A6TQZ4 (SYA_ALKMQ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Amet_2458

Organism

Alkaliphilus metalliredigens (strain QYMF) [Complete proteome] [HAMAP]

Taxonomic identifier

293826 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus

Protein attributes

Sequence length	880 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036_B
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036_B
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00036_B.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036_B
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.
Sequence caution	The sequence ABR48612.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 880	880	Alanine--tRNA ligase HAMAP MF_00036_B		PRO_0000347485
Sites
	Metal binding	566	1	Zinc Potential
	Metal binding	570	1	Zinc Potential
	Metal binding	668	1	Zinc Potential
	Metal binding	672	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	A6TQZ4 [UniParc]. Last modified September 2, 2008. Version 2. Checksum: 6C28E39B758456C9	FASTA	880	98,968
	10 20 30 40 50 60 MKKMGLNEIR KLFLDFYEEK EHYVQSSYPL VPHNDKSLLL INAGMAPLKN YFSGVETPPS 70 80 90 100 110 120 KRMATCQKCI RTGDIENVGK TSRHATFFEM LGSFAFGDYF KTESIQWGWE FATKYLEMPE 130 140 150 160 170 180 DKIWASVYEE DDEAYGIWEN QIKMPKERIV RLGKEDNFWE IGVGPCGPCS ELYFDRGDKY 190 200 210 220 230 240 SCGHDDCKPG CDCDRFVEFW NHVFTQFDKD EAGNYNLLPN PNIDTGMGLE RVACIMQDVD 250 260 270 280 290 300 SIFEVDTMKH ILNSVCTATN TQYNKDVKTN ISLRIITDHL RSITFMIGDG ILPSNEGRGY 310 320 330 340 350 360 VLRRLLRRAA RHGKLLGVSK SFLYELMDTV TETYGGAYSE LVEKKDYIKK IIQVEEDRFQ 370 380 390 400 410 420 ETIHQGLEIL NQHIEEMIGK SENMLNGTYA FKLYDTYGFP LDLTKEILEE REMTVDESEF 430 440 450 460 470 480 ESEMEKQRNR ARKARSGGDT EGWKEDAFDA LDKNIQTSFK GYETLRAEGK VLAIIEENQS 490 500 510 520 530 540 VNLTSAGKEV VIVLDKTPFY PESGGQIGDI GHIFKDEFEG QVLDTKQGKN QRIHQYIKIL 550 560 570 580 590 600 RGILQVGDSI QGEVDKEPRH NTERNHTATH LLHKALKGII GEHVEQAGSL VTPEKLRFDF 610 620 630 640 650 660 SHFEGLSSAD LSKVELEVNQ EILNALNVDT VEASLEEAKK MGAMALFGEK YGDDVRVVKT 670 680 690 700 710 720 GDYSVELCGG THVKNSSEIG TFLILSETGV AAGVRRIEAV TGQEAYHHIK REQGLIQDIE 730 740 750 760 770 780 SLLKTKGEQL TKRVEELLKE TKEKDKELQQ LKSKLANQSI DEILNQIEVI EGTNLLVHHF 790 800 810 820 830 840 GEQSMEDLRN IGDSLKQKIG SGVIALGAES NDKASFFVTA TKDVVEKGVH SGNMIREVAK 850 860 870 880 IAGGGGGGRP DMAQAGGKNP EKIQSALSIV KGLLKNQLNG

References

[1]

"Complete sequence of Alkaliphilus metalliredigens QYMF." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Ye Q., Zhou J., Fields M., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: QYMF.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000724 Genomic DNA. Translation: ABR48612.1. Different initiation.
RefSeq	YP_001320271.1. NC_009633.1.
3D structure databases
ProteinModelPortal	A6TQZ4.
ModBase	Search...
Protein-protein interaction databases
STRING	A6TQZ4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5312530.
GenomeReviews	Gene locus Amet_2458 in contig CP000724_GR.
KEGG	amt:Amet_2458.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0013.
HOGENOM	HBG354397.
ProtClustDB	PRK00252.
Enzyme and pathway databases
BioCyc	AMET293826:AMET_2458-MONOMER.
Family and domain databases
HAMAP	MF_00036_B. Ala_tRNA_synth_B. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR023033. Ala_tRNA_synth_euk/bac. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
KO	K01872.
PANTHER	PTHR11777:SF6. PTHR11777:SF6. 1 hit.
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00344. AlaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_ALKMQ

Accession

Primary (citable) accession number: A6TQZ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	September 2, 2008
Last modified:	January 25, 2012
This is version 31 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program