A6TLS3 (PURL_ALKMQ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	Amet_0921

Organism

Alkaliphilus metalliredigens (strain QYMF) [Complete proteome] [HAMAP]

Taxonomic identifier

293826 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus

Protein attributes

Sequence length	738 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 738	738	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_1000060086
Regions
	Nucleotide binding	98 – 109	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A6TLS3 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 4234B5F813F91FC9	FASTA	738	79,822
	10 20 30 40 50 60 MTNQYEAVGL KAKEYEGIVE MLGREPNELE LNLYGVMWSE HCSYKHSRSM FKHFPTSGPS 70 80 90 100 110 120 VLQGPGENAG IVDIGDGLAI AMKIESHNHP SAIEPYQGAA TGVGGIIRDI FAMGARPIAL 130 140 150 160 170 180 LNSLRFGELE GDARVKYLLE GVVEGIAGYG NCMGIPTVGG EVYFNQSYRG NPLVNAMCVG 190 200 210 220 230 240 LIEHDAIHRG TASGVGNSIM YVGAATGRDG IGGASFASAT LTEESEEKRA AVQVGDPFME 250 260 270 280 290 300 KLLLEACLEL LKTGSIIGIQ DLGAAGLVSA CCETATRGEG GMEIDVLKVP RRETGMVPVE 310 320 330 340 350 360 VMISESQERM LLIVERGREE EVNEIVKKWG LHSVIIGRVT NDDKLRIFEG DKVVGEIPAE 370 380 390 400 410 420 SLDSSGAPRY EPDYAPPADL AELQKLDIES IPEPRDLSST LRKLLASPNI ASKEWIYRQY 430 440 450 460 470 480 DHMVRTNTVI KPGSDAAVLR IRGTKKGIAL TTDCNSRYCY LDPREGSKIA VVEAARNIVC 490 500 510 520 530 540 SGGKPIAITD GLNFGSPETP EGYWQFRESV LGLSEACREM DTPVISGNVS FYNQTEKGSI 550 560 570 580 590 600 HPTPIVGMVG LIEDISKTCT MAFKEAGDII VLLGQTKAEI GGSEYLASIH GQEKGKIPHL 610 620 630 640 650 660 NLSLEKRLQK EVLALIQGDL VQSAHDLSEG GLAVGVAECA IAGGIGARVE VNTELRNDIV 670 680 690 700 710 720 LFSESQSRFL MTIKPEHLET VQERLKQSNI PHEQLGTVIG NELQMQINGN VVVKESIGEL 730 EEIWRGALQC LMESMKID

References

[1]

"Complete sequence of Alkaliphilus metalliredigens QYMF." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Ye Q., Zhou J., Fields M., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: QYMF.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000724 Genomic DNA. Translation: ABR47141.1.
RefSeq	YP_001318800.1. NC_009633.1.
3D structure databases
ProteinModelPortal	A6TLS3.
ModBase	Search...
Protein-protein interaction databases
STRING	A6TLS3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5310964.
GenomeReviews	Gene locus Amet_0921 in contig CP000724_GR.
KEGG	amt:Amet_0921.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0046.
HOGENOM	HBG311214.
OMA	YGNSFGV.
Enzyme and pathway databases
BioCyc	AMET293826:AMET_0921-MONOMER.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_ALKMQ

Accession

Primary (citable) accession number: A6TLS3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	August 21, 2007
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program