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Protein

Glutamate 5-kinase

Gene

proB

Organism
Alkaliphilus metalliredigens (strain QYMF)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB), Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei15ATPUniRule annotation1
Binding sitei55SubstrateUniRule annotation1
Binding sitei142SubstrateUniRule annotation1
Binding sitei154Substrate; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi174 – 175ATPUniRule annotation2
Nucleotide bindingi216 – 222ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAmino-acid biosynthesis, Proline biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAMET293826:G1G94-3646-MONOMER
UniPathwayiUPA00098; UER00359

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:Amet_3645
OrganismiAlkaliphilus metalliredigens (strain QYMF)
Taxonomic identifieri293826 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeAlkaliphilus
Proteomesi
  • UP000001572 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001936841 – 264Glutamate 5-kinaseAdd BLAST264

Proteomic databases

PRIDEiA6TU99

Interactioni

Protein-protein interaction databases

STRINGi293826.Amet_3645

Structurei

3D structure databases

ProteinModelPortaliA6TU99
SMRiA6TU99
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CGT Bacteria
COG0263 LUCA
HOGENOMiHOG000246368
KOiK00931
OMAiPLHTRFI
OrthoDBiPOG091H00OF

Family and domain databases

Gene3Di3.40.1160.10, 1 hit
HAMAPiMF_00456 ProB, 1 hit
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR001048 Asp/Glu/Uridylate_kinase
IPR001057 Glu/AcGlu_kinase
IPR011529 Glu_5kinase
IPR005715 Glu_5kinase/COase_Synthase
IPR019797 Glutamate_5-kinase_CS
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PIRSFiPIRSF000729 GK, 1 hit
PRINTSiPR00474 GLU5KINASE
SUPFAMiSSF53633 SSF53633, 1 hit
TIGRFAMsiTIGR01027 proB, 1 hit
PROSITEiView protein in PROSITE
PS00902 GLUTAMATE_5_KINASE, 1 hit

Sequencei

Sequence statusi: Complete.

A6TU99-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIEELINKSK KIVIKIGSNT LSNDNGTINK NFLKELSEQI IYLRDKGKQF
60 70 80 90 100
VIVSSGARIA GVSTLGKWMR KEDMNYKQAL CAIGQVELMD AYRRCFEPYN
110 120 130 140 150
VFIAQMLLTR DDFSDAHRRL NIRNTLFTLV DEGVIPIINE NDTVSVEEIR
160 170 180 190 200
IGDNDTLAAL TTNIWNADLL ILFSDIDGIY DKNPKEHEDA ILVENVMNID
210 220 230 240 250
ELLEKIEVGD VNEFGTGGIA TKIEAAKAVN DYGIPMILAN GKKENILLKL
260
LEGTEKATIF QAGK
Length:264
Mass (Da):29,571
Last modified:August 21, 2007 - v1
Checksum:iC71C4747EAC9C908
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000724 Genomic DNA Translation: ABR49767.1
RefSeqiWP_012064727.1, NC_009633.1

Genome annotation databases

EnsemblBacteriaiABR49767; ABR49767; Amet_3645
KEGGiamt:Amet_3645

Similar proteinsi

Entry informationi

Entry nameiPROB_ALKMQ
AccessioniPrimary (citable) accession number: A6TU99
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: August 21, 2007
Last modified: May 23, 2018
This is version 65 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health