Reviewed, UniProtKB/Swiss-Prot A6TJM2 (MURC_ALKMQ)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramate--L-alanine ligase
    EC=6.3.2.8
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	Amet_0153

Organism

Alkaliphilus metalliredigens (strain QYMF) [Complete proteome] [HAMAP]

Taxonomic identifier

293826 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus

Protein attributes

Sequence length	460 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 460	460	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_0000336810
Regions
	Nucleotide binding	119 – 125	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A6TJM2-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 10A91472428BA0B6	FASTA	460	51,464
	10 20 30 40 50 60 MIAFNMDEHQ LKHIHLIGIG GISMSAIAEI LLENGYHISG SDMKESNLTH KLRDHGAEIF 70 80 90 100 110 120 IGHASENIQN PDLVVYTAAV KADNPERIRA EELGIPLADR AEMLGQIMKK YEKAIAVAGS 130 140 150 160 170 180 HGKTTTTSLI SLLMEYSNLD PTILVGGELD EIGGNIKIGQ SQHFITEACE YVESFLKFYP 190 200 210 220 230 240 FIGIILNIDE DHLDYFKDIE HIKSAFKKFA QRIPKEGFLI ASYDDAHVRE ISRDLDCHVI 250 260 270 280 290 300 TYGIKTKSQF MAHNIEFSFE GLPSFDVSFE GKTIGSFSLN IPGLHNVYNS LAAIATTYVL 310 320 330 340 350 360 GVDPVAISKH ITRFKGIHRR FDLLGEVKGA KVIDDYAHHP VEIRATLEAA KKYPHKKIWC 370 380 390 400 410 420 VFQPHTYSRT QALLKDFAKS FYLADHVIIT DIYAAREKDE GIVNSQGLVN LIVQEHPAQY 430 440 450 460 IGGFEEISRY LYDHIEAGDI VLTMGAGDVY LIAQMLLAEK

References

[1]

"Complete sequence of Alkaliphilus metalliredigens QYMF." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Ye Q., Zhou J., Fields M., Richardson P. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000724 Genomic DNA. Translation: ABR46390.1.
RefSeq	YP_001318049.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6TJM2.
Genome annotation databases
GeneID	5310196.
GenomeReviews	Gene locus Amet_0153 in contig CP000724_GR.
KEGG	amt:Amet_0153.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	EWMVVEA.
Family and domain databases
HAMAP	MF_00046. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01082. murC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_ALKMQ

Accession

Primary (citable) accession number: A6TJM2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	August 21, 2007
Last modified:	November 3, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)