Reviewed, UniProtKB/Swiss-Prot A6TKR6 (GLPK_ALKMQ)

Names and origin

Protein names

Recommended name:
    Glycerol kinase
    EC=2.7.1.30
Alternative name(s):
    ATP:glycerol 3-phosphotransferase
    Glycerokinase
      Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	Amet_0557

Organism

Alkaliphilus metalliredigens (strain QYMF) [Complete proteome] [HAMAP]

Taxonomic identifier

293826 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glycerol kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 500	500	Glycerol kinase HAMAP MF_00186		PRO_1000058442
Regions
	Nucleotide binding	410 – 414	5	ATP By similarity
Sites
	Binding site	12	1	Substrate By similarity
	Binding site	16	1	ATP By similarity
	Binding site	82	1	Substrate By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	244	1	Substrate By similarity
	Binding site	266	1	ATP By similarity
	Binding site	309	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6TKR6-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 8A96237AB21AF8AB	FASTA	500	55,606
	10 20 30 40 50 60 MKKYIMALDQ GTTSSRAILF DQEGKRVGSS QKEFTQFYPK AGWVEHDPME IWGTQSGVAR 70 80 90 100 110 120 EVLETTGIST QDIAAIGITN QRETTIIWDK NTGKPIYNAI VWQCRRTAGI CDELKAQGME 130 140 150 160 170 180 TYIRENTGLV VDAYFSGTKV KWILDHVEGA REKAENGELL FGTVDSWLIW NLTRGKVHVT 190 200 210 220 230 240 DYSNASRTML YNIKELKWDE KILEALDIPK SMLPEVKASS EVYGHTDHQT FGGADIPIAG 250 260 270 280 290 300 AAGDQQAALF GQACFQPGMA KNTYGTGCFM LMNTGEKFVP SENGLLTTLA WGVDGKVEYA 310 320 330 340 350 360 LEGSIFVAGA AVQWLRDELR IIRDAEDTEY LATKVADSNG VYVVPAFTGM GAPYWDMYAR 370 380 390 400 410 420 GAILGLTRGA KAEHIIRATL ESIAYQTRDV LEAMQEDSGI ELRSLKVDGG AVSNNFLMQF 430 440 450 460 470 480 QADILGVQVD RPEIIETTAL GAAYLAGLAV GFWRDKNEIA NKWKVDTVFS PTMENTKKEK 490 500 MYRGWKRAVN RALKWELEEE

References

[1]

"Complete sequence of Alkaliphilus metalliredigens QYMF." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Ye Q., Zhou J., Fields M., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000724 Genomic DNA. Translation: ABR46784.1.
RefSeq	YP_001318443.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6TKR6.
Genome annotation databases
GeneID	5310600.
GenomeReviews	Gene locus Amet_0557 in contig CP000724_GR.
KEGG	amt:Amet_0557.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	IAQREFT.
Family and domain databases
HAMAP	MF_00186. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_ALKMQ

Accession

Primary (citable) accession number: A6TKR6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	August 21, 2007
Last modified:	November 3, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)