A6TU32 (TPIS_ALKMQ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Triosephosphate isomerase
Short name=TIM
EC=5.3.1.1

Alternative name(s):
Triose-phosphate isomerase

Gene names

Name:	tpiA
Ordered Locus Names:	Amet_3578

Organism

Alkaliphilus metalliredigens (strain QYMF) [Complete proteome] [HAMAP]

Taxonomic identifier

293826 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate = glycerone phosphate. HAMAP MF_00147_B
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00147_B Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1. HAMAP MF_00147_B
Subunit structure	Homodimer By similarity. HAMAP MF_00147_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00147_B.
Sequence similarities	Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
Biological process	Gluconeogenesis Glycolysis Pentose shunt
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-KW glycolysis Inferred from electronic annotation. Source: UniProtKB-KW pentose-phosphate shunt Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triose-phosphate isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 248	248	Triosephosphate isomerase HAMAP MF_00147_B		PRO_1000058106
Sites
	Active site	94	1	Electrophile By similarity
	Active site	166	1	Proton acceptor By similarity
	Binding site	9	1	Substrate By similarity
	Binding site	11	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6TU32 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 5E0F6227EE3ACE26	FASTA	248	27,484
	10 20 30 40 50 60 MRKPIIAGNW KMNKTVAETL QFINEIRDQA ETTDVEVVLC CPFTALSEAK KALGASKIKL 70 80 90 100 110 120 GAQNMHWEDN GAFTGEISAE MLKELGVDYI LIGHSERRQY FNETDETVNK KIKKALEQKL 130 140 150 160 170 180 MPILCVGETL QEREAAQTFD VIKNQITIDL EGILPDSMKD IVIAYEPIWA IGTGKTASSQ 190 200 210 220 230 240 DANHVIAYIR QLIQEKYDDG ISETVRIQYG GSVKPENATE IMNEEDIDGA LVGGASLKAD AFLGIVNF

References

[1]

"Complete sequence of Alkaliphilus metalliredigens QYMF." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Ye Q., Zhou J., Fields M., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: QYMF.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000724 Genomic DNA. Translation: ABR49700.1.
RefSeq	YP_001321359.1. NC_009633.1.
3D structure databases
ProteinModelPortal	A6TU32.
SMR	A6TU32. Positions 2-248.
ModBase	Search...
Protein-protein interaction databases
STRING	A6TU32.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5313659.
GenomeReviews	Gene locus Amet_3578 in contig CP000724_GR.
KEGG	amt:Amet_3578.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0149.
HOGENOM	HBG708281.
OMA	VCVGETQ.
ProtClustDB	PRK00042.
Enzyme and pathway databases
BioCyc	AMET293826:AMET_3578-MONOMER.
Family and domain databases
HAMAP	MF_00147_B. TIM_B. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR022896. TrioseP_Isoase_bac/euk. IPR000652. Triosephosphate_isomerase. IPR020861. Triosephosphate_isomerase_AS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01803.
PANTHER	PTHR21139. Triophos_ismrse. 1 hit.
Pfam	PF00121. TIM. 1 hit. [Graphical view]
SUPFAM	SSF51351. Triophos_ismrse. 1 hit.
TIGRFAMs	TIGR00419. Tim. 1 hit.
PROSITE	PS00171. TIM_1. 1 hit. PS51440. TIM_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPIS_ALKMQ

Accession

Primary (citable) accession number: A6TU32

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	August 21, 2007
Last modified:	November 16, 2011
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program