Reviewed, UniProtKB/Swiss-Prot A6TL10 (ASSY_ALKMQ)

Names and origin

Protein names

Recommended name:
    Argininosuccinate synthase
    EC=6.3.4.5
Alternative name(s):
    Citrulline--aspartate ligase

Gene names

Name:	argG
Ordered Locus Names:	Amet_0653

Organism

Alkaliphilus metalliredigens (strain QYMF) [Complete proteome] [HAMAP]

Taxonomic identifier

293826 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus

Protein attributes

Sequence length	414 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP MF_00005
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP MF_00005
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP argininosuccinate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 414	414	Argininosuccinate synthase HAMAP MF_00005		PRO_0000321299
Regions
	Nucleotide binding	12 – 20	9	ATP By similarity
Sites
	Binding site	90	1	Citrulline By similarity
	Binding site	95	1	Citrulline By similarity
	Binding site	120	1	ATP; via amide nitrogen By similarity
	Binding site	122	1	Aspartate By similarity
	Binding site	126	1	Aspartate By similarity
	Binding site	126	1	Citrulline By similarity
	Binding site	127	1	Aspartate By similarity
	Binding site	130	1	Citrulline By similarity
	Binding site	179	1	Citrulline By similarity
	Binding site	188	1	Citrulline By similarity
	Binding site	264	1	Citrulline By similarity
	Binding site	276	1	Citrulline By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6TL10-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 47FCF9714C88AAA9	FASTA	414	45,983
	10 20 30 40 50 60 METKNKKKVV LAYSGGLDTS VILKWLEETY GYEVIAACVN VGQTEDFAAI KKKALATGAS 70 80 90 100 110 120 KAYIVDVTEE FITDYIFPTL KAGAVYEDDY LLGTSFARPL ISKKLVEIAE KEGAVAIAHG 130 140 150 160 170 180 ATGKGNDQVR FEATIKALNP NLKIIAPWRT WDLKSREDCI DYAVQHGIPI PVTKKDIYSR 190 200 210 220 230 240 DENIWHISHE GGNLENPWNE HDDTIYKLSV SPEKSPDTPT YVELEFYKGI PVAVDGVKYE 250 260 270 280 290 300 PIDMLTTLNK LGGAHGVGII DIVENRLVGM KSRGVYETPG GTLLFAAHKA LEKLTLDRDT 310 320 330 340 350 360 TSFKKGISVK YAQLVYDGLW HTPLKDALDQ FVNSTQEFVT GQVKLKLYKG NCTAVASASP 370 380 390 400 410 FSLYNEDFVT FGEDHVYNQQ DAEGFINLFA LPLTIRALML EEKLQGEAKE KKII

References

[1]

"Complete sequence of Alkaliphilus metalliredigens QYMF." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Ye Q., Zhou J., Fields M., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000724 Genomic DNA. Translation: ABR46878.1.
RefSeq	YP_001318537.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6TL10.
Genome annotation databases
GeneID	5310696.
GenomeReviews	Gene locus Amet_0653 in contig CP000724_GR.
KEGG	amt:Amet_0653.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	IAPVREW.
Family and domain databases
HAMAP	MF_00005. [Tree]
InterPro	IPR001518. Arginosuc_synth. IPR018223. Arginosuc_synth_CS. [Graphical view]
PANTHER	PTHR11587. Arginosuc_synth. 1 hit.
Pfam	PF00764. Arginosuc_synth. 1 hit. [Graphical view]
TIGRFAMs	TIGR00032. argG. 1 hit.
PROSITE	PS00564. ARGININOSUCCIN_SYN_1. 1 hit. PS00565. ARGININOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASSY_ALKMQ

Accession

Primary (citable) accession number: A6TL10

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	August 21, 2007
Last modified:	November 3, 2009
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)