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Protein

tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ

Gene

trmJ

Organism
Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA.By similarity

Catalytic activityi

S-adenosyl-L-methionine + cytidine(32) in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine(32) in tRNA.By similarity
S-adenosyl-L-methionine + uridine(32) in tRNA = S-adenosyl-L-homocysteine + 2'-O-methyluridine(32) in tRNA.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei114S-adenosyl-L-methionine; via amide nitrogenBy similarity1
Binding sitei134S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJBy similarity (EC:2.1.1.200By similarity)
Alternative name(s):
tRNA (cytidine(32)/uridine(32)-2'-O)-methyltransferaseBy similarity
tRNA Cm32/Um32 methyltransferaseBy similarity
Gene namesi
Name:trmJ
Ordered Locus Names:KPN78578_28130
ORF Names:KPN_02864
OrganismiKlebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
Taxonomic identifieri272620 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeKlebsiella
Proteomesi
  • UP000000265 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003138571 – 244tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJAdd BLAST244

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi272620.KPN_02864

Structurei

3D structure databases

ProteinModelPortaliA6TCF3
SMRiA6TCF3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni79 – 81S-adenosyl-L-methionine bindingBy similarity3
Regioni141 – 143S-adenosyl-L-methionine bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CVS Bacteria
COG0565 LUCA
HOGENOMiHOG000229628
KOiK15396
OMAiPIMNLSH

Family and domain databases

Gene3Di3.40.1280.10, 1 hit
InterProiView protein in InterPro
IPR029028 Alpha/beta_knot_MTases
IPR004384 rRNA_MeTrfase_TrmH_1
IPR001537 SpoU_MeTrfase
IPR029026 tRNA_m1G_MTases_N
PANTHERiPTHR42786 PTHR42786, 1 hit
PfamiView protein in Pfam
PF00588 SpoU_methylase, 1 hit
PIRSFiPIRSF004808 LasT, 1 hit
SUPFAMiSSF75217 SSF75217, 1 hit
TIGRFAMsiTIGR00050 rRNA_methyl_1, 1 hit

Sequencei

Sequence statusi: Complete.

A6TCF3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLQNIRIVLV ETSHTGNMGS VARAMKTMGL TNLWLVNPLV KPDSQAIALA
60 70 80 90 100
AGASDVIGNA QIVDTLDEAL AGCSLVVGTS ARSRTLPWPM LDPRECGLKS
110 120 130 140 150
VAEGQHAPVA LVFGRERVGL TNDELQKCHY HVAIAANPEY SSLNLAMAVQ
160 170 180 190 200
VIAYEVRMAW LAAQEQAQPA VEHEEAPYPL VDDLERFYDH LEQTLLATGF
210 220 230 240
IRPNHPGQVM NKLRRLFTRA RPESQELNIL RGMLASIEQQ NKGK
Length:244
Mass (Da):26,798
Last modified:August 21, 2007 - v1
Checksum:i721E0FDE9B054326
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000647 Genomic DNA Translation: ABR78274.1
RefSeqiWP_002913994.1, NC_009648.1

Genome annotation databases

EnsemblBacteriaiABR78274; ABR78274; KPN_02864
KEGGikpn:KPN_02864

Similar proteinsi

Entry informationi

Entry nameiTRMJ_KLEP7
AccessioniPrimary (citable) accession number: A6TCF3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 21, 2007
Last modified: April 25, 2018
This is version 55 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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