Reviewed, UniProtKB/Swiss-Prot A6TFG7 (GHRB_KLEP7)

Names and origin

Protein names

Recommended name:
    Glyoxylate/hydroxypyruvate reductase B
    EC=1.1.1.79
    EC=1.1.1.81

Gene names

Name:	ghrB
Ordered Locus Names:	KPN78578_38770
ORF Names:	KPN_03915

Organism

Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578) [Complete proteome] [HAMAP]

Taxonomic identifier

272620 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively By similarity.
Catalytic activity	Glycolate + NADP+ = glyoxylate + NADPH. HAMAP MF_01667 D-glycerate + NAD(P)+ = hydroxypyruvate + NAD(P)H. HAMAP MF_01667
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrB subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro glyoxylate reductase (NADP) activity Inferred from electronic annotation. Source: HAMAP hydroxypyruvate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 323	323	Glyoxylate/hydroxypyruvate reductase B HAMAP MF_01667		PRO_0000348392
Sites
	Active site	237	1	By similarity
	Active site	266	1	By similarity
	Active site	285	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6TFG7-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 27C85343A346EDE5	FASTA	323	35,433
	10 20 30 40 50 60 MKPSVILYKT LPDDLLQRLE EHFSVTQVKN LRPETVSQHA EAFAQAEGLL GSSEKVDAAL 70 80 90 100 110 120 LEKMPKLRAT STVSVGYDNF DVEALNARRV LLMHTPTVLT ETVADTVMAL VLSTARRVVE 130 140 150 160 170 180 VAERVKAGEW TKSIGPDWFG TDVHHKTLGI VGMGRIGMAL AQRAHFGFGM PILYNARRQH 190 200 210 220 230 240 PQAEERFNAR YCDLDTLLQE ADFVCLILPL SEETHHLFGQ AQFAKMKSSA IFINAGRGPV 250 260 270 280 290 300 VDEQALIAAL QNGEIHAAGL DVFEHEPLAK DSPLLSLPNV VALPHIGSAT HETRYNMAAC 310 320 AVDNLIDALN GNVEKNCVNP QVK

References

[1]	McClelland M., Sanderson E.K., Spieth J., Clifton W.S., Latreille P., Sabo A., Pepin K., Bhonagiri V., Porwollik S., Ali J., Wilson R.K. Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000647 Genomic DNA. Translation: ABR79301.1.
RefSeq	YP_001337568.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5341612.
GenomeReviews	Gene locus KPN78578_38770 in contig CP000647_GR.
KEGG	kpn:KPN_03915.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A6TFG7. KVYIAEP.
Family and domain databases
HAMAP	MF_01667. [Tree]
InterPro	IPR006139. D-isomer_2_OHA_DH. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. False negative. PS00670. D_2_HYDROXYACID_DH_2. 1 hit. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GHRB_KLEP7

Accession

Primary (citable) accession number: A6TFG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	August 21, 2007
Last modified:	June 16, 2009
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)