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Protein

Malate dehydrogenase

Gene

mdh

Organism
Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.By similarity

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei34 – 341NADBy similarity
Binding sitei81 – 811SubstrateBy similarity
Binding sitei87 – 871SubstrateBy similarity
Binding sitei94 – 941NADBy similarity
Binding sitei119 – 1191SubstrateBy similarity
Binding sitei153 – 1531SubstrateBy similarity
Active sitei177 – 1771Proton acceptorBy similarity
Binding sitei227 – 2271NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi7 – 137NADBy similarity
Nucleotide bindingi117 – 1193NADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciKPNE272620:GKDC-3681-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase (EC:1.1.1.37)
Gene namesi
Name:mdh
Ordered Locus Names:KPN78578_36130
ORF Names:KPN_03644
OrganismiKlebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
Taxonomic identifieri272620 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella
ProteomesiUP000000265 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 312312Malate dehydrogenasePRO_0000313532Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi272620.KPN_03644.

Structurei

3D structure databases

ProteinModelPortaliA6TEQ3.
SMRiA6TEQ3. Positions 1-312.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000213792.
KOiK00024.
OMAiTECTYFS.
OrthoDBiEOG6091FG.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01516. Malate_dehydrog_1.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR023958. Malate_DH_type1_bac.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6TEQ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVAVLGAAG GIGQALALLL KTQLPSGSEL SLYDIAPVTP GVAVDLSHIP
60 70 80 90 100
TDVKIKGFSG EDATPALEGA DVVLISAGVA RKPGMDRSDL FNVNAGIVKN
110 120 130 140 150
LVQQIAKTCP QACIGIITNP VNTTVAIAAE VLKKAGVYDK NKLFGVTTLD
160 170 180 190 200
IIRSNTFVAE LKGKSATEVE VPVIGGHSGV TILPLLSQIP GVSFSDQEIA
210 220 230 240 250
DLTKRIQNAG TEVVEAKAGG GSATLSMGQA AARFGLSLVR AMQGEKGVVE
260 270 280 290 300
CAYVEGDGHY ARFFSQPLLL GKNGVEERQS IGKLSAFEQQ ALEGMLDTLK
310
KDIALGEDFV NK
Length:312
Mass (Da):32,426
Last modified:August 21, 2007 - v1
Checksum:iB33508066CD1FCFB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000647 Genomic DNA. Translation: ABR79037.1.
AJ635379 Genomic DNA. Translation: CAG25790.1.
RefSeqiWP_002918570.1. NC_009648.1.
YP_001337304.1. NC_009648.1.

Genome annotation databases

EnsemblBacteriaiABR79037; ABR79037; KPN_03644.
KEGGikpn:KPN_03644.
PATRICi20461713. VBIKlePne13394_3706.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000647 Genomic DNA. Translation: ABR79037.1.
AJ635379 Genomic DNA. Translation: CAG25790.1.
RefSeqiWP_002918570.1. NC_009648.1.
YP_001337304.1. NC_009648.1.

3D structure databases

ProteinModelPortaliA6TEQ3.
SMRiA6TEQ3. Positions 1-312.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272620.KPN_03644.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABR79037; ABR79037; KPN_03644.
KEGGikpn:KPN_03644.
PATRICi20461713. VBIKlePne13394_3706.

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000213792.
KOiK00024.
OMAiTECTYFS.
OrthoDBiEOG6091FG.

Enzyme and pathway databases

BioCyciKPNE272620:GKDC-3681-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01516. Malate_dehydrog_1.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR023958. Malate_DH_type1_bac.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700721 / MGH 78578.
  2. "Diversity and evolution of the class A chromosomal beta-lactamase gene in Klebsiella pneumoniae."
    Haeggman S., Loefdahl S., Paauw A., Verhoef J., Brisse S.
    Antimicrob. Agents Chemother. 48:2400-2408(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 31-144.

Entry informationi

Entry nameiMDH_KLEP7
AccessioniPrimary (citable) accession number: A6TEQ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 21, 2007
Last modified: May 27, 2015
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.