLTLKSG NGVLLRGSST SIFSNRALVH AIKEGLKNSD VSEAVISLID 190 200 210 220 230 240 DPDRGLVKEM LTLNEYIDLI IPRGGKELID FVVKNATVPT IETGVGNCHI FVDESANIDE 250 260 270 280 290 300 AVDIIENAKV QRPGVCNACE TVLIHEKIAK DLLPKVSGRI GKQVEIRGCQ HTQSLIGGKL 310 320 330 340 350 360 AIEDDWAEEF LDYIVAVKVV PNVDEAIEHI NHFGTKHSEA ILTENLSHAN QFLRQVDAAA 370 380 390 400 410 VYVNASTRFT DGSEFGFGGE MGISTQKIHA RGPMGLNELV TVKYTIVGNG QIRK

References

[1]

"Complete sequence of Alkaliphilus metalliredigens QYMF." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Ye Q., Zhou J., Fields M., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000724 Genomic DNA. Translation: ABR49768.1.
RefSeq	YP_001321427.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5313727.
GenomeReviews	Gene locus Amet_3646 in contig CP000724_GR.
KEGG	amt:Amet_3646.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00412. [Tree]
InterPro	IPR016163. Ald_DHase_C. IPR016162. Ald_DHase_N. IPR000965. Gglut_pp_reduct. IPR012134. Glu-5-SA_DHase. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 2 hits.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
PIRSF	PIRSF000151. GPR. 1 hit.
TIGRFAMs	TIGR00407. proA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_ALKMQ

Accession

Primary (citable) accession number: A6TUA0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	August 21, 2007
Last modified:	November 25, 2008
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)