A6TNI2 (KATG1_ALKMQ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase 1
Short name=CP 1
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase 1

Gene names

Name:	katG1
Ordered Locus Names:	Amet_1570

Organism

Alkaliphilus metalliredigens (strain QYMF) [Complete proteome] [HAMAP]

Taxonomic identifier

293826 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus

Protein attributes

Sequence length	730 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 730	730	Catalase-peroxidase 1 HAMAP MF_01961		PRO_0000354718
Sites
	Active site	96	1	Proton acceptor By similarity
	Metal binding	259	1	Iron (heme axial ligand) By similarity
	Site	92	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	95 ↔ 218		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-244) By similarity
	Cross-link	218 ↔ 244		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-95) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6TNI2 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 4B07364EE570C052	FASTA	730	80,734
	10 20 30 40 50 60 MQEKGKCPVT GMTKHKTSGG TTNQDWWPNQ LNLKMLHQNP TMRNPMGERF NYAEEFKKLD 70 80 90 100 110 120 FEALKNDLYA LMTDSQEWWP ADYGHYGPLF IRMAWHSAGT YRMGDGRGGA GTGNQRFAPL 130 140 150 160 170 180 NSWPDNVNLD KARRLLWPIK QKYGKKISWA DLMILAGNCA LESMGFKTFG FAGGREDIWE 190 200 210 220 230 240 PEEDIYWGQE NEWLGDKRYS GDRELENPLA AVQMGLIYVN PEGPNGQPSA LASGKDIRDT 250 260 270 280 290 300 FARMAMNDEE TVALVAGGHT FGKCHGAGSA THVGPEPEAA NIEEQGLGWK NSMGSGKGIH 310 320 330 340 350 360 TISSGIEGAW TPTPIKWDNS YLDTLFNYDW DLVKSPAGAW QWVPTDPTAA DSVPDAHDPS 370 380 390 400 410 420 KRHAPMMTTA DLALRMDPIY GPIAKGFREN PEAFADAFGR AWFKLTHRDM GPRTRFLGPE 430 440 450 460 470 480 VPTEELIWQD PVPAVDFELI DEDDITGLKG KIIDSGLSLS ELVSTAWASA SSFRGSDKRG 490 500 510 520 530 540 GANGARIRLA PQKDWEVNQP EQLQTVLQAL EKIQDTFNSA QSGKKRISLA DLIVLAGCAG 550 560 570 580 590 600 VEQAAKNAGF DVCVPFTPGR TDASQEQTEV ESFSVLEPMA DGFRNYSKGK YTISAEKLLV 610 620 630 640 650 660 DRTQLLGLTA PEMTVLVGGM RVLNANYNKS QQGVLTKRPE NLTNDFFVNL LDMDTAWKVT 670 680 690 700 710 720 AEGGDMFEGI DRKTGELKWT GTGVDLVFGS NSELRAIAEV YASDDAKQKF VEDFVTAWNK 730 VMNADRFDLA

References

[1]

"Complete sequence of Alkaliphilus metalliredigens QYMF." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Ye Q., Zhou J., Fields M., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: QYMF.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000724 Genomic DNA. Translation: ABR47750.1.
RefSeq	YP_001319409.1. NC_009633.1.
3D structure databases
ProteinModelPortal	A6TNI2.
SMR	A6TNI2. Positions 20-730.
ModBase	Search...
Protein-protein interaction databases
STRING	A6TNI2.
Protein family/group databases
PeroxiBase	2533. AmeCP01_QYMF.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5311621.
GenomeReviews	Gene locus Amet_1570 in contig CP000724_GR.
KEGG	amt:Amet_1570.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0376.
HOGENOM	HBG285610.
OMA	KRHAPSM.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	AMET293826:AMET_1570-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG1_ALKMQ

Accession

Primary (citable) accession number: A6TNI2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	August 21, 2007
Last modified:	November 16, 2011
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program