A6TGR8 (ACEK_KLEP7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isocitrate dehydrogenase kinase/phosphatase
Short name=IDH kinase/phosphatase
Short name=IDHK/P
EC=2.7.11.5
EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	KPN78578_43280
ORF Names:	KPN_04397

Organism

Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578) [Complete proteome] [HAMAP]

Taxonomic identifier

272620 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	594 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity. HAMAP MF_00747
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity HAMAP MF_00747.
Sequence similarities	Belongs to the AceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: EC phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 594	594	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_1000046553
Regions
	Nucleotide binding	315 – 321	7	ATP By similarity
Sites
	Active site	371	1	By similarity
	Binding site	336	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6TGR8 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 6C31D8D0B4A1D401	FASTA	594	68,844
	10 20 30 40 50 60 MTRGLELLIA QTILQGFDAQ YGRFLEVTSG AQQRFEQADW HAVQQAMKQR IHLYDHHVGL 70 80 90 100 110 120 VVEQLRCITE GKSTDVDFLL RVKQQYTQLL PDYPRFEIAE SFFNSVYCRL FDHRSLTPER 130 140 150 160 170 180 LFIFSSQPER PFRTLPRPLA KDFFPERGWS HLLGKVLSDL PLRLPWQNKA RDIGYIIASL 190 200 210 220 230 240 QEALGEELLA TCHLQVANEL FYRNKAAWLV GKLVMPMATL PFLLPIHRSE EGELFVDTCL 250 260 270 280 290 300 TTHAEASIVF GFARSYFMVY APLPGALVEW LREILPGKTT AELYMAIGCQ KHAKTESYRE 310 320 330 340 350 360 YLHYITRCDE QFIEAPGIRG MVMLVFTLPG FDRVFKVIKD RFAPQKEMTA AHVRACYQLV 370 380 390 400 410 420 KEHDRVGRMA DTQEFENFVL DKRQIAPALL ALLQAEAGNK LTDLGDRIVI SHLYIERRMV 430 440 450 460 470 480 PLNLWLEQVN GQALRDAVEE YGNAIRQLAA ANIFPGDMLF KNFGVTRHGR VVFYDYDEIC 490 500 510 520 530 540 YMTEVNFREI PPPRYPEDEL ASEPWYSVSP GDVFPEEFRH WLCADPRIGP LFEEMHADLL 550 560 570 580 590 RADYWRALQM RIKNGHVEDV YAYRRKQRFS VRYGADSRPD KAFTPPSGKV RRSA

References

[1]

The Klebsiella pneumonia Genome Sequencing Project McClelland M., Sanderson E.K., Spieth J., Clifton W.S., Latreille P., Sabo A., Pepin K., Bhonagiri V., Porwollik S., Ali J., Wilson R.K. Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700721 / MGH 78578.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000647 Genomic DNA. Translation: ABR79752.1.
RefSeq	YP_001338019.1. NC_009648.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6TGR8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5338227.
GenomeReviews	Gene locus KPN78578_43280 in contig CP000647_GR.
KEGG	kpn:KPN_04397.
PATRIC	20463254. VBIKlePne13394_4441.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG4579.
HOGENOM	HBG298593.
OMA	RMTPLNI.
ProtClustDB	PRK02946.
Family and domain databases
HAMAP	MF_00747. AceK. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
KO	K00906.
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProDom	PD043552. Isocitrate_DH_AceK. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

ACEK_KLEP7

Accession

Primary (citable) accession number: A6TGR8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 21, 2007
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program