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Protein

tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ

Gene

trmJ

Organism
Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA.By similarity

Catalytic activityi

S-adenosyl-L-methionine + cytidine(32) in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine(32) in tRNA.
S-adenosyl-L-methionine + uridine(32) in tRNA = S-adenosyl-L-homocysteine + 2'-O-methyluridine(32) in tRNA.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei114 – 1141S-adenosyl-L-methionine; via carbonyl oxygenBy similarity
Binding sitei134 – 1341S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei143 – 1431S-adenosyl-L-methionine; via amide nitrogenBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciKPNE272620:GKDC-2864-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ (EC:2.1.1.200)
Alternative name(s):
tRNA (cytidine(32)/uridine(32)-2'-O)-methyltransferase
tRNA Cm32/Um32 methyltransferase
Gene namesi
Name:trmJ
Ordered Locus Names:KPN78578_28130
ORF Names:KPN_02864
OrganismiKlebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
Taxonomic identifieri272620 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella
Proteomesi
  • UP000000265 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 244244tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJPRO_0000313857Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi272620.KPN_02864.

Structurei

3D structure databases

ProteinModelPortaliA6TCF3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CVS. Bacteria.
COG0565. LUCA.
HOGENOMiHOG000229628.
KOiK15396.
OMAiELQRCHF.

Family and domain databases

Gene3Di3.40.1280.10. 1 hit.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR004384. rRNA_MeTrfase_TrmH_1.
IPR001537. SpoU_MeTrfase.
IPR029026. tRNA_m1G_MTases_N.
[Graphical view]
PfamiPF00588. SpoU_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF004808. LasT. 1 hit.
SUPFAMiSSF75217. SSF75217. 1 hit.
TIGRFAMsiTIGR00050. rRNA_methyl_1. 1 hit.

Sequencei

Sequence statusi: Complete.

A6TCF3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLQNIRIVLV ETSHTGNMGS VARAMKTMGL TNLWLVNPLV KPDSQAIALA
60 70 80 90 100
AGASDVIGNA QIVDTLDEAL AGCSLVVGTS ARSRTLPWPM LDPRECGLKS
110 120 130 140 150
VAEGQHAPVA LVFGRERVGL TNDELQKCHY HVAIAANPEY SSLNLAMAVQ
160 170 180 190 200
VIAYEVRMAW LAAQEQAQPA VEHEEAPYPL VDDLERFYDH LEQTLLATGF
210 220 230 240
IRPNHPGQVM NKLRRLFTRA RPESQELNIL RGMLASIEQQ NKGK
Length:244
Mass (Da):26,798
Last modified:August 21, 2007 - v1
Checksum:i721E0FDE9B054326
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000647 Genomic DNA. Translation: ABR78274.1.
RefSeqiWP_002913994.1. NC_009648.1.

Genome annotation databases

EnsemblBacteriaiABR78274; ABR78274; KPN_02864.
KEGGikpn:KPN_02864.
PATRICi20460034. VBIKlePne13394_2884.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000647 Genomic DNA. Translation: ABR78274.1.
RefSeqiWP_002913994.1. NC_009648.1.

3D structure databases

ProteinModelPortaliA6TCF3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272620.KPN_02864.

Protocols and materials databases

DNASUi5342773.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABR78274; ABR78274; KPN_02864.
KEGGikpn:KPN_02864.
PATRICi20460034. VBIKlePne13394_2884.

Phylogenomic databases

eggNOGiENOG4105CVS. Bacteria.
COG0565. LUCA.
HOGENOMiHOG000229628.
KOiK15396.
OMAiELQRCHF.

Enzyme and pathway databases

BioCyciKPNE272620:GKDC-2864-MONOMER.

Family and domain databases

Gene3Di3.40.1280.10. 1 hit.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR004384. rRNA_MeTrfase_TrmH_1.
IPR001537. SpoU_MeTrfase.
IPR029026. tRNA_m1G_MTases_N.
[Graphical view]
PfamiPF00588. SpoU_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF004808. LasT. 1 hit.
SUPFAMiSSF75217. SSF75217. 1 hit.
TIGRFAMsiTIGR00050. rRNA_methyl_1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRMJ_KLEP7
AccessioniPrimary (citable) accession number: A6TCF3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 21, 2007
Last modified: September 7, 2016
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.