A6TEQ3 (MDH_KLEP7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate dehydrogenase
EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	KPN78578_36130
ORF Names:	KPN_03644

Organism

Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578) [Complete proteome] [HAMAP]

Taxonomic identifier

272620 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella › Klebsiella pneumoniae › Klebsiella pneumoniae subsp. pneumoniae

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_01516
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_01516
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_01516
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 1 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-HAMAP
Molecular_function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: UniProtKB-HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	Malate dehydrogenase HAMAP-Rule MF_01516		PRO_0000313532
Regions
	Nucleotide binding	7 – 13	7	NAD By similarity
	Nucleotide binding	117 – 119	3	NAD By similarity
Sites
	Active site	177	1	Proton acceptor By similarity
	Binding site	34	1	NAD By similarity
	Binding site	81	1	Substrate By similarity
	Binding site	87	1	Substrate By similarity
	Binding site	94	1	NAD By similarity
	Binding site	119	1	Substrate By similarity
	Binding site	153	1	Substrate By similarity
	Binding site	227	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6TEQ3 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: B33508066CD1FCFB	FASTA	312	32,426
	10 20 30 40 50 60 MKVAVLGAAG GIGQALALLL KTQLPSGSEL SLYDIAPVTP GVAVDLSHIP TDVKIKGFSG 70 80 90 100 110 120 EDATPALEGA DVVLISAGVA RKPGMDRSDL FNVNAGIVKN LVQQIAKTCP QACIGIITNP 130 140 150 160 170 180 VNTTVAIAAE VLKKAGVYDK NKLFGVTTLD IIRSNTFVAE LKGKSATEVE VPVIGGHSGV 190 200 210 220 230 240 TILPLLSQIP GVSFSDQEIA DLTKRIQNAG TEVVEAKAGG GSATLSMGQA AARFGLSLVR 250 260 270 280 290 300 AMQGEKGVVE CAYVEGDGHY ARFFSQPLLL GKNGVEERQS IGKLSAFEQQ ALEGMLDTLK 310 KDIALGEDFV NK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	The Klebsiella pneumonia Genome Sequencing Project McClelland M., Sanderson E.K., Spieth J., Clifton W.S., Latreille P., Sabo A., Pepin K., Bhonagiri V., Porwollik S., Ali J., Wilson R.K. Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700721 / MGH 78578.
[2]	"Diversity and evolution of the class A chromosomal beta-lactamase gene in Klebsiella pneumoniae." Haeggman S., Loefdahl S., Paauw A., Verhoef J., Brisse S. Antimicrob. Agents Chemother. 48:2400-2408(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 31-144.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000647 Genomic DNA. Translation: ABR79037.1. AJ635379 Genomic DNA. Translation: CAG25790.1.
RefSeq	YP_001337304.1. NC_009648.1.
3D structure databases
ProteinModelPortal	A6TEQ3.
SMR	A6TEQ3. Positions 1-312.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
STRING	272620.KPN_03644.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABR79037; ABR79037; KPN_03644.
GeneID	5342243.
KEGG	kpn:KPN_03644.
PATRIC	20461713. VBIKlePne13394_3706.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0039.
HOGENOM	HOG000213792.
KO	K00024.
OMA	VEVKGFA.
OrthoDB	EOG6091FG.
ProtClustDB	PRK05086.
Enzyme and pathway databases
BioCyc	KPNE272620:GKDC-3681-MONOMER.
Family and domain databases
Gene3D	3.40.50.720. 1 hit. 3.90.110.10. 1 hit.
HAMAP	MF_01516. Malate_dehydrog_1.
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR001252. Malate_DH_AS. IPR010097. Malate_DH_type1. IPR023958. Malate_DH_type1_bac. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11540. PTHR11540. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAM	SSF56327. SSF56327. 1 hit.
TIGRFAMs	TIGR01772. MDH_euk_gproteo. 1 hit.
PROSITE	PS00068. MDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDH_KLEP7

Accession

Primary (citable) accession number: A6TEQ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 21, 2007
Last modified:	February 19, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program