Reviewed, UniProtKB/Swiss-Prot A6TG48 (ASNA_KLEP7)

Names and origin

Protein names

Recommended name:
    Aspartate--ammonia ligase
    EC=6.3.1.1
Alternative name(s):
    Asparagine synthetase A

Gene names

Name:	asnA
Ordered Locus Names:	KPN78578_41080
ORF Names:	KPN_04149

Organism

Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578) [Complete proteome] [HAMAP]

Taxonomic identifier

272620 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555
Subcellular location	Cytoplasm By similarity HAMAP MF_00555.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: HAMAP tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Aspartate--ammonia ligase HAMAP MF_00555		PRO_1000017949

Sequences

	Sequence		Length	Mass (Da)
	A6TG48-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 376DCE5240EA557D	FASTA	330	36,812
	10 20 30 40 50 60 MKTAYIAKQR QISFVKSHFS RQLEEKLGLI EVQAPILSRV GDGTQDNLSG CEKAVQVKVK 70 80 90 100 110 120 TLPDAQFEVV HSLAKWKRQT LGQHDFSAGE GLYTHMKALR PDEDRLTPIH SVYVDQWDWE 130 140 150 160 170 180 RVMGDEERHV GTLKATVEAI YAGIKATELA VSQEFGLTPF LPEQIHFVHS QELLSRYPEL 190 200 210 220 230 240 DAKGRERAIA KELGAVFLIG IGGKLADGKR HDVRAPDYDD WSTEVSEGFA GLNGDILVWN 250 260 270 280 290 300 PVLEDAFEIS SMGIRVDAEA LKRQLALTGD EDRLKLEWHQ ALLRGEMPQT IGGGIGQSRL 310 320 330 TMLLLQLDHI GQVQCGVWPA QVRESVSALL

References

[1]	McClelland M., Sanderson E.K., Spieth J., Clifton W.S., Latreille P., Sabo A., Pepin K., Bhonagiri V., Porwollik S., Ali J., Wilson R.K. Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000647 Genomic DNA. Translation: ABR79532.1.
RefSeq	YP_001337799.1.
3D structure databases
SMR	A6TG48. Positions 4-330.
ModBase	Search...
Protein-protein interaction databases
STRING	A6TG48.
Genome annotation databases
GeneID	5340250.
GenomeReviews	Gene locus KPN78578_41080 in contig CP000647_GR.
KEGG	kpn:KPN_04149.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2502.
HOGENOM	HBG288146.
OMA	LNDNLNG.
Family and domain databases
HAMAP	MF_00555. AsnA. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II_cons-dom. IPR004618. AsnA. [Graphical view]
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
TIGRFAMs	TIGR00669. asnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNA_KLEP7

Accession

Primary (citable) accession number: A6TG48

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 21, 2007
Last modified:	February 9, 2010
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)