Reviewed, UniProtKB/Swiss-Prot A6TTJ1 (ARGB_ALKMQ)

Names and origin

Protein names

Recommended name:
    Acetylglutamate kinase
    EC=2.7.2.8
Alternative name(s):
    NAG kinase
      Short name=AGK
    N-acetyl-L-glutamate 5-phosphotransferase

Gene names

Name:	argB
Ordered Locus Names:	Amet_3381

Organism

Alkaliphilus metalliredigens (strain QYMF) [Complete proteome] [HAMAP]

Taxonomic identifier

293826 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate. HAMAP MF_00082
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4. HAMAP MF_00082
Subcellular location	Cytoplasm Probable HAMAP MF_00082.
Sequence similarities	Belongs to the acetylglutamate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP acetylglutamate kinase activity Inferred from electronic annotation. Source: HAMAP glutamate 5-kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	Acetylglutamate kinase HAMAP MF_00082		PRO_1000057536
Regions
	Region	65 – 66	2	Substrate binding By similarity
Sites
	Binding site	87	1	Substrate By similarity
	Binding site	179	1	Substrate By similarity
	Site	30	1	Transition state stabilizer By similarity
	Site	242	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6TTJ1-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 273F726C4371E2CC	FASTA	316	34,334
	10 20 30 40 50 60 MNLSPNQKAD VLIESLPYIK KFYNEIVVIK YGGSAMVNDE LKESIIKDIV LMKYVGMHPV 70 80 90 100 110 120 VVHGGGPEIT NMLKVFDKES KFVDGLRVTD QATMEITEMV LLGKVCQEIV TRINQNGVRS 130 140 150 160 170 180 IGICGKDGSM IKTVPKDPEL GLVGGITSID TDLIKTIIEK GYIPVISPIG CGPAGESHNI 190 200 210 220 230 240 NADEVAGKLA AALGAKKLML ITDVAGVLRD QHDEASLISD IKTNEIEEYI ETGVIKGGMI 250 260 270 280 290 300 PKISCCFDAV ANGVERAHII DGRKSHSMLL EIFTDQGVGT MITHGGDQNG NSKNNKQWKS 310 IFNEHLWENA YSLPGR

References

[1]

"Complete sequence of Alkaliphilus metalliredigens QYMF." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Ye Q., Zhou J., Fields M., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000724 Genomic DNA. Translation: ABR49509.1.
RefSeq	YP_001321168.1.
3D structure databases
SMR	A6TTJ1. Positions 8-283.
ModBase	Search...
Protein-protein interaction databases
STRING	A6TTJ1.
Genome annotation databases
GeneID	5313461.
GenomeReviews	Gene locus Amet_3381 in contig CP000724_GR.
KEGG	amt:Amet_3381.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0548.
HOGENOM	HBG497643.
OMA	MDIVEMV.
Family and domain databases
HAMAP	MF_00082_B. ArgB_B. [Tree]
InterPro	IPR004662. AcgluKinase. IPR001048. Asp/Glu/Uridylate_kinase. IPR011148. GlcNAc_kinase. IPR001057. Glu_5kinase. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000728. NAGK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
TIGRFAMs	TIGR00761. argB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGB_ALKMQ

Accession

Primary (citable) accession number: A6TTJ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	August 21, 2007
Last modified:	February 9, 2010
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)