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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathway:iglycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei405 – 4051NucleophileUniRule annotation
Active sitei458 – 4581Proton donorUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciKPNE272620:GKDC-3835-MONOMER.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
Alpha-(1->4)-glucan branching enzymeUniRule annotation
Glycogen branching enzymeUniRule annotation
Short name:
BEUniRule annotation
Gene namesi
Name:glgBUniRule annotation
Ordered Locus Names:KPN78578_37610
ORF Names:KPN_03798
OrganismiKlebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
Taxonomic identifieri272620 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella
ProteomesiUP000000265 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7287281,4-alpha-glucan branching enzyme GlgBPRO_1000044981Add
BLAST

Proteomic databases

PRIDEiA6TF51.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272620.KPN_03798.

Structurei

3D structure databases

ProteinModelPortaliA6TF51.
SMRiA6TF51. Positions 117-728.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiDATEVWV.
OrthoDBiEOG6JX7GT.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

A6TF51-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNHIDRDVI NALIAGHFAD PFSVLGMHRT DAGLEVRALL PDATDVWVIE
60 70 80 90 100
PKTGRKVGKL ECLDSRGFFS GVLPRRKNAF RYQLAVTWHG QQNLIDDPYR
110 120 130 140 150
FGPLLQDLDV WLLSEGTHLR PYETLGAHAA TMDGVTGTRF SVWAPNARRV
160 170 180 190 200
SVVGQFNYWD GRRHPMRFRK ESGIWELFVP GAHNGQLYKF ELIDAHGNLR
210 220 230 240 250
VKADPYAFES QMRPESASLI CDLPPKVEQP ADRRAANQFD APISIYEVHL
260 270 280 290 300
GSWRRHTDNN FWLSYRELAD QLVPYAKWMG FTHLELLPVN EHPFDGSWGY
310 320 330 340 350
QPTGLYAPTR RFGTRDDFRY FINAAHAAGL NVILDWVPGH FPADDFALAS
360 370 380 390 400
FDGTSLYEHS DPREGYHQDW NTLIYNYGRR EVSNYLVGNA LYWIERFGID
410 420 430 440 450
ALRVDAVASM IYRDYSRKAG EWIPNEYGGR ENLEAIEFLR NTNRILGEQT
460 470 480 490 500
PGAVTMAEES TDFAGVTRPP AGGGLGFWFK WNLGWMHDTL DYMKLDPVHR
510 520 530 540 550
RYHHDKMTFG MLYNYTENFV LPLSHDEVVH GKKSILDRMP GDAWQKFANL
560 570 580 590 600
RAYYGWLFAF PGKKLLFMGN EFAQGREWNH DVSLDWHLLE GGDNWHHGVQ
610 620 630 640 650
RLVRDLNHTY RHHKALHELD FDPYGFEWLV VDDHERSVFV FVRRDRAGNE
660 670 680 690 700
IIVASNFTPV PRHDYRFGIN QPGRWREALN TDSMHYHGSN QGNGGVVESD
710 720
AIASHGREHS LSLTLPPLAT IWLVREAQ
Length:728
Mass (Da):83,824
Last modified:August 21, 2007 - v1
Checksum:i94FAE0FC016E8DC1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000647 Genomic DNA. Translation: ABR79185.1.
RefSeqiWP_004150069.1. NC_009648.1.

Genome annotation databases

EnsemblBacteriaiABR79185; ABR79185; KPN_03798.
KEGGikpn:KPN_03798.
PATRICi20462025. VBIKlePne13394_3857.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000647 Genomic DNA. Translation: ABR79185.1.
RefSeqiWP_004150069.1. NC_009648.1.

3D structure databases

ProteinModelPortaliA6TF51.
SMRiA6TF51. Positions 117-728.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272620.KPN_03798.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Proteomic databases

PRIDEiA6TF51.

Protocols and materials databases

DNASUi5340121.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABR79185; ABR79185; KPN_03798.
KEGGikpn:KPN_03798.
PATRICi20462025. VBIKlePne13394_3857.

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiDATEVWV.
OrthoDBiEOG6JX7GT.

Enzyme and pathway databases

UniPathwayiUPA00164.
BioCyciKPNE272620:GKDC-3835-MONOMER.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700721 / MGH 78578.

Entry informationi

Entry nameiGLGB_KLEP7
AccessioniPrimary (citable) accession number: A6TF51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 21, 2007
Last modified: July 22, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.