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A6TC19

- FADJ_KLEP7

UniProt

A6TC19 - FADJ_KLEP7

Protein

Fatty acid oxidation complex subunit alpha

Gene

fadJ

Organism
Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 56 (01 Oct 2014)
      Sequence version 1 (21 Aug 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities.UniRule annotation

    Catalytic activityi

    (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotation
    (S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotation
    (S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei118 – 1181Important for catalytic activityUniRule annotation
    Sitei140 – 1401Important for catalytic activityUniRule annotation

    GO - Molecular functioni

    1. 3-hydroxyacyl-CoA dehydrogenase activity Source: UniProtKB-HAMAP
    2. 3-hydroxybutyryl-CoA epimerase activity Source: UniProtKB-HAMAP
    3. enoyl-CoA hydratase activity Source: UniProtKB-HAMAP
    4. NAD binding Source: InterPro

    GO - Biological processi

    1. fatty acid beta-oxidation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Isomerase, Lyase, Oxidoreductase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid degradation, Lipid metabolism

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciKPNE272620:GKDC-2723-MONOMER.
    UniPathwayiUPA00659.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fatty acid oxidation complex subunit alphaUniRule annotation
    Including the following 2 domains:
    Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimeraseUniRule annotation (EC:4.2.1.17UniRule annotation, EC:5.1.2.3UniRule annotation)
    3-hydroxyacyl-CoA dehydrogenaseUniRule annotation (EC:1.1.1.35UniRule annotation)
    Gene namesi
    Name:fadJUniRule annotation
    Ordered Locus Names:KPN78578_26790
    ORF Names:KPN_02723
    OrganismiKlebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
    Taxonomic identifieri272620 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella
    ProteomesiUP000000265: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 714714Fatty acid oxidation complex subunit alphaPRO_1000069488Add
    BLAST

    Interactioni

    Subunit structurei

    Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI).UniRule annotation

    Protein-protein interaction databases

    STRINGi272620.KPN_02723.

    Structurei

    3D structure databases

    ProteinModelPortaliA6TC19.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 190190Enoyl-CoA hydrataseUniRule annotationAdd
    BLAST
    Regioni306 – 7144093-hydroxyacyl-CoA dehydrogenaseUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.UniRule annotation
    In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1250.
    HOGENOMiHOG000261346.
    KOiK01782.
    OMAiPFRYMDT.
    OrthoDBiEOG6M9F0M.

