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A6TC19

- FADJ_KLEP7

UniProt

A6TC19 - FADJ_KLEP7

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Protein

Fatty acid oxidation complex subunit alpha

Gene

fadJ

Organism
Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities.UniRule annotation

Catalytic activityi

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotation
(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotation
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei118 – 1181Important for catalytic activityUniRule annotation
Sitei140 – 1401Important for catalytic activityUniRule annotation

GO - Molecular functioni

  1. 3-hydroxyacyl-CoA dehydrogenase activity Source: UniProtKB-HAMAP
  2. 3-hydroxybutyryl-CoA epimerase activity Source: UniProtKB-HAMAP
  3. enoyl-CoA hydratase activity Source: UniProtKB-HAMAP
  4. NAD binding Source: InterPro

GO - Biological processi

  1. fatty acid beta-oxidation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase, Oxidoreductase

Keywords - Biological processi

Fatty acid metabolism, Lipid degradation, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciKPNE272620:GKDC-2723-MONOMER.
UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid oxidation complex subunit alphaUniRule annotation
Including the following 2 domains:
Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimeraseUniRule annotation (EC:4.2.1.17UniRule annotation, EC:5.1.2.3UniRule annotation)
3-hydroxyacyl-CoA dehydrogenaseUniRule annotation (EC:1.1.1.35UniRule annotation)
Gene namesi
Name:fadJUniRule annotation
Ordered Locus Names:KPN78578_26790
ORF Names:KPN_02723
OrganismiKlebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
Taxonomic identifieri272620 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella
ProteomesiUP000000265: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 714714Fatty acid oxidation complex subunit alphaPRO_1000069488Add
BLAST

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI).UniRule annotation

Protein-protein interaction databases

STRINGi272620.KPN_02723.

Structurei

3D structure databases

ProteinModelPortaliA6TC19.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 190190Enoyl-CoA hydrataseUniRule annotationAdd
BLAST
Regioni306 – 7144093-hydroxyacyl-CoA dehydrogenaseUniRule annotationAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.UniRule annotation
In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1250.
HOGENOMiHOG000261346.
KOiK01782.
OMAiPFRYMDT.
OrthoDBiEOG6M9F0M.

Family and domain databases

Gene3Di1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
HAMAPiMF_01617. FadJ.
InterProiIPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR012802. FadJ.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00725. 3HCDH. 2 hits.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
TIGRFAMsiTIGR02440. FadJ. 1 hit.
PROSITEiPS00067. 3HCDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6TC19-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDTVSAFKLE VRADKIAVIT IDAPGEKMNT LKAEFGSQVR GLIRQLRDDK
60 70 80 90 100
SVRGVVFISA KADNFIAGAD INMIARCRSA QEAEALARQG QQIMAEIHGL
110 120 130 140 150
SIPVIAAIHG ACLGGGLELA LACHGRICSD DEKTRLGLPE VQLGLLPGSG
160 170 180 190 200
GTQRLPRLIG VSTALDMMLT GKQLRPRQAL KAGLVDEVVP QAILLQAAVE
210 220 230 240 250
LALKGRPTSR EVPVRERVLA GPLGRHLLFQ FVGKQTQRKT QGNYPAVKRI
260 270 280 290 300
LQVVENGLAH GCSSGYAEEA RAFGELAMSP QSQALRSIFF ASTDLKKDPG
310 320 330 340 350
AEAGPGSLRS VAVLGGGLMG GGIAYVTACK GGLPVRIKDI QPRGINHALK
360 370 380 390 400
YSWDLLNKQV RQRRLRPVER DRQMALISGT TDYQGFAHRD VVIEAVFEDL
410 420 430 440 450
ALKQRMVSEV EQYGGPQTIF ASNTSSLPIG DIAAHASRPG QVIGLHFFSP
460 470 480 490 500
VEKMPLVEMI PHKGTDPQTI ATVVQLAKRQ GKTPIVVADK AGFYVNRILA
510 520 530 540 550
PYINEAMRLL VEGEPIEVID NALVKFGFPV GPIQLLDEVG IDTGTKIIPV
560 570 580 590 600
LESAFGERFS PPANIIDAIL KDDRKGRKNN RGFYLYETKG RKSKKRPDPA
610 620 630 640 650
VYPLLGIGRP QSRLSAQQVA ERCVMMMLNE AARCFDEQII RSARDGDIGA
660 670 680 690 700
VFGIGFPPFL GGPFRYMDTI GAGEVAAILQ RLAAQFGPRF TPCDTLLRMA
710
EQGTTFWPAD ERLT
Length:714
Mass (Da):77,425
Last modified:August 21, 2007 - v1
Checksum:iF2701ED8E2714C57
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000647 Genomic DNA. Translation: ABR78140.1.
RefSeqiWP_015958773.1. NC_009648.1.
YP_001336370.1. NC_009648.1.

Genome annotation databases

EnsemblBacteriaiABR78140; ABR78140; KPN_02723.
GeneIDi5340641.
KEGGikpn:KPN_02723.
PATRICi20459744. VBIKlePne13394_2746.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000647 Genomic DNA. Translation: ABR78140.1 .
RefSeqi WP_015958773.1. NC_009648.1.
YP_001336370.1. NC_009648.1.

3D structure databases

ProteinModelPortali A6TC19.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272620.KPN_02723.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABR78140 ; ABR78140 ; KPN_02723 .
GeneIDi 5340641.
KEGGi kpn:KPN_02723.
PATRICi 20459744. VBIKlePne13394_2746.

Phylogenomic databases

eggNOGi COG1250.
HOGENOMi HOG000261346.
KOi K01782.
OMAi PFRYMDT.
OrthoDBi EOG6M9F0M.

Enzyme and pathway databases

UniPathwayi UPA00659 .
BioCyci KPNE272620:GKDC-2723-MONOMER.

Family and domain databases

Gene3Di 1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
HAMAPi MF_01617. FadJ.
InterProi IPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR012802. FadJ.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF00725. 3HCDH. 2 hits.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view ]
SUPFAMi SSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
TIGRFAMsi TIGR02440. FadJ. 1 hit.
PROSITEi PS00067. 3HCDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700721 / MGH 78578.

Entry informationi

Entry nameiFADJ_KLEP7
AccessioniPrimary (citable) accession number: A6TC19
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: August 21, 2007
Last modified: November 26, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3