A6TRS6 (TRMD_ALKMQ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(1)-)-methyltransferase
EC=2.1.1.228

Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	Amet_2743

Organism

Alkaliphilus metalliredigens (strain QYMF) [Complete proteome] [HAMAP]

Taxonomic identifier

293826 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus

Protein attributes

Sequence length	247 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605
Catalytic activity	S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. HAMAP MF_00605
Subunit structure	Homodimer By similarity. HAMAP MF_00605
Subcellular location	Cytoplasm Potential HAMAP MF_00605.
Sequence similarities	Belongs to the RNA methyltransferase TrmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 247	247	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_1000061266
Regions
	Region	135 – 140	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	115	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6TRS6 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 52F299A929752BB5	FASTA	247	28,173
	10 20 30 40 50 60 MKVRILTLFP EMFNGPFGTS ILKKAQEKEL IDIQCFNIRD FALNKHKKVD DYPFGGGAGM 70 80 90 100 110 120 VMTPQPIFDC HHHVTQMLNL NEPCKTIYLS PKGSTFTQEK AMELAKEEQL IFLCGHYEGI 130 140 150 160 170 180 DQRIIDELVT DEISIGDYVL TGGELPAMVI VDAITRLIPG VLSTEASYED ESFHCGLLEY 190 200 210 220 230 240 PHYTRPRAFN GLEVPSVLLS GNHKDIDMWR RKQSIELTFE RRPDLLKGLQ LKKNEAQWVA ELSKKKE

References

[1]

"Complete sequence of Alkaliphilus metalliredigens QYMF." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Ye Q., Zhou J., Fields M., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: QYMF.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000724 Genomic DNA. Translation: ABR48894.1.
RefSeq	YP_001320553.1. NC_009633.1.
3D structure databases
ProteinModelPortal	A6TRS6.
ModBase	Search...
Protein-protein interaction databases
STRING	A6TRS6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5312815.
GenomeReviews	Gene locus Amet_2743 in contig CP000724_GR.
KEGG	amt:Amet_2743.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0336.
HOGENOM	HBG285805.
OMA	VCGRFEG.
ProtClustDB	PRK00026.
Enzyme and pathway databases
BioCyc	AMET293826:AMET_2743-MONOMER.
Family and domain databases
HAMAP	MF_00605. TrmD. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. IPR023148. tRNA_m1G_MeTrfase_C. [Graphical view]
Gene3D	G3DSA:1.10.1270.20. tRNA_m1G_MeTrfase_C. 1 hit.
KO	K00554.
PANTHER	PTHR10056. PTHR10056. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMs	TIGR00088. TrmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_ALKMQ

Accession

Primary (citable) accession number: A6TRS6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	August 21, 2007
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program