    Family and domain databases

    Gene3Di1.10.1040.10. 2 hits.
    3.40.50.720. 1 hit.
    3.90.226.10. 1 hit.
    HAMAPiMF_01617. FadJ.
    InterProiIPR006180. 3-OHacyl-CoA_DH_CS.
    IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
    IPR006108. 3HC_DH_C.
    IPR008927. 6-PGluconate_DH_C-like.
    IPR029045. ClpP/crotonase-like_dom.
    IPR001753. Crotonase_core_superfam.
    IPR013328. DH_multihelical.
    IPR012802. FadJ.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00725. 3HCDH. 2 hits.
    PF02737. 3HCDH_N. 1 hit.
    PF00378. ECH. 1 hit.
    [Graphical view]
    SUPFAMiSSF48179. SSF48179. 2 hits.
    SSF52096. SSF52096. 1 hit.
    TIGRFAMsiTIGR02440. FadJ. 1 hit.
    PROSITEiPS00067. 3HCDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A6TC19-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDTVSAFKLE VRADKIAVIT IDAPGEKMNT LKAEFGSQVR GLIRQLRDDK    50
    SVRGVVFISA KADNFIAGAD INMIARCRSA QEAEALARQG QQIMAEIHGL 100
    SIPVIAAIHG ACLGGGLELA LACHGRICSD DEKTRLGLPE VQLGLLPGSG 150
    GTQRLPRLIG VSTALDMMLT GKQLRPRQAL KAGLVDEVVP QAILLQAAVE 200
    LALKGRPTSR EVPVRERVLA GPLGRHLLFQ FVGKQTQRKT QGNYPAVKRI 250
    LQVVENGLAH GCSSGYAEEA RAFGELAMSP QSQALRSIFF ASTDLKKDPG 300
    AEAGPGSLRS VAVLGGGLMG GGIAYVTACK GGLPVRIKDI QPRGINHALK 350
    YSWDLLNKQV RQRRLRPVER DRQMALISGT TDYQGFAHRD VVIEAVFEDL 400
    ALKQRMVSEV EQYGGPQTIF ASNTSSLPIG DIAAHASRPG QVIGLHFFSP 450
    VEKMPLVEMI PHKGTDPQTI ATVVQLAKRQ GKTPIVVADK AGFYVNRILA 500
    PYINEAMRLL VEGEPIEVID NALVKFGFPV GPIQLLDEVG IDTGTKIIPV 550
    LESAFGERFS PPANIIDAIL KDDRKGRKNN RGFYLYETKG RKSKKRPDPA 600
    VYPLLGIGRP QSRLSAQQVA ERCVMMMLNE AARCFDEQII RSARDGDIGA 650
    VFGIGFPPFL GGPFRYMDTI GAGEVAAILQ RLAAQFGPRF TPCDTLLRMA 700
    EQGTTFWPAD ERLT 714
    Length:714
    Mass (Da):77,425
    Last modified:August 21, 2007 - v1
    Checksum:iF2701ED8E2714C57
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000647 Genomic DNA. Translation: ABR78140.1.
    RefSeqiWP_015958773.1. NC_009648.1.
    YP_001336370.1. NC_009648.1.

    Genome annotation databases

    EnsemblBacteriaiABR78140; ABR78140; KPN_02723.
    GeneIDi5340641.
    KEGGikpn:KPN_02723.
    PATRICi20459744. VBIKlePne13394_2746.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000647 Genomic DNA. Translation: ABR78140.1 .
    RefSeqi WP_015958773.1. NC_009648.1.
    YP_001336370.1. NC_009648.1.

    3D structure databases

    ProteinModelPortali A6TC19.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272620.KPN_02723.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABR78140 ; ABR78140 ; KPN_02723 .
    GeneIDi 5340641.
    KEGGi kpn:KPN_02723.
    PATRICi 20459744. VBIKlePne13394_2746.

    Phylogenomic databases

    eggNOGi COG1250.
    HOGENOMi HOG000261346.
    KOi K01782.
    OMAi PFRYMDT.
    OrthoDBi EOG6M9F0M.

    Enzyme and pathway databases

    UniPathwayi UPA00659 .
    BioCyci KPNE272620:GKDC-2723-MONOMER.

    Family and domain databases

    Gene3Di 1.10.1040.10. 2 hits.
    3.40.50.720. 1 hit.
    3.90.226.10. 1 hit.
    HAMAPi MF_01617. FadJ.
    InterProi IPR006180. 3-OHacyl-CoA_DH_CS.
    IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
    IPR006108. 3HC_DH_C.
    IPR008927. 6-PGluconate_DH_C-like.
    IPR029045. ClpP/crotonase-like_dom.
    IPR001753. Crotonase_core_superfam.
    IPR013328. DH_multihelical.
    IPR012802. FadJ.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF00725. 3HCDH. 2 hits.
    PF02737. 3HCDH_N. 1 hit.
    PF00378. ECH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48179. SSF48179. 2 hits.
    SSF52096. SSF52096. 1 hit.
    TIGRFAMsi TIGR02440. FadJ. 1 hit.
    PROSITEi PS00067. 3HCDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700721 / MGH 78578.

    Entry informationi

    Entry nameiFADJ_KLEP7
    AccessioniPrimary (citable) accession number: A6TC19
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: August 21, 2007
    Last modified: October 1, 2014
    This is version 56 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3