Reviewed, UniProtKB/Swiss-Prot A6TS64 (MRAY_ALKMQ)

Names and origin

Protein names

Recommended name:
    Phospho-N-acetylmuramoyl-pentapeptide-transferase
    EC=2.7.8.13
Alternative name(s):
    UDP-MurNAc-pentapeptide phosphotransferase

Gene names

Name:	mraY
Ordered Locus Names:	Amet_2882

Organism

Alkaliphilus metalliredigens (strain QYMF) [Complete proteome] [HAMAP]

Taxonomic identifier

293826 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan By similarity.
Catalytic activity	UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate = UMP + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol. HAMAP MF_00038
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00038
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 4 family. MraY subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	phospho-N-acetylmuramoyl-pentapeptide-transferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 331	331	Phospho-N-acetylmuramoyl-pentapeptide-transferase HAMAP MF_00038		PRO_0000332524
Regions
	Transmembrane	7 – 27	21	Potential
	Transmembrane	54 – 74	21	Potential
	Transmembrane	78 – 98	21	Potential
	Transmembrane	106 – 126	21	Potential
	Transmembrane	133 – 153	21	Potential
	Transmembrane	154 – 174	21	Potential
	Transmembrane	195 – 215	21	Potential
	Transmembrane	249 – 269	21	Potential
	Transmembrane	311 – 331	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	A6TS64-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: D31B31AFD4F5FFA9	FASTA	331	35,448
	10 20 30 40 50 60 MMNHNQIIYT IIIGFIITLI LGPLTIPFLR RLKVGQTIRE EGPKTHMAKS GTPTIGGIIL 70 80 90 100 110 120 IMSIIITSLT SGLINEELWI ALAATVAFGI IGFIDDFIKV ILKRNLGLRA YQKLILQGTI 130 140 150 160 170 180 AVILAIYQSR TSIMGTEVIV PFVGEGITIA GFTITQTIDL GILYIPFIVF VVVATVNSVN 190 200 210 220 230 240 LTDGLDGLAA GVTLIIAAFF ALVAMSWGYV SLAIFAAAIT GACLGFLKFN SHPAQVFMGD 250 260 270 280 290 300 TGSLALGGAI ATIAVLMNVV LIIPIVGGIY FAEAVSVILQ VISFKLTGKR IFKMSPLHHH 310 320 330 YELSGWAETK VVIVFWVVTV ILCLVGMLAL S

References

[1]

"Complete sequence of Alkaliphilus metalliredigens QYMF." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Ye Q., Zhou J., Fields M., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000724 Genomic DNA. Translation: ABR49032.1.
RefSeq	YP_001320691.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6TS64.
Genome annotation databases
GeneID	5312956.
GenomeReviews	Gene locus Amet_2882 in contig CP000724_GR.
KEGG	amt:Amet_2882.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	HHHFEQK.
Family and domain databases
HAMAP	MF_00038. [Tree]
InterPro	IPR000715. Glycosyl_transferase_4. IPR018481. Glycosyl_Trfase_4_cons-reg. IPR003524. PNAcMuramoyl-5peptid_Trfase. IPR018480. PNAcMuramoyl-5peptid_Trfase_CS. [Graphical view]
PANTHER	PTHR22926. Glyco_trans_4. 1 hit. PTHR22926:SF3. PNAcPpept_trans. 1 hit.
Pfam	PF00953. Glycos_transf_4. 1 hit. PF10555. MraY_sig1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00445. mraY. 1 hit.
PROSITE	PS01347. MRAY_1. False negative. PS01348. MRAY_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MRAY_ALKMQ

Accession

Primary (citable) accession number: A6TS64

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	August 21, 2007
Last modified:	November 3, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)