Reviewed, UniProtKB/Swiss-Prot A6TNP8 (SYFA_ALKMQ)

Names and origin

Protein names

Recommended name:
    Phenylalanyl-tRNA synthetase alpha chain
    EC=6.1.1.20
Alternative name(s):
    Phenylalanine--tRNA ligase alpha chain
      Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	Amet_1639

Organism

Alkaliphilus metalliredigens (strain QYMF) [Complete proteome] [HAMAP]

Taxonomic identifier

293826 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity.
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00281
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	Phenylalanyl-tRNA synthetase alpha chain HAMAP MF_00281		PRO_1000059233
Sites
	Metal binding	254	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6TNP8-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 460921578254C4D1	FASTA	339	38,145
	10 20 30 40 50 60 MEAKLKQLEE KAKQDIQAST STDSLEKVRV KYLGKKGELT EVLRGMGSLS KEERPLVGKI 70 80 90 100 110 120 ANLVREQIEE ALELAKGAVK DKELESKLKM ESLDVTMPGK TREIGKRHPL IQAMDELKSI 130 140 150 160 170 180 FIGMGFKVAE GPEVETVHYN FDALNAAPNH PSRDMSDTFY INDHIILRTQ TSPVQVRTME 190 200 210 220 230 240 LQKPPIRIIS PGRCFRNDTP DATHTPMFHQ LEGLVVDKGI TLGDLKGTLE VFIKQFFGND 250 260 270 280 290 300 TKVKFRPHHF PFTEPSAEVD VTCFKCAGHG CSMCKGEGWI ELLGAGMVHP NVLRNCGIDP 310 320 330 EIYSGFAFGM GIDRLTMAKY EIDDIRLLFE NDMRFINQF

References

[1]

"Complete sequence of Alkaliphilus metalliredigens QYMF." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Ye Q., Zhou J., Fields M., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000724 Genomic DNA. Translation: ABR47816.1.
RefSeq	YP_001319475.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6TNP8.
Genome annotation databases
GeneID	5311693.
GenomeReviews	Gene locus Amet_1639 in contig CP000724_GR.
KEGG	amt:Amet_1639.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	FRASYFP.
Family and domain databases
HAMAP	MF_00281. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II_cons-reg. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR002319. Phenylalanyl-tRNA_Synthase_acu. [Graphical view]
PANTHER	PTHR11538. tRNA-synt_2d. 1 hit.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
TIGRFAMs	TIGR00468. pheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_ALKMQ

Accession

Primary (citable) accession number: A6TNP8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	August 21, 2007
Last modified:	November 3, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)