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A6TD45

- CYSG1_KLEP7

UniProt

A6TD45 - CYSG1_KLEP7

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Protein
Siroheme synthase 1
Gene
cysG1, KPN78578_30550, KPN_03115
Organism
Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
Status
Reviewed - Annotation score: 5 out of 5 - Protein inferred from homologyi

Functioni

Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.UniRule annotation
S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.UniRule annotation
Precorrin-2 + NAD+ = sirohydrochlorin + NADH.UniRule annotation
Siroheme + 2 H+ = sirohydrochlorin + Fe2+.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei224 – 2241S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Active sitei247 – 2471Proton acceptor By similarity
Active sitei269 – 2691Proton donor By similarity
Binding sitei305 – 3051S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding sitei382 – 3821S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding sitei411 – 4111S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi22 – 232NAD By similarity
Nucleotide bindingi43 – 442NAD By similarity

GO - Molecular functioni

  1. NAD binding Source: InterPro
  2. precorrin-2 dehydrogenase activity Source: UniProtKB-HAMAP
  3. sirohydrochlorin ferrochelatase activity Source: UniProtKB-EC
  4. uroporphyrin-III C-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. cobalamin biosynthetic process Source: UniProtKB-HAMAP
  2. siroheme biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Methyltransferase, Oxidoreductase, Transferase

Keywords - Biological processi

Cobalamin biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

NAD, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciKPNE272620:GKDC-3115-MONOMER.
UniPathwayiUPA00148; UER00211.
UPA00148; UER00222.
UPA00262; UER00211.
UPA00262; UER00222.
UPA00262; UER00376.

Names & Taxonomyi

Protein namesi
Recommended name:
Siroheme synthase 1
Including the following 3 domains:
Uroporphyrinogen-III C-methyltransferase 1 (EC:2.1.1.107)
Short name:
Urogen III methylase 1
Alternative name(s):
SUMT 1
Uroporphyrinogen III methylase 1
Short name:
UROM 1
Precorrin-2 dehydrogenase 1 (EC:1.3.1.76)
Sirohydrochlorin ferrochelatase 1 (EC:4.99.1.4)
Gene namesi
Name:cysG1
Ordered Locus Names:KPN78578_30550
ORF Names:KPN_03115
OrganismiKlebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
Taxonomic identifieri272620 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella
ProteomesiUP000000265: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 471471Siroheme synthase 1UniRule annotation
PRO_0000330518Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei128 – 1281Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi272620.KPN_03115.

Structurei

3D structure databases

ProteinModelPortaliA6TD45.
SMRiA6TD45. Positions 1-456.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 203203precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase By similarity
Add
BLAST
Regioni215 – 471257Uroporphyrinogen-III C-methyltransferase By similarity
Add
BLAST
Regioni300 – 3023S-adenosyl-L-methionine binding By similarity
Regioni330 – 3312S-adenosyl-L-methionine binding By similarity

Sequence similaritiesi

In the C-terminal section; belongs to the precorrin methyltransferase family.

Phylogenomic databases

eggNOGiCOG0007.
HOGENOMiHOG000290518.
KOiK02302.
OrthoDBiEOG6DRPFR.

Family and domain databases

Gene3Di1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_01646. Siroheme_synth.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PfamiPF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
PROSITEiPS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6TD45-1 [UniParc]FASTAAdd to Basket

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MDYLPLFAEL KQRPVLVIGG GEIAERKIKF LLRAQAQVQV VAETLSPALA    50
DLAARQALSW RATEFSDSLV DDVFLVIAAT EDDALNQRVF AAANARYRLV 100
NVVDNQALCS FVFPSIVDRS PLLVAISSSG KAPVLSRILR EKIEALLPTN 150
LGRLAESASY WRNHLKTRLT TTEARRRFWE RVFTGRFASL MVAGNSAEAE 200
KALQDELDKP ERETGEIILV GAGPGDAGLL TLRGLQAIQQ ADVVFHDHLV 250
TQPVLELVRR DAELICVGKR AGEHSVPQHE TNQLLVEAAK AGKTVVRLKG 300
GDPFIFGRGA EELQAAAEAG IPFQVVPGVT AAAGATAYAG IPLTHRDYAQ 350
SAVFVTGHYK PDSAPFDWSL LAKSQQTLAI YMGTMKAAEI SAQLIAHGRD 400
SDTPVAVISR GTRDDQQTIT GTLQQLEHLA KDAPMPALLV VGEVVQLHQQ 450
LAWFQHTSSA EGFNASVVNL A 471
Length:471
Mass (Da):50,986
Last modified:April 29, 2008 - v2
Checksum:i9C9883AF252ED47A
GO

Sequence cautioni

The sequence ABR78516.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000647 Genomic DNA. Translation: ABR78516.1. Different initiation.
RefSeqiYP_001336746.1. NC_009648.1.

Genome annotation databases

EnsemblBacteriaiABR78516; ABR78516; KPN_03115.
GeneIDi5341891.
KEGGikpn:KPN_03115.
PATRICi20460546. VBIKlePne13394_3131.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000647 Genomic DNA. Translation: ABR78516.1 . Different initiation.
RefSeqi YP_001336746.1. NC_009648.1.

3D structure databases

ProteinModelPortali A6TD45.
SMRi A6TD45. Positions 1-456.
ModBasei Search...

Protein-protein interaction databases

STRINGi 272620.KPN_03115.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABR78516 ; ABR78516 ; KPN_03115 .
GeneIDi 5341891.
KEGGi kpn:KPN_03115.
PATRICi 20460546. VBIKlePne13394_3131.

Phylogenomic databases

eggNOGi COG0007.
HOGENOMi HOG000290518.
KOi K02302.
OrthoDBi EOG6DRPFR.

Enzyme and pathway databases

UniPathwayi UPA00148 ; UER00211 .
UPA00148 ; UER00222 .
UPA00262 ; UER00211 .
UPA00262 ; UER00222 .
UPA00262 ; UER00376 .
BioCyci KPNE272620:GKDC-3115-MONOMER.

Family and domain databases

Gene3Di 1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPi MF_01646. Siroheme_synth.
InterProi IPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view ]
Pfami PF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view ]
PIRSFi PIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMi SSF53790. SSF53790. 1 hit.
TIGRFAMsi TIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
PROSITEi PS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700721 / MGH 78578.

Entry informationi

Entry nameiCYSG1_KLEP7
AccessioniPrimary (citable) accession number: A6TD45
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: April 29, 2008
Last modified: June 11, 2014
This is version 51 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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