Reviewed, UniProtKB/Swiss-Prot A6TGE6 (STHA_KLEP7)

Names and origin

Protein names

Recommended name:
    Soluble pyridine nucleotide transhydrogenase
      Short name=STH
    EC=1.6.1.1
Alternative name(s):
    NAD(P)(+) transhydrogenase [B-specific]

Gene names

Name:	sthA
Synonyms:	udhA
Ordered Locus Names:	KPN78578_42060
ORF Names:	KPN_04251

Organism

Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578) [Complete proteome] [HAMAP]

Taxonomic identifier

272620 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Conversion of NADPH, generated by peripheral catabolic pathways, to NADH, which can enter the respiratory chain for energy generation By similarity. HAMAP MF_00247
Catalytic activity	NADPH + NAD+ = NADP+ + NADH. HAMAP MF_00247
Cofactor	Binds 1 FAD per subunit By similarity. HAMAP MF_00247
Subcellular location	Cytoplasm By similarity HAMAP MF_00247.
Sequence similarities	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NADP metabolic process Inferred from electronic annotation. Source: HAMAP cell redox homeostasis Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NAD(P)+ transhydrogenase (B-specific) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 466	466	Soluble pyridine nucleotide transhydrogenase HAMAP MF_00247		PRO_1000012557
Regions
	Nucleotide binding	36 – 45	10	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6TGE6-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: D792446B10D44AFD	FASTA	466	51,532
	10 20 30 40 50 60 MSHSWDYDAI VIGSGPGGEG AAMGLVKQGA RVAVIERYHN VGGGCTHWGT IPSKALRHAV 70 80 90 100 110 120 SRIIEFNQNP LYSDHSRLLR SSFADILNHA DSVINQQTHM RQGFYERNHC EILQGNAHFV 130 140 150 160 170 180 DEHTLALECH DGTVETVTAE KFVIACGSRP YHPADVDFHH PRIYDSDSIL SLQHEPRHVI 190 200 210 220 230 240 IYGAGVIGCE YASIFRGMEV KVDLINTRDR LLAFLDQEMS DSLSYHFWNS GVVIRHNEEY 250 260 270 280 290 300 EKIEGVDDGV IMHLKSGKKL KADCLLYANG RTGNTDTLAL ENIGLQTDSR GQLKVNSMYQ 310 320 330 340 350 360 TALPHIYAVG DVIGYPSLAS AAYDQGRIAA QALVKGEASA HLIEDIPTGI YTIPEISSVG 370 380 390 400 410 420 KTEQQLTAMK VPYEVGRAQF KHLARAQIVG MSVGTLKILF HRETKEILGI HCFGERAAEI 430 440 450 460 IHIGQAIMEQ KGGGNTIEYF VNTTFNYPTM AEAYRVAALN GLNRLF

References

[1]	McClelland M., Sanderson E.K., Spieth J., Clifton W.S., Latreille P., Sabo A., Pepin K., Bhonagiri V., Porwollik S., Ali J., Wilson R.K. Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000647 Genomic DNA. Translation: ABR79630.1.
RefSeq	YP_001337897.1.
3D structure databases
SMR	A6TGE6. Positions 7-464.
ModBase	Search...
Protein-protein interaction databases
STRING	A6TGE6.
Genome annotation databases
GeneID	5339670.
GenomeReviews	Gene locus KPN78578_42060 in contig CP000647_GR.
KEGG	kpn:KPN_04251.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1249.
HOGENOM	HBG515043.
OMA	RDIGEPR.
Family and domain databases
HAMAP	MF_00247. SthA. [Tree]
InterPro	IPR016156. FAD/NAD-linked_Rdtase_dimer. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR000815. Hg_reductase. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR001327. Pyr_OxRdtase_NAD_bd. [Graphical view]
Gene3D	G3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF02852. Pyr_redox_dim. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00945. HGRDTASE.
ProtoNet	Search...

Entry information

Entry name

STHA_KLEP7

Accession

Primary (citable) accession number: A6TGE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 21, 2007
Last modified:	February 9, 2010
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)