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A6TGE9 (TRMA_KLEP7) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA (uracil(54)-C(5))-methyltransferase
EC=2.1.1.35
Alternative name(s):
tRNA(m5U54)-methyltransferase
Short name=RUMT
Gene names
Name:trmA
Ordered Locus Names:KPN78578_42090
ORF Names:KPN_04254
OrganismKlebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578) [Complete proteome] [HAMAP]
Taxonomic identifier272620 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella ›

Protein attributes

Sequence length366 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of 5-methyl-uridine at position 54 (m5U54) in all tRNAs By similarity. HAMAP-Rule MF_01011

Catalytic activity

S-adenosyl-L-methionine + uridine54 in tRNA = S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA. HAMAP-Rule MF_01011

Sequence similarities

Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. TrmA subfamily.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functionS-adenosylmethionine-dependent tRNA (m5U54) methyltransferase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 366366tRNA (uracil(54)-C(5))-methyltransferase HAMAP-Rule MF_01011
PRO_1000072910

Sites

Active site3241Nucleophile By similarity
Binding site1901S-adenosyl-L-methionine By similarity
Binding site2181S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site2231S-adenosyl-L-methionine By similarity
Binding site2391S-adenosyl-L-methionine By similarity
Binding site2991S-adenosyl-L-methionine By similarity

Sequences

Sequence LengthMass (Da)Tools
A6TGE9 [UniParc].

Last modified August 21, 2007. Version 1.
Checksum: 76BE7481F1F70899

FASTA36641,845
        10         20         30         40         50         60 
MTPEHLPTEQ YEAQLAEKVV RLQTMMAPFA APVPEVFRSP VSHYRMRAEF RLWHDGDDLY 

        70         80         90        100        110        120 
HIIFDQQTRS RIRVDSFPAA SALINQLMTA MLEGVRNNPV LRQKLFQIDY LTTLSNQAVV 

       130        140        150        160        170        180 
SVLYHKKLDD AWREQAEALR DALRAQGLNV HLIGRATKTK IELDQDYIDE RLPVGGREMI 

       190        200        210        220        230        240 
YRQVENSFTQ PNAAMNIQML EWALDVTKGA TGDLLELYCG NGNFSLALAR NFDRVLATEI 

       250        260        270        280        290        300 
AKPSVAAAQY NIAANHIDNV QIIRMAAEEF TQAMNGVRQF NRLQGIDLHS YQCETIFVDP 

       310        320        330        340        350        360 
PRSGLDSETE KMVQAYPRIL YISCNPETLC RNLETLSQTH NVTRLALFDQ FPYTHHMECG 


VLLTRK

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References

[1]The Klebsiella pneumonia Genome Sequencing Project
McClelland M., Sanderson E.K., Spieth J., Clifton W.S., Latreille P., Sabo A., Pepin K., Bhonagiri V., Porwollik S., Ali J., Wilson R.K.
Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700721 / MGH 78578.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000647 Genomic DNA. Translation: ABR79633.1.
RefSeqYP_001337900.1. NC_009648.1.

3D structure databases

ProteinModelPortalA6TGE9.
SMRA6TGE9. Positions 1-366.
ModBaseSearch...

Protein-protein interaction databases

STRING272620.KPN_04254.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABR79633; ABR79633; KPN_04254.
GeneID5342032.
KEGGkpn:KPN_04254.
PATRIC20462968. VBIKlePne13394_4322.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2265.
HOGENOMHOG000218626.
KOK00557.
OMAESGVWLI.
ProtClustDBPRK05031.

Enzyme and pathway databases

BioCycKPNE272620:GKDC-4291-MONOMER.

Family and domain databases

HAMAPMF_01011. tRNA_methyltr_TrmA.
InterProIPR011869. TrmA_MeTrfase_proteobac.
IPR010280. U5_MeTrfase.
[Graphical view]
PfamPF05958. tRNA_U5-meth_tr. 1 hit.
[Graphical view]
TIGRFAMsTIGR02143. trmA_only. 1 hit.
PROSITEPS01230. TRMA_1. 1 hit.
PS01231. TRMA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMA_KLEP7
AccessionPrimary (citable) accession number: A6TGE9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: August 21, 2007
Last modified: May 29, 2013
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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