Reviewed, UniProtKB/Swiss-Prot A6TH46 (DSBD_KLEP7)

Names and origin

Protein names

Recommended name:
    Thiol:disulfide interchange protein dsbD
    EC=1.8.1.8
Alternative name(s):
    Protein-disulfide reductase
      Short name=Disulfide reductase

Gene names

Name:	dsbD
Ordered Locus Names:	KPN78578_44560
ORF Names:	KPN_04526

Organism

Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578) [Complete proteome] [HAMAP]

Taxonomic identifier

272620 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	598 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Required to facilitate the formation of correct disulfide bonds in some periplasmic proteins and for the assembly of the periplasmic c-type cytochromes. Acts by transferring electrons from cytoplasmic thioredoxin to the periplasm. This transfer involves a cascade of disulfide bond formation and reduction steps By similarity.
Catalytic activity	Protein dithiol + NAD(P)+ = protein disulfide + NAD(P)H. HAMAP MF_00399
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the thioredoxin family. DsbD subfamily. Contains 1 thioredoxin domain.

Ontologies

Keywords
Biological process	Cytochrome c-type biogenesis Electron transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Redox-active center Signal Transmembrane
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro cytochrome complex assembly Inferred from electronic annotation. Source: HAMAP electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: HAMAP protein-disulfide reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 598	579	Thiol:disulfide interchange protein dsbD HAMAP MF_00399		PRO_1000049611
Regions
	Transmembrane	197 – 217	21	Potential
	Transmembrane	242 – 262	21	Potential
	Transmembrane	277 – 297	21	Potential
	Transmembrane	330 – 350	21	Potential
	Transmembrane	356 – 376	21	Potential
	Transmembrane	391 – 411	21	Potential
	Transmembrane	423 – 443	21	Potential
	Domain	459 – 598	140	Thioredoxin
Amino acid modifications
	Disulfide bond	122 ↔ 128		Redox-active By similarity
	Disulfide bond	216 ↔ 338		Redox-active By similarity
	Disulfide bond	513 ↔ 516		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6TH46-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: E32B174BD62D1075	FASTA	598	64,599
	10 20 30 40 50 60 MAYRIITLIL LLCSTSATAG LFDAPGRSNF VPADQAFAFD FQQQQHDVNL SWQIKDGYYL 70 80 90 100 110 120 YRQQFTFSAA GATIDEPALP AGEWHEDEFY GKSEIFRQRL TVPVTVKEAD KEATLTVTWQ 130 140 150 160 170 180 GCADAGFCYP PETKVIPLSA VRAASNDGQA TAIEPMPSTS SRPAFNPPLP VEPRPAPELA 190 200 210 220 230 240 TSPAPAAVPP ADTPARLPFT ALWALLIGIG IAFTPCVLPM YPLISGIVLG GKQRLSTARA 250 260 270 280 290 300 LLLAFIYVQG MALTYTALGL VVAAAGLQFQ AALQHPYVLV GLSAVFILLA LSMFGLFTLQ 310 320 330 340 350 360 LPSSLQTRLT LLSNKRQGGS PGGVFAMGAI AGLICSPCTT APLSAILLYI AQSGNLWLGG 370 380 390 400 410 420 GTLYLYALGM GLPLILMTVF GNRLLPKSGP WMSHVKTAFG FVILALPVFL LERILGDQWG 430 440 450 460 470 480 LRLWSMLGVA FFSWAFITSL GATRPWMRLV QIILLAAALV SARPLQDWAF GAPAVEQQAH 490 500 510 520 530 540 LAFTRVSSVA ELDQALAQAK GQPVMLDLYA DWCVACKEFE KYTFSSPDVQ QALKGTVLLQ 550 560 570 580 590 VDVTKNSPQD VALLKHLQVL GLPTILFFNA EGQEQSERRV TGFMDAAAFS AHLRDWQA

References

[1]	McClelland M., Sanderson E.K., Spieth J., Clifton W.S., Latreille P., Sabo A., Pepin K., Bhonagiri V., Porwollik S., Ali J., Wilson R.K. Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000647 Genomic DNA. Translation: ABR79880.1.
RefSeq	YP_001338147.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5339983.
GenomeReviews	Gene locus KPN78578_44560 in contig CP000647_GR.
KEGG	kpn:KPN_04526.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A6TH46. HTDEYFG.
Family and domain databases
HAMAP	MF_00399. [Tree]
InterPro	IPR003834. Cyt_c_assmbl_TM. IPR017936. Thioredoxin-like. IPR017937. Thioredoxin_CS. IPR013766. Thioredoxin_domain. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF02683. DsbD. 1 hit. PF00085. Thioredoxin. 1 hit. [Graphical view]
PROSITE	PS00194. THIOREDOXIN_1. 1 hit. PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DSBD_KLEP7

Accession

Primary (citable) accession number: A6TH46

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 21, 2007
Last modified:	June 16, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)