Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Alkaliphilus metalliredigens (strain QYMF)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei43S-adenosyl-L-methionineUniRule annotation1
Binding sitei68S-adenosyl-L-methionineUniRule annotation1
Binding sitei99S-adenosyl-L-methionineUniRule annotation1
Active sitei121By similarity1
Binding sitei121S-adenosyl-L-methionineUniRule annotation1
Binding sitei125SubstrateUniRule annotation1
Binding sitei157SubstrateUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:Amet_1206
OrganismiAlkaliphilus metalliredigens (strain QYMF)
Taxonomic identifieri293826 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeAlkaliphilus
Proteomesi
  • UP000001572 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000643851 – 214tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST214

Interactioni

Protein-protein interaction databases

STRINGi293826.Amet_1206.

Structurei

3D structure databases

SMRiA6TMJ6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni194 – 197Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ1. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000251689.
KOiK03439.
OMAiWITFPDP.
OrthoDBiPOG091H015P.

Family and domain databases

HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiView protein in InterPro
IPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
PANTHERiPTHR23417. PTHR23417. 1 hit.
PfamiView protein in Pfam
PF02390. Methyltransf_4. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiView protein in PROSITE
PS51625. SAM_MT_TRMB. 1 hit.

Sequencei

Sequence statusi: Complete.

A6TMJ6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVRHIPGAK EQLKEYEFYI QDPSQYQNQW HLYFQNQHPI HLEVGLGKGQ
60 70 80 90 100
FLTTLAKVHK NINYLGLEKS QEVLLQALKK LDRQVSRQEL SNMGLFHYNA
110 120 130 140 150
LDLNEVFGEG NIEKIYLNFS DPWPKERHKK RRLTHQGFLK IYRGLLSEQG
160 170 180 190 200
EIQIKTDNKA LFEFSLQQLK EENFHFVQVI YNLHNDGIKD PFMTEYEEKF
210
VKAGKTIYKC IATV
Length:214
Mass (Da):25,387
Last modified:August 21, 2007 - v1
Checksum:i26CF3E3A3C29D07E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000724 Genomic DNA. Translation: ABR47414.1.
RefSeqiWP_012062455.1. NC_009633.1.

Genome annotation databases

EnsemblBacteriaiABR47414; ABR47414; Amet_1206.
KEGGiamt:Amet_1206.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiTRMB_ALKMQ
AccessioniPrimary (citable) accession number: A6TMJ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: August 21, 2007
Last modified: June 7, 2017
This is version 63 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